An improved HPLC method for sucralfate in pharmaceutical dosage forms
0
Citation
0
Reference
20
Related Paper
Keywords:
Sucralfate
Cite
Amifostine
Cite
Citations (2)
Cite
Citations (0)
Simultaneous Analysis of Paracetamol and Flupirtine in Pharmaceutical Dosage Forms by RP-HPLC Method
A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of paracetamol and flupirtine in tablet dosage form. A YMC C18 5 mm column having 250 x 4.6 mm id in Isocratic mode with mobile phase containing 0.1 M potassium dihydrogen phosphate: methanol (70:30) was used. The flow rate was 0.8 ml/min and effluents were monitored at 271 nm. The method was validated for accuracy, precision, specificity, linearity and sensitivity. The retention times of paracetamol and flupirtine were 1.8 min and 3.0 min respectively. The calibration curves were linear over a concentration range from 81-243 µg/ml for paracetamol and over a concentration range from 25-75 µg/ml for flupirtine. Limit of detection (LOD) and limit of quantitation (LOQ) were 3.83 and 12.77 for paracetamol and 1.05 and 3.51 for flupirtine, respectively. The developed method was fast, accurate, precise and successfully applied to estimate the amount of paracetamol and flupirtine in bulk sample and tablet dosage form so it can be used for regular quality control of the drug.
Phosphate buffered saline
Cite
Citations (0)
Cite
Citations (9)
The process is from the penultimate intermediate namely 5-(5-chlorosulphonyl-2-ethoxy phenyl) -1-methyl-3-N-propyl-1,6-dihydro-7H-pyrasolo-(4,3-d)pyrimidin-7-one, which is herein will be referred to as chlorosulphonyl intermediate ( 21).This intermediate is condensed with N-methylpiperazine (2) in a solvent preferably of chlorinated hydrocarbon in presence of a trisubstituted amine or in presence of mixture of such amines (Figure 3) [2].
Cite
Citations (0)
A simple, economic, selective, precise and accurate High-Performance liquid Chromatographic method used for the estimation of Adenosine in bulk drug.The mobile phase used was of Mixture of Acetonitrile and water in the proportion 5:95 respectively.This Mobile phase was allowed to flow at rate of 0.8ml/min.And this was found to give a sharp peak of Adenosine at a retention time of 3.78 min.Analysis of HPLC for Adenosine was carried out at a wavelength of 256 nm.Linear regression analysis data for the Calibration curve showed a good linear relationship, in concentration range of 50-100ppm and regression coefficient 0.991.The linear regression equation was Y=71258× the developed method was employed with a high degree of precision and accuracy for the analysis of adenosine.The inter and intraday variation was less than 2%.The mean recovery of the drug was 99.39%.The proposed method is simple, fast, accurate, and reproducible hence, it can be applied for routine quality control analysis of Adenosine.
Cite
Citations (3)
The Chromatographic condition were successfully developed for the separation of Acyclovir and Hydrocortisone by using Agilent C8 column (25 cm x 4.6 mm i.d., 5 μ).A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Hydrocortisone and Acyclovir from pharmaceutical formulation.The method was carried out on a C8 (25 cm x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of Methanol: water (adjusted to pH 3.0 using ophosphoric acid) in the ratio of 80:20 v/v.The retention time of Hydricortusone and Acyclovir was 3.50 min and 6.00 min respectively with the flow rate of 1mL/ min.Eluents were detected at 254 nm.The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for Hydrocortisone and Acyclovir were R 2 =0.9995 and R 2 =0.9996 in the concentration range of 10-40µg.mL -1 , 20-80 µg.mL -1 respectively.The relative standard deviation for intra-day precision was lower than 2.0 %.The method was validated according to the ICH guidelines.The method was also found to be robust.The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability.
Cite
Citations (1)
Pharmaceutical Sciences
Cite
Citations (0)
Cite
Citations (0)
Amlodipine
Cite
Citations (0)