Identification of a major QTL on chromosome arm 2AL for reducing yellow rust severity from a Chinese wheat landrace with evidence for durable resistance
20
Citation
96
Reference
10
Related Paper
Citation Trend
Keywords:
Rust (programming language)
Marker-Assisted Selection
Validated Molecular Marker for Downy Mildew Disease Resistance Breeding of Sunflower: A Short Review
The oomycete pathogen Plasmopara halstedii responsible for sunflower downy mildew (DM), that is a significant and important disease that greatly affects the economy. As of now, there is no non-race-specific resistance for this disease and breeders are depended on race-specific resistance to control DM disease. On the other hand, using conventional breeding procedure introgression of the DM resistance genes is a long-term task due to the highly virulent and aggressive nature of the P. halstedii pathogen. Molecular markers that can be applied at the seedling stage, offers rapid response for selection with higher precision as well as a lower cost. There are currently 36 downy mildew resistance genes (R genes), designated as Pl (Pl1-Pl36, Plhra, and PlArg, in sunflowers, each with a unique linkage group (LGs). The availability of DM resistance genomic data of sunflower, related to Single Nucleotide Polymorphisms (SNP) based markers with mine allelic diversity maximize the opportunity of utilizing Marker assisted selection (MAS) techniques for downy mildew resistance breeding. This review highlights the available genetic marker and their utilization at MAS techniques for enhancing downy mildew disease resistant breeding program of sunflowers.
Marker-Assisted Selection
Introgression
Oomycete
Molecular marker
Cite
Citations (1)
Molecular marker of disease resistance gene is stable accurate and efficient. It's application in marker-assisted selection can accelerate the conventional breeding process and raise its efficiency through the direct selection on genotype but not phenotype. Outline of development of marker-assisted selection in plant breeding for disease resistance was reviewed in this paper. Technology of various DNA molecular tagging and selection method of molecular marker linked to crop disease resistance gene was introduced. Current status in the area of molecular tagging on disease resistance genes in sugarcane is also enclosed. At the same time prospects of molecular marker-assisted selection in sugarcane breeding for disease resistance were forecasted.
Marker-Assisted Selection
Molecular marker
Molecular breeding
Plant Breeding
Disease resistant
Cite
Citations (1)
The mega wheat variety HD2967 was improved for leaf and stripe rust resistance by marker-assisted backcross breeding. After its release in 2011, HD2967 became susceptible to stripe rust and moderately susceptible to leaf rust. The leaf rust resistance gene LrTrk was transferred into HD2967 from the durum wheat genotype Trinakria. Then, HD2967 was crossed with Trinakria to produce F1 plant foreground selection for LrTrk and background selection for the recurrent parent genotype was carried out in BC1F1, BC2F1 and BC2F2 generations. Foreground selection was carried out with the linked marker Xgwm234, while polymorphic SSR markers between parents were used for background selection. Background selection resulted in the rapid recovery of the recurrent parent genome. A morphological evaluation of 6 near isogenic lines (NILs)-2 resistant to leaf and stripe rust, and 4 resistant to leaf rust only-showed no significant differences in yields among NILs and the recurrent parent HD2967. All of the 6 NILs showed the presence of 2NS/2AS translocation, carrying the linked genes Lr37/Sr38/Yr17 present in HD2967 and the targeted leaf rust resistance gene LrTrk. Two NILs also showed additional resistance to stripe rust. Therefore, these NILs with rust resistance and an at par yielding ability of H2967 can replace the susceptible cultivar HD2967 to reduce yield losses due to disease.
Rust (programming language)
Marker-Assisted Selection
Stripe rust
Cite
Citations (9)
One thirty one varieties of Groundnut were screened for search of multiple disease resistance against rust caused by Puccinia arachidis speg. and leaf spots caused by Cereospora arachidis Hori and Phaeoisariopsis personata . Out of these, 7 were resistant, and 11 were moderatly resistant. Percent disease severity and its' effect on yield was assessed.
Rust (programming language)
Disease resistant
Cite
Citations (1)
Genomic Selection
Marker-Assisted Selection
Plant Breeding
Molecular breeding
Disease resistant
Cite
Citations (8)
Rusts are among the most devastating fungal diseases of wheat worldwide causing major yield losses. The most effective way to control yield losses caused by rusts is by incorporating genetic resistance in wheat cultivars. In view of this, marker assisted backcrossing (MABC) was performed to transfer three highly effective genes, two for leaf rust resistance (Lr19/Sr25 and Lr24/Sr24) and one for stripe rust resistance (Yr15) into a wheat cultivar UP 2338. The Avocet/Yr15 (Yr15) was used as donor for stripe rust and FLW 8 and FLW 21 were the donors for Lr19/Sr25 and Lr24/Sr24, respectively. Two linked SSR markers, Xgwm273 for Yr15 and Xwmc221 for Lr19/Sr25 and a SCAR marker, SCS1302 for Lr24/Sr24 were used for foreground selection to select plants carrying respective gene(s). Foreground selection coupled with background selection identified plants homozygous for stripe rust and leaf rust resistance and materials have been advanced up to BC3F3 stage. Findings of this investigation show the usefulness of marker assisted selection in precise introgression of the genes for stripe rust and leaf rust resistance.
Rust (programming language)
Introgression
Marker-Assisted Selection
Background selection
Molecular marker
Cite
Citations (8)
Basmati rice grown in the Indian subcontinent is highly valued for its unique culinary qualities. Production is, however, often constrained by diseases such as bacterial blight (BB), blast and sheath blight (ShB). The present study developed Basmati rice with inbuilt resistance to BB, blast and ShB using molecular marker-assisted selection. The rice cultivar ‘Improved Pusa Basmati 1’ (carrying the BB resistance genes xa13 and Xa21) was used as the recurrent parent and cultivar ‘Tetep’ (carrying the blast resistance gene Pi54 and ShB resistance quantitative trait loci (QTL), qSBR11-1) was the donor. Marker-assisted foreground selection was employed to identify plants possessing resistance alleles in the segregating generations along with stringent phenotypic selection for faster recovery of the recurrent parent genome (RPG) and phenome (RPP). Background analysis with molecular markers was used to estimate the recovery of RPG in improved lines. Foreground selection coupled with stringent phenotypic selection identified plants homozygous for xa13, Xa21 and Pi54, which were advanced to BC2F5 through pedigree selection. Marker-assisted selection for qSBR11-1 in BC2F5 using flanking markers identified seven homozygous families. Background analysis revealed that RPG recovery was up to 89.5%. Screening with highly virulent isolates of BB, blast and ShB showed that the improved lines were resistant to all three diseases and were on a par with ‘Improved Pusa Basmati 1’ for yield, duration and Basmati grain quality. This is the first report of marker-assisted transfer of genes conferring resistance to three different diseases in rice wherein genes xa13 and Xa21 for BB resistance, Pi54 for blast resistance, and a major QTL qSBR11-1 have been combined through marker-assisted backcross breeding. In addition to offering the potential for release as cultivars, the pyramided lines will serve as useful donors of gene(s) for BB, blast and ShB in future Basmati rice breeding programmes.
Marker-Assisted Selection
Background selection
Molecular marker
Breeding program
Cite
Citations (105)
Marker-Assisted Selection
Background selection
Molecular marker
Grain Quality
Cite
Citations (240)
Background selection
Marker-Assisted Selection
Aromatic rice
Molecular marker
Pusa
Cite
Citations (150)