Multimodality endoscopic imaging technology for visualization of layered architecture and vasculature
1
Citation
20
Reference
10
Related Paper
Citation Trend
Abstract:
Endoscopic imaging technologies, such as endoscopic optical coherence tomography (OCT), near infrared (NIR) fluorescence, photoacoustic (PA), and ultrasound (US) have been used to investigate vascular and morphological changes as hallmarks of early cancer in the gastrointestinal (GI) tract. Here, we developed two multimodality imaging systems which are integrated PA/US and integrated OCT/NIR fluorescence which can obtain layered architecture and vasculature simultaneously. In vivo imaging of rectum wall from Sprague Dawley (SD) rats with these two imaging systems were demonstrated. Both imaging systems enable the use of one imaging probe for performing two different imaging, thereby improving prognosis by early detection and reducing costs. For integrated PA/US, the architectural morphology and vasculature of the rectum wall were visualized without the usage of contrast agent, but slow imaging speed and usage of match medium are the main limitations for clinical translation. With regard to the integrated OCT/NIR fluorescence, it is able to perform high speed imaging, however the addition of contrast agent and limited imaging depth are the main concern for clinical application.Keywords:
Fluorescence-lifetime imaging microscopy
Molecular Imaging
Molecular imaging is a newly rising branches of science which comes from molecular biology technology and modern medical imaging,and it would be the development tendency of mendiacl imaging. This article briefly introduces the basal principle of molecular imaging,the difference and correlation between molecular imaging and traditional medical imaging and the development prospect of medical imaging.
Molecular Imaging
Imaging technology
Imaging science
Cite
Citations (0)
Other SectionsABSTRACTINTRODUCTIONIN VIVO MOLECULAR IMAGINGIN VIVO SINGLE CELL IMAGINGACKNOWLEDGEMENTSCONFLICTS OF INTERESTFIGURESTABLEREFERENCES
Molecular Imaging
Cite
Citations (6)
Photoacoustic (PA) imaging as a new hybrid imaging modality holds great promise for real-time in vivo monitoring of biological processes with deep tissue penetration and high spatial resolution. To endow PA imaging with the ability to provide real-time molecular information at disease sites, molecular probes that can change their PA signals responding to the target or event of interest have to be developed. This review focuses on the recent development of smart activatable PA probes for molecular imaging. A brief summary of PA imaging agents is given first, followed by the detailed discussion of the contemporary design approaches toward activatable PA probes for different imaging applications. At last, the current challenges are highlighted.
Molecular Imaging
Molecular probe
Modality (human–computer interaction)
Cite
Citations (163)
Optical and photoacoustic imaging plays an important role in biomedical applications owing to its noninvasiveness and high resolution. Fluorescence imaging and photoacoustic imaging emerge as powerful tools to deconstruct molecular information and investigate biological processes in vivo. Despite great progress has been achieved in chemical probe synthesis, how to design probes with optimal fluorescence or photoacoustic imaging performance to dynamically visualize the biological process in vivo still faces challenges. From this perspective, we will focus on the advanced development of fluorescence and photoacoustic imaging in vivo. Furthermore, concerns and prospects for future imaging in vivo will be demonstrated.
Fluorescence-lifetime imaging microscopy
Photoacoustic tomography
Molecular Imaging
Cite
Citations (30)
In vivo imaging of molecular events in small animals has great potential to impact basic science and drug development. For this reason, several imaging technologies have been adapted to small animal research, including X-ray, magnetic resonance, and radioisotope imaging. Despite this plethora of visualization techniques, fluorescence imaging is emerging as an important alternative because of its operational simplicity, safety, and cost-effectiveness. Fluorescence imaging has recently become particularly interesting because of advances in fluorescent probe technology, including targeted fluorochromes as well as fluorescent “switches” sensitive to specific biochemical events. While past biological investigations using fluorescence have focused on microscopic examination of ex vivo, in vitro, or intravital specimens, techniques for macroscopic fluorescence imaging are now emerging for in vivo molecular imaging applications. This review illuminates fluorescence imaging technologies that hold promise for small animal imaging. In particular we focus on planar illumination techniques, also known as Fluorescence Reflectance Imaging (FRI), and discuss its performance and current use. We then discuss fluorescence molecular tomography (FMT), an evolving technique for quantitative three-dimensional imaging of fluorescence in vivo. This technique offers the promise of non-invasively quantifying and visualizing specific molecular activity in living subjects in three dimensions.
