Embryo and cow factors affecting pregnancy per embryo transfer for multiple-service, lactating Holstein recipients
Eliab Estrada‐CortésW. OrtizR.C. ChebelElizabeth A. JannamanJames I. MossF. C. CastroA. M. ZoliniC.R. StaplesPeter J. Hansen
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Abstract:
The objective was to determine whether pregnancy success after embryo transfer (ET) during heat stress in multi-service Holstein cows depends upon characteristics of the embryo or recipient. Female embryos produced in vitro were cultured with either 0.0 (control) or 1.8 mM choline chloride and transferred fresh. Fresh embryos of undetermined breed and frozen Holstein embryos were used when experimental embryos were insufficient. Embryos were transferred 8 d after the last GnRH injection of an ovulation synchronization program. Embryo type [frozen vs. fresh, choline vs. control, unknown breed vs. (control + choline)] and characteristics of recipients (average of 190 d in milk at transfer) were evaluated. Pregnancy per ET was lower for cows receiving frozen embryos (7.0%; 3/43) than for cows receiving fresh embryos (26.7%; 32/120) but there were no differences between various types of fresh embryo. Pregnancy per ET was lower for cows diagnosed with metritis in the early postpartum period (7.1%; 2/28) than for cows without metritis (24.4%; 33/135). In conclusion, the use of frozen/thawed embryos produced in vitro and recipients which had metritis in the early postpartum period reduced the success of ET in multiple-service Holstein cows.Keywords:
Metritis
The objective of the current study was to determine the accuracy of disease detection based on daily rumination time (DRT) and activity of periparturient dairy cows. All animals were fitted with rumination/activity monitors from -21 to 21 days relative to calving. Cows that were within the lowest 25th percentile of milk yield in the first 90 d postpartum had reduced DRT, but there was no association between milk yield and activity during the periparturient period. Based on criterion created using DRT, stillbirth could be diagnosed with sensitivity and specificity of 50 and 79.7%, respectively. Two criteria could be used for diagnosis of sub-clinical hypocalcemia on the day of calving; one resulted in 66.7 and 61.3% sensitivity and specificity, and the other sensitivity and specificity of 82.7 and 49.6%, respectively. Metritis could be diagnosed 72 h after calving with a sensitivity and specificity of 75 and 93.1%, respectively. Among cows that were diagnosed with retained placenta within 24 h after calving, the DRT criterion resulted in sensitivity and specificity of 70.8 and 75%, respectively. In conclusion, automated monitoring of DRT could possibly be used as a tool for diagnosis of periparturient diseases; however, the use of DRT data to select individuals for treatment without additional diagnostic exams is likely to results in erroneous treatment of periparturient cows.
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Aim of the study: To evaluate the association between temperature-humidity index (THI) and body condition score (BCS) at calving and retained placenta (RP), puerperal metritis, clinical ketosis, and mastitis in Holstein cows in a hot environment Area of study: Northeastern Mexico. Material and methods: This is a retrospective cohort study (n= 12,102 lactations from January 2017 to December 2021) using univariate logistic regressions. The outcome variables were periparturient diseases, and the predictor variables were BCS and thermal stress at calving. Main results: Cows calving with a THI > 82 were 30% more likely (prevalence 16.8% vs 13.7%; p < 0.01) to have RP than cows whose parturition occurred with moderate or low thermal stress (THI < 82 units). Cows calving with THI > 82 had significantly increased chances of having metritis than cows calving with THI < 82 (prevalence 15.6 vs 13.4; p < 0.01). Cows calving with a THI > 82 were 1.8 times more likely to have clinical ketosis (7.6% vs 4.4%; p < 0.01) than cows calving with THI < 82 units. Cows with BCS at calving ≥ 3.5 had half the risk of having RP (prevalence 10.4 vs 19.1%, p < 0.01) than cows with BCS < 3.5. Likewise, the risk of metritis decreased (p < 0.01) with BCS ≥3.5 at calving (prevalence 10.9 vs 17.4%). Research highlights: Heat stress at calving was associated with an increased risk for RP, puerperal metritis, and clinical ketosis compared to cows undergoing mild or no heat stress at parturition. Also, cows with BCS ≥ 3.5 were less likely to present RP and metritis, but high body fatness was associated with an increased risk for clinical ketosis.
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Metritis affects up to 40% of dairy cows and it is usually treated with antibiotics. In spite of their advantages, there is an increased concern about antibiotic resistance leading to the research of alternative methods. The aim of this study was to evaluate the effects of a combination of lactic acid bacteria (LAB) on the prevalence of metritis and modulation of endometrial and neutrophil inflammatory markers in dairy cows. One hundred and thirty-five cows were enrolled 3 week before calving and randomly assigned to three treatments. Treatment groups were: (1) two intravaginal doses of LAB/wk during 3 week pre-calving (vaginal, n = 45); (2) an intra-uterine dose, once 1 d after calving (uterine, n = 44); and (3) no intervention (CTRL, n = 45). Metritis was defined as body temperature > 39.5°C and purulent vaginal discharge (> 50% pus), and diagnosed 6 d after calving. Blood samples were taken at d -14, -10, -7, -4, +1, +3, +6, and +14 relative to calving for non-esterified fatty acids (NEFA) analysis. At d -10, +1, +3, and +6 neutrophils were isolated from blood for gene expression analysis by RT-qPCR. Endometrium biopsies were taken from 30 cows, 15 from CTRL and 15 from the uterine group at d +1, +3, and +6 after calving for pro-inflammatory markers analysis by NanoString®. Vaginal treatment reduced metritis prevalence (6/45) up to 58% compared with CTRL group (14/45), but there was no difference between the uterine and CTRL group. Uterine and vaginal treatments reduced blood neutrophil gene expression. Expression of pro-inflammatory markers in the endometrium did not differ between uterine and CTRL cows. Metritic cows expressed more C-X-C motif chemokine ligand 8 (CXCL8) and interleukin 1 beta (IL1B) at d 3 than healthy cows, whereas healthy cows expressed more CXCL8 at d 1 relative to calving in the endometrium. This study shows a promising potential of LAB probiotics as a preventive treatment against metritis in dairy cows.
