In vitro transfer of carbapenem resistant genes from Klebsiella pneumoniae to Escherichia coli J53
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Abstract:
Nocosomial pathogen Klebsiella pneumoniae is a gram - negative bacterium that carries multiple antimicrobial resistant genes. Conjugative method was used for investigating of gene transfer from clinical carbapenem-resistant K. pneumoniae isolates to a recipient E. coli J53 in vitro. Multiplex PCR & Real-time PCR was used for detection of transferable genes among these strains. Transconjugants showed resistance to multiple antibiotics due to the presence of ESBLs & AmpC -lactamase as well as carbapenemase encoding genes. This is the great concern in Vietnam because resistant E. coli may become part of the normal gut flora and thereby a notable source of infections among sick and healthy persons in healthcare settings and in the community.Keywords:
Carbapenem
Colistin
Klebsiella infections
Multi drug resistant
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Bacteriostatic experiment of twelve species of Chinese medicines and its compounds against escherichia coli K88 and hemoclatic escherichia coli had been made.The result showed that nine species of Chinese medicines had shown bacteriostasis against escherichia coli K88 and eleven species against hemoclastic escherichia coli. All compounds had shown bacteriostasis against escherichia coli Kgg and hemoclastic escherichia coli.The bacteriostatic effect of compuond 5 was superior to enrofloxacin's(1‰) against escherichia coli K88 (P0.05), and the bacteriostatic effect of compound 5 was superior to enrofloxacin's (1‰) against hemoclastic escherichia coli (P0.01 ).There was synergism between coptis root and enrofloxacin.
Escherichia
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Objective To investigate the prevalence and genotype of plasmid-mediated AmpC β-lactamases genes in Escherichia coli and Klebsiella pneumoniae strains isolated from Ningbo area.Methods After the detection for drug resistant phenotypes by K-B method,positive strains were subjected by polymerase chain reaction(PCR),then qualified through three-dimension test.Results From 2009 to 2011,the positive rates of plasmid-mediated AmpC genes were 4.4%(2/45),8.6%(3/35),13.3%(8/60),respectively.Meanwhile,the unique genotype was DHA for all positive strains in 2009 and 2010.In 2011,ACT-1 genotype was detected in 2 strains of Escherichia coli and 1 strain of Klebsiella pneumoniae.Conclusion The plasmid mediated AmpC genes in strains of Escherichia coli and Klebsiella pneumoniae clinically isolated in Ningbo area Escherichia coli and Klebsiella pneumoniae show an increasing tendency in recent years.
Klebsiella
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Objective To study the expression and resistance of plasmid-mediated AmpC gene in continuous isolates from Escherichia coli and Klebsiella pneumoniae. Methods The DHA and ATC-1 types of AmpC enzymes in continuous isolates from Escherichia coli and Klebsiella pneumoniae were detected by polymerase chain reaction(PCR). Results In 68 isolates Escherichia coli, there are 4 isolates, whose ATC-1 types were positive, and the DHA types were all negative. In 44 isolates Klebsiella pneumoniae, there are 4 isolates, whose DHA types were positive, and ATC-1 types were all negative. Conclusions Plasmid-mediated AmpC lactamase gene can be transferred to allogenic and xenogenic bacteria by conversion and conjugation. So they should be under careful surveillance.