Molecular Imaging
Fluorescence-lifetime imaging microscopy
Cite
Citations (209)
In vivo molecular imaging is a powerful tool to analyze the human body. Precision medicine is receiving high attention these days, and molecular imaging plays an important role as companion diagnostics in precision medicine. Nuclear imaging with PET or SPECT and optical imaging technologies are used for in vivo molecular imaging. Nuclear imaging is superior for quantitative imaging, and whole-body analysis is possible even for humans. Optical imaging is superior due to its ease of use, and highly targeted specific imaging is possible with activatable agents. However, with optical imaging using fluorescence, it is difficult to obtain a signal from deep tissue and quantitation is difficult due to the attenuation and scattering of the fluorescent signal. Recently, to overcome these issues, optoacoustic imaging has been used in in vivo imaging. In this article, we review in vivo molecular imaging with nuclear and optical imaging and discuss their utility for precision medicine.
Molecular Imaging
Nuclear medicine imaging
Fluorescence-lifetime imaging microscopy
Nuclear imaging
Imaging science
Molecular probe
Cite
Citations (16)
Molecular imaging is a powerful tool to visualize and characterize biological processes at the cellular and molecular level in vivo . In most molecular imaging approaches, probes are used to bind to disease‐specific biomarkers highlighting disease target sites. In recent years, a new subset of molecular imaging probes, known as bioresponsive molecular probes, has been developed. These probes generally benefit from signal enhancement at the site of interaction with its target. There are mainly two classes of bioresponsive imaging probes. The first class consists of probes that show direct activation of the imaging label (from “off” to “on” state) and have been applied in optical imaging and magnetic resonance imaging (MRI). The other class consists of probes that show specific retention of the imaging label at the site of target interaction and these probes have found application in all different imaging modalities, including photoacoustic imaging and nuclear imaging. In this review, we present a comprehensive overview of bioresponsive imaging probes in order to discuss the various molecular imaging strategies. The focus of the present article is the rationale behind the design of bioresponsive molecular imaging probes and their potential in vivo application for the detection of endogenous molecular targets in pathologies such as cancer and cardiovascular disease. Copyright © 2015 John Wiley & Sons, Ltd.
Molecular Imaging
Molecular probe
Cite
Citations (29)
Molecular imaging visualizes, characterizes, and measures biological processes at the molecular and cellular level. In oncology, molecular imaging is an important technology to guide integrated and precise diagnosis and treatment. Photoacoustic imaging is mainly divided into three categories: photoacoustic microscopy, photoacoustic tomography and photoacoustic endoscopy. Different from traditional imaging technology, which uses the physical properties of tissues to detect and identify diseases, photoacoustic imaging uses the photoacoustic effect to obtain the internal information of tissues. During imaging, lasers excite either endogenous or exogenous photoacoustic contrast agents, which then send out ultrasonic waves. Currently, photoacoustic imaging in conjunction with targeted photoacoustic contrast agents is frequently employed in the research of tumor molecular imaging. In this study, we will examine the latest advancements in photoacoustic imaging technology and targeted photoacoustic contrast agents, as well as the developments in tumor molecular imaging research.
Molecular Imaging
Photoacoustic tomography
Imaging technology
Photoacoustic Doppler effect
Photoacoustic Spectroscopy
Cite
Citations (15)
Advancements in medical imaging have brought about unprecedented changes in the in vivo assessment of cancer. Positron emission tomography, single photon emission computed tomography, optical imaging, and magnetic resonance imaging are the primary tools being developed for oncologic imaging. These techniques may still be in their infancy, as recently developed chemical molecular probes for each modality have improved in vivo characterization of physiologic and molecular characteristics. Herein, we discuss advances in these imaging techniques, and focus on the major design strategies with which molecular probes are being developed.
Molecular Imaging
Modality (human–computer interaction)
Emission computed tomography
Characterization
Molecular probe
Cite
Citations (69)
Molecular imaging is a newly emerging and rapidly developing biomedical imaging field in which the modern technologies and instruments are being merged to study biological and medical processes, as well as diagnosing and managing diseases. In the study of molecular imaging, the three research focuses are the imaging techniques, specific molecular probes, and molecular imaging applications in pathology and pharmacology. Therefore, novel molecular imaging theories and algorithms, new molecular probes, multimodality molecular imaging prototype systems, experiments, and biomedical applications are introduced as a whole project.
Molecular Imaging
Multimodality
Cite
Citations (93)