Clinical Significance of the Survivin Expression in Intrahepatic Cholangiocarcinoma with Hepatolithiasis
Hyung Jin JunHyung Chul KımChul Wan LimEung Jin SinGyu Seok ChoChong Woo ChuHyo Won LeeOk Pyung SongHee Kyung KimEun Suk Koh
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Keywords:
Hepatolithiasis
Intrahepatic Cholangiocarcinoma
Survivin
Clinical Significance
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Objective To investigate the expression and clinical significance of p15 in cholangiocarcinoma.Methods Supersensitive SP immunohistochemical was used to detect the expression of p15 protein in 38 cases of cholangiocarcinoma and 8 cases of biliary cyst.Results The positive rates of p15 were 52.63% in cholangiocarcinoma and 100% in biliary cyst.p15 positive expression rate in cholangiocarcinoma were significantly lower than that in biliary cyst(P0.05).The expression level of p15 protein significantly correlate with tumor grade, clinical stage and lymph node metastasis(P0.05).Conclussion p15 have close relationship with tumorgenesis,progression and malignant behavior in cholangiocarcinoma.
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Background: By studying the expression of epithelial-mesenchymal transition regulators in cholangiocarcinoma and intrahepatic duct stones, the correlation between the expression of epithelial-mesenchymal transition regulators and cholangiocarcinoma was revealed. Objective: The objective is to investigate the correlation between the expression of epithelial-mesenchymal transition (EC) regulatory factors and cholangiocarcinoma in patients with intrahepatic duct stones and cholangiocarcinoma, to investigate the relationship between clinicopathological features and prognosis, and to observe the expression of molecular markers of epithelial-mesenchymal transition (EMT) in intrahepatic duct stones and bile duct carcinoma. Methods: Twenty cases of primary cholangiocarcinoma, 20 cases of intrahepatic cholangiolithiasis complicated with cholangiocarcinoma, and 20 cases of intrahepatic cholangiolithiasis specimens were collected from the Fourth People’s Hospital and the friendly medical unit of Haikou. Immunohistochemistry was used to detect the expression differences of EMT-related molecular markers Twisit1, Twisit2, E-cadherin, N-cadherin, and Vimentin in paraffin sections of normal intrahepatic bile duct tissues and patients with intrahepatic duct stones and cholangiocarcinoma. Results: Immunohistochemical staining revealed epithelial-mesenchymal transition (EMT) in intrahepatic cholangiocarcinoma tissue, intrahepatic cholangiolithiasis with cholangiocarcinoma, intrahepatic cholangiolithiasis with normal intrahepatic cholangiolithiasis, such as Sit1, Twisit2, E-cadherin, N-cadherin, and Vimentin proteins were different. The expression of E-cadherin was decreased in cholangiocarcinoma tissue and intrahepatic cholangiolithiocarcinoma combined with cholangiocarcinoma (P 0.05). There was no difference in the expression of intrahepatic bile duct stones and EMT (P > 0.05). Conclusion: The expression of E-cadherin, the molecular marker of EMT, was down-regulated, while the expression of N-cadherin and Vimentin was up-regulated. Age, gender, depth of tumor invasion, degree of tumor differentiation and lymph node metastasis were correlated with the expression of EMT in intrahepatic cholangiocarcinoma.
Intrahepatic Cholangiocarcinoma
Intrahepatic bile ducts
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Intrahepatic Cholangiocarcinoma
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Objective To investgate the expression and the clinical significance of survivin in the primary hepatocellular carcinoma (HCC). Methods S-P immunohistochemical staining was performed to detect the expression of survivin in 50 samples of HCC tissue and 20 of normal liver tissue. Results The positive rate of survivin expression in HCC was 66. 0% (33/50) , which was significantly higher than that in normol liver tissues (negative expression) (P<0. 001). The expression of survivin was correlated with intrahepatic dissemination and multiple masses (P = 0. 019, P=0. 030). The high survivin expression was associated with the survival time less than three years(P = 0. 018). Conclusion The survivin may play certain roles in the oncogenesis and progression of HCC. Detection of the survivin can supply certain evidence on prognosis of primary hepatocellular carcinoma.
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Carcinoma hepatocellular; Survivin; Immunohistochemistry
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Objective:Investigate the expression of Survivin and its correlation with the expression of PTEN and nm23 in hilar cholangiocarcinoma.Methods:In 44 specimens of hilar cholangiocarcinoma,15 specimens of tissues near the carcinoma(the distance to the edge of tumor ≥1 centimeter and no infiltration pathologically),the expressions of Survivin,PTEN and nm23 were detected by immunohistochemistry methods.Laboratory data were then analyzed statistically together with the pathological data.Results:In 44 cases of hilar cholangiocarcinoma,the positive rate of Survivin was 63.6%,in tissues near the carcinoma the positive rate was 6.67%;The expression of Survivin in hilar cholangiocarcinoma had no association with pathological stage and lymph node metastasis(P0.05);The expression of Survivin in hilar cholangiocarcinoma was closely statistically related with the expressions of PTEN and nm23.Conclusion:The expression of Survivin in hilar cholangiocarcinoma is significantly higher than that in tissues near carcinoma,suggesting its correlation with tumor;Survivin plays an important role in the oncogenesis and metastasis of hilar cholangiocarcinoma;Survivin is statistically related with PTEN and nm23 and detected together may helpful for diagnosis and prognosis of hilar cholangiocarcinoma.