Klebsiella
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목적: 내인성 내안구염은 드물지만 예후가 좋지 않은 질환으로 알려져 있다. 최근 내인성 내안구염에서 그람양성균 보다 그람음성균이 주요 원인균으로 나타나고 있는 것이 경험되고 있고 이에 대한 증례 보고들이 있어 내안구염의 주요 원인균과 진단, 그 예후에 대해 알아보고자 하였다. 방법: 2000년부터 2011년까지 총 3개 병원에서 내인성 내안구염이 총 23명의 환자에서 진단되었으며 이들은 환자의 임상증상과 징후 및 안과적 조사를 통해서 진단되었다. 환자의 안과적 수술 및 시술 시의 안구 검체 배양 및 혈액배양, 농배양 등을 통해 원인균을 확인하였으며 전체 환자에서 원발 감염의 원인 조사를 위한 복부전산화단층촬영이 시행되었다. 예후는 안과적 시력추적을 통해 확인하였다. ê²°ê³¼: 전체 23명의 환자 중에서 총 18명(78%)의 환자에서 혈액, 농, 눈 등에서 원인균이 동정되었다. 가장 흔한 원인균주는 Klebsiella pneumoniae로 14명(61%)의 환자에서 동정되었다. 복부전산화단층촬영에서 23명의 환자 중 14명(61%)의 환자에서 간농양 소견이 보였다. 치료에도 불구하고 23명의 환자 중 6명만이 시력의 호전을 보였으며 13명에서는 시력이 나빠지는 결과를 보였다. 그중 4명에서는 안구적출술을 시행하였다. ê²°ë¡ : 내인성 내안구염의 원인균으로 K. pneumoniae가 가장 흔하게 나타났으며 간농양이 가장 흔한 원발 감염으로 보인다. 따라서 내인성 내안구염으로 진단받은 환자에서 원발감염에 대한 조사로서 복부전산화단층촬영 등을 시행하고 간농양을 확인하고 필요 시 배농이 필요할 것으로 생각되었다. 내인성 내안구염의 빠른 경과와 나쁜 예후를 볼 때 빠른 진단 및 안과적 처치가 필요하며 항생제의 선택 시 K. pneumoniae 등의 그람음성균에 대한 치료 약제가 선택되어야 할 것으로 생각된다. 중심 단어: 내인성 내안구염; Klebsiella pneumoniae
Klebsiella
Klebsiella infections
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Treatment of infections with Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases (ESBLs) is challenging due to the coexistence of multiple resistance mechanisms and the hypervirulent variant. Therefore, new targets or more effective treatment options aimed at ESBL-producing Klebsiella pneumoniae are urgently needed.Here, we collected ESBL-producing and non-ESBL Klebsiella pneumoniae isolates and studied their differences from a proteomic point of view.We revealed treA, wza, gnd, rmlA, rmlC, rmlD, galE, aceE, and sucD as important virulence-related proteins in ESBL-producing Klebsiella pneumoniae, distinct from those in non-ESBL strains.Our findings provide plausible anti-virulence targets and suggest new therapeutic avenues against ESBL-producing Klebsiella pneumoniae.
Klebsiella
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Objective To establish the proteomic diagnostic model for Klebsiella pneumoniae strains producing extended spectrum beta-lactamases(ESBLs) and search for a rapid method to identify them. Methods Klebsiella pneumoniae proteins were detected by the PBS Ⅱ/C protein fingerprint spectrometer and Au protein chip.The differentially expressed proteins of Klebsiella pneumoniae ESBLs positive and negative strains were analyzed and an artificial neural network(ANN) model of Klebsiella pneumoniae ESBLs producing strains was established and validated. Results Totally,47 protein peaks were detected for the moleculars with mass-to-charge ratio(m/z) within the range of 2,000-20,000.The peaks with m/z of 5,985.5,8,361.6 and 9,169.8 were screened by ANN as the protein spectrum model of Klebsiella pneumoniae ESBLs producing strains.The validation result of the model indicated that the sensitivity was 96.88% and the specificity was 93.62%. Conclusions The protein spectrum model established by this study can identify Klebsiella pneumoniae ESBLs producing strains accurately and rapidly.It is worth further studying.
Klebsiella
Protein chip
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A multiplex PCR was described to simultaneously detect mcr-1 and frequently occurring carbapenem-resistant genes including blaKPC, blaNDM, blaIMP, and blaOXA-48-like in a single reaction. The PCR product sizes of these 4 carbapenem-resistant genes were 232 bp, 438 bp, 621 bp, and 798 bp for blaIMP, blaOXA-48-like, blaNDM, and blaKPC, respectively, whereas mcr-1 revealed 1126 bp of PCR product. This protocol accurately detected those resistant genes in agreement with the reference strains, 127 local carbapenem-resistant Enterobacteriaceae, 8 mcr-1 carrying Enterobacteriaceae, and 62 carbapenem-susceptible Enterobacteriaceae. This method will be useful for laboratory application and surveillance of carbapenem and/or colistin-resistant bacteria.
Colistin
Carbapenem
Carbapenem-resistant enterobacteriaceae
Multiplex
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ABSTRACT We aimed to examine the effects of resistance mechanisms on several resistance phenotypes among carbapenem-resistant Klebsiella pneumoniae isolates with borderline carbapenem MICs. We compared carbapenemase-negative K. pneumoniae with carbapenemase-producing K. pneumoniae (CPKP) isolates with similar MICs. CPKP isolates exhibited a marked inoculum effect and were more resistant to the bactericidal effect of meropenem. This suggests that MIC measurements alone may not be sufficient in predicting the therapeutic efficacy of carbapenems against CPKP.
Carbapenem
Ertapenem
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Background Carbapenems are frequently used to treat infections due to extended-spectrum β-lactamase-producing Klebsiella pneumoniae . Thus, the emergence of infections due to carbapenem-resistant K . pneumoniae (CRKp) is a major public health concern.
Carbapenem
Klebsiella infections
Klebsiella
Carbapenem-resistant enterobacteriaceae
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