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This study was conducted to evaluate effects of calcium propionate on hypocalcemia, dry matter intake, body condition score, milk production and reproductive disorders in dairy cows. Twenty four multiparous Holstein cows were sorted by parity, body condition score (BCS) in close-up period and season of calving and assigned to one of the three treatments. The cows in treatment 1 (T1) received two drenches at calving and 24h after calving. The cows in treatment 2 (T2) received three drenches at calving, 24h after calving and 7 days after calving. The cows in treatment 3 (T3) were the control. Each drench contained 143g of calcium as calcium propionate (0.68kg). Parameters studied were serum calcium, glucose and nonesterified fatty acid (NEFA) concentrations, dry matter intake (DMI), BCS, milk production (MP), incidence of retained placenta (RP) and metritis. Milk fever developed in 5 of 8 cows, in 3 of 8 cows and in 3 of 8 cows in T1, T2 and T3, respectively, at calving. There was no cow with milk fever in T1 and T2 at 4h after second drench (about 28h after calving) but 3 of 8 cows in T3 had still milk fever at this time. The cows receiving two drenches recovered from milk fever in a shorter term as compared to the cows in T3. There were no differences among treatments for DMI, BCS, MP, RP, serum glucose and NEFA concentrations during the experimental period. There was no difference for metritis between T1 and T3 but incidence of metritis in T2 was significantly lower as compared to T3 (P<0.05). Two drenches of calcium propionate were beneficial in treating milk fever and three drenches of calcium propionate were considered to have had a preventive effect for metritis.
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Blood samples were collected from 41 advanced pregnant cows on 15 days prepartum (–15d), calving day (0d), 15 days (15d) and 30 days (30d) postpartum to study the nitric oxide profile in cows with Retained placenta (ROP), Clinical metritis (CM), Clinical endometritis (CE), Cervicitis (CT) and Delayed involution of uterus (DIU) during the periparturient period. The blood serum was used for estimation of nitric oxide (NO) using microassay. The nitric oxide level (μM) in the serum of cows was nonsignificantly higher for ROP, CM and CE than normal at –15d and the corresponding values were 31.88±5.01, 30.30±4.41, 32.08±7.56 and 22.31±2.69 at calving and 13.89±2.72, 14.70±4.84, 7.08±2.13 and 11.94±1.46 at 30d pp. The NO level increased from –15d to the day of calving (0d), thereafter a significant decrease was observed from 0d to postpartum days (15d and 30d) in ROP, CM, CE and normal cows. The significantly higher NO level in cows around the day of calving in all cows indicates its role on the physiology of calving. It is evident from the study that NO level declined significantly from calving to 15d and 30d postpartum in all postpartum cows irrespective of inflammatory status of the uterus. However, the level of NO in periparturient cows is not associated with the development of postpartum uterine diseases.
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Involution (esoterism)
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The objective of the present study was to assess the effectiveness of an intravaginal thermometer in the field prediction of the second stage of labor and to determine its impact on the health of dams and newborn calves. Holstein cows (n = 241) were randomly selected about 5 (mean ± SD: 4.7 ± 2.0) days before the expected date of calving and the thermometer was inserted into the vagina. Another 113 cattle served as controls. There was no false alarm during the experiment. The risk of dystocia (Score >1) was 1.9 times higher, the prevalence of stillbirth was 19.8 times higher, the risk of retained fetal membranes (RFM) was 2.8 times higher and the risk of clinical metritis was 10.5 times higher in the control group than in the experimental group. The prevalence of stillbirth was 7 times higher in cows with dystocia compared to cows with eutocia. The presence of dystocia and stillbirth increased the risk of RFM 4 and 5 times, respectively. The occurrence of RFM increased the risk of development of clinical metritis with a 22 times higher odds. The results indicate that the use of calving alert systems not only facilitates controlling the time of parturition and providing prompt and appropriate calving assistance but also decreases the number of dystocia cases and improves reproductive efficiency, postpartum health of the dam and newborn calf survival.
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This study aimed to evaluate the effects of different pre-calving body conditions on some metabolic indices, health status and reproductive disorders in dairy cows. The study was conducted on a dairy farm in Eastern Slovakia during the pre-partum period (–1 to –10 DIM) and post-partum period (1 to 14 DIM). Total of 84 cows allotted in 3 groups were submitted to clinical examination and body condition score (BCS) evaluation. At the same intervals blood samples were collected for determination of beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), and calcium levels. During the postpartum period the incidence of reproductive diseases, such as metritis, retained placenta, metabolic disorders, such as milk fever, left displacement of abomasum and orthopaedic disorders were recorded. The strongest lipid mobilisation (NEFA elevation), ketone body production, and blood calcium drop were determined in postpartum dairy cows with the highest prepartum body condition score. In conclusion, the dairy cows with different BCS hade a different metabolic response during the postpartum period. The overcondition was connected with stronger lipid mobilisation and calcium drop after calving. Moreover, the higher BCS could contribute to higher incidence of production diseases.
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Retained placenta
NEFA
Ketosis
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Total mixed ration
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