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Objective:To investigate the Survivin expression in the oncogenesis and progression of hepatocellular carcinoma and its clinical significant.Methods:The Survivin expression was determined in 50 cases of hepatocellular carcinoma and 34 cases of corresponding noncancerous tissue including 20 cases of paraneoplastic hepatitis tissue,8 cases of cirrhosis and 6 cases of normal hepatic tissue by RT-PCR,and the statistics analysis was followed.Results:The Survivin expression was detected in hepatocellular carcinoma.In contrast,no Survivin expression in corresponding noncancerous tissue was detected;The Survivin expression was significantly correlated with the histological differentiation,showing no correlation with age,tumor size,presence of portal vein thrombosis and intrahepatic metastasis.Conclusion:This experiment suggests that the Survivin may play an important role in the oncogenesis and progression of hepatocellular carcinoma.
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Objective To investigate the expression of Survivin in primary hepatocellular carcinoma(HCC)and its relationship with clinical pathological features and explore the role of Survivin in the development of HCC. Methods The expressions of Survivin were detected in 30 cases of HCC,20 cases of para-cancer tissue and 10 cases of normal liver tissue by flow cytometry (FCM) and immunohistochemistry (SP). The clinical data were analyzed retrospectively. Results ① The expression of Survivin in the HCC tissue was significantly higher than those in para-cancer tissue and normal liver tissue (P0.05);②The expression of Survivin was negative in normal tissue surrounding cancer; ③The expression of Survivin was independence of tumor size,location,capsule,AFP (P 0.05),while the expression of Survivin was related with endoscopic portal vein thrombosis formation (P0.05). Conclusion The hyperexpression of Survivin in tissue can reflex the biological behavior of HCC. The specificness of survivin protein expression make it to be new target of tumor diagnosis and treatment.
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Liver Cancer
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Objective To study the role of survivin expression in the development of cholangiocarcinoma.Methods Expression of survivin was detected by SP immunohistochemical technique in 33 cases of cholangiocarcinoma,28 cases of adjacent histologically noncancerous bile duct,and 5 cases of benign bile duct lesions.Results Survivin was expressed in 24 of 33 cholangiocarcinomas(72.7%).In contrast,no expression of survivin in adjacent histologically noncancerous bile ducts and benign bile duct lesions was detectable ( P 0.01).No correlation was found between expression of survivin and clinical features.Conclusion A high expression of survivin in cholangiocarcinoma suggests it may play an important role in the development of cholangiocarcinoma.
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Abstract Intrahepatic cholangiocarcinoma is a rare malignant biliary neoplasm that causes a poor prognosis even after curative hepatectomy. Liver metastasis is the major recurrence pattern of intrahepatic cholangiocarcinoma; therefore, the prevention of liver metastasis is a desirable objective. The aim of this study is to identify gene(s) related to liver metastasis of intrahepatic cholangiocarcinoma and to examine the inhibitory effects on metastasis of intrahepatic cholangiocarcinoma by controlling such gene(s). We collected 3 pairs of intrahepatic cholangiocarcinoma frozen samples, and 36 pairs (primary and metastatic lesions) of intrahepatic cholangiocarcinoma formalin‐fixed paraffin‐embedded samples, from patients who underwent surgical resection at hospitals related to the Kyushu Study Group of Liver Surgery between 2002 and 2016. We carried out cDNA microarray analyses and immunohistochemistry to identify candidate genes, and evaluated one of them as a therapeutic target using human cholangiocarcinoma cell lines. We identified 4 genes related to liver metastasis using cDNA microarray, and found that CXCL 12 was the only gene whose expression was significantly higher in liver metastasis than in primary intrahepatic cholangiocarcinoma by immunohistochemistry ( P = .003). In prognosis, patients in the high CXCL 12 group showed a significantly poor prognosis in disease‐free ( P < .0001) and overall survival ( P = .0004). By knockdown of CXCL 12 , we could significantly suppress the invasive and migratory capabilities of 2 human cholangiocarcinoma cell lines. Therefore, CXCL 12 might be associated with metastasis and poor prognosis in intrahepatic cholangiocarcinoma.
Intrahepatic Cholangiocarcinoma
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