Influence of explant type, sucrose and IBA on in vitro growth of Acer platanoides L. 'Jirka'.
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Portulaca quadrifida (Portulacaceae) is an annual succulent herb having medicinal value and is consumed as a vegetable or salads in India. In the present study, leaf explants were inoculated on Murashige and Skoog’s (MS) medium fortified with sucrose (3%) and combinations of N6-benzyladenine (6-BA) and kinetin (KIN) individually and in combination with 1-naphtalene acetic acid (NAA). Rapid regeneration was observed in medium fortified with combinations of 6-BA (8 µM) and NAA (1 µM) which formed 19.40 ± 0.64 shoots with 100% response. Variation in sucrose concentrations (4-6%) was tried but it failed to increase the shoot number. When the optimized medium was fortified with different carbon sources viz. dextrose, glucose and maltose, they could not evoked better response and sucrose proved to be more effective for regeneration. Rooting of in vitro shoots was achieved in ½MS + sucrose (1%) + indole-3-butyric acid (IBA, 2 µM).
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Culture Laboratory, Horticulture Research Institute, Agricultural Research Center, during 2008/2009 to induce rooting on the in vitro regenerated micro-shoots of Polygala myrtifolia L. on MS medium.These shoots were supported in the culture using four gelling agents (agar at 7.0 g/l, gelrite at 2.0 g/l and agar+gelrite (at 3.5+1.0g/l, respectively), in addition to a filter paper bridge on a liquid MS medium.The micro-shoots were presoaked in different IBA concentrations (0.0, 0.5, 1.0 and 1.5 ppm) before inoculation on a growth regulator-free 3/4 MS medium.Results indicate that rooting % and total chlorophyll content increased significantly in agar + gelrite solidified medium, while gelrite significantly increased number of roots/plantlet.IBA at 1.0 ppm significantly increased rooting %, the number of roots/plantlet, root length, total chlorophyll, indoles and total soluble sugars %.The combined effect of the supporting type and IBA concentration exerted a significant effect.Rooting % increased to the significantly highest record when using agar + gelrite in a medium free of IBA.Therefore, from an economical point of view, it can be recommended in the rooting stage to use agar + gelrite (at 3.5+1.0g/l MS medium, respectively) without presoaking in IBA solutions to obtain the highest rooting %.
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