Protective effects ofCoptis chinensisinflorescence extract and linarin against carbon tetrachloride-induced damage in HepG2 cells through the MAPK/Keap1-Nrf2 pathway
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Abstract:
Coptis chinensis inflorescence is traditionally used as tea and has been popular in the local market. C. chinensis inflorescence extract (CE) exhibits protective effects against carbon tetrachloride (CCl4)-induced damage, but the underlying mechanism remains unclear. The main chemicals of CE were detected, purified, and identified in this study. CE and linarin could reverse changes in cell viability, decrease alanine aminotransferase and aspartate transaminase levels, and reduce reactive oxygen species (ROS) generation induced by CCl4 in HepG2 cells. CE and linarin could also phosphorylate mitogen-activated protein kinases (MAPKs) and up-regulate Kelth-like ECH-associated protein (Keap1). The pathways of MAPKs and Keap1 lead to the separation of Keap1 and nuclear factor (erythroid-derived 2)-like 2 (Nrf2). Free Nrf2 transferred to the nucleus and enhanced the expression of phase II detoxification enzymes. This study provides a scientific basis for the use of C. chinensis inflorescence, which exhibits a hepatoprotective function, as a supplement in the food industry.Keywords:
Coptis chinensis
Objective The paper reported some kinds of traditional Chinese medicine's inhibition to Enteropathogenic E.coli,O86:H2,like Coptis chinensis and Scutellaria baicalensis Georgi,etc,including water extractions and alcohol extractions.Methods Choose Coptis chinensis 、Scutellaria baicalensis Georgi 、Fructus Forsythiae 、Allium sativum L、Rheum palmatum L.、Berberis vernae Schneid、Floslonicerae、Radix Pulsatillae、Sophora flavesens、Portulace oleracea L.,put these medicines into boiling water and ethanol,which have been obtained by successive extractions with ethanol,and tested these liquids' MICs and MBC.by broth dilution technique.Results Rheum palmatum L.、Floslonicerae and Fructus Forsythiae are almost no activities against Enteropathogenic E.coli,and the remaining seven kinds of Chinese medicine show a different antibacterial strength,Ccoptis chinensis、Scutellaria baicalensis Georg、Allium sativum L.are good at antibacterial activity.The MICs of Allium sativum and Coptis chinensis with extractions with ethanol are1.96 mg/ml against 2 strains of Enteropathogenic E.coli O86:H2,the antibacterial activities of Radix Pulsatillae、Berberis vernae Schneid and Sophora flavesens are second.Conclusion Coptis chinensis、Scutellaria baicalensis Georg、Allium sativum L.are bactericidal against Enteropathogenic E.coli,suggesting that Chinese herbal medicines may represent a new source of agents designed for inhibition Enteropathogenic E.coli in eradicated therapy.
Coptis chinensis
Coptis
Scutellaria
Houttuynia cordata
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Objiective To explore the inhibitory effects of 13 kinds of Chinese herbal medicines on N.gonorrhoeae in vitro. Methods The thirteen kinds of Chinese herbal medicines mainly inclnded Eugenia caryophyllata Thunb,Coptis chinensis Franch,Polygonum cuspidatum. et Zucc,Rhus chinensis Mill,Agrimonia pilosa Ledeb,Pulsatilla chinensis(Bge.)Regel and so on. The ethnal extrac was made from each of thirteen kinds of Chinese herbal medicines and diluted to different concentrations . Then their bacteriostasis were observed and minimum concentration (MIC) of inhibition of N.gonorrhoeae was determined. Results The MIC of Coptis chinensis Franch was 6.25mg/ml; The MIC of Pericarpium granati and Polygonum cuspidatum. et Zucc was 12.5mg/ml; The MIC of Phellodendron chinese Schneid 、Eugenia caryophyllata Thunb and Rhus chinensis Mill was 25mg/ml.The MIC of Sophrra flavescens Ait was 100mg/ml.Conclusions Coptis chinensis Franch,Pericarpium granati, Polygonum cuspidatum. et Zucc ,Phellodendron chinese Schneid,Eugenia caryophyllata Thunb and Rhus chinensis Mill have strong inhibitory effects on N.gonorrhoeae in vitro.The Coptis Chinensis Franch has the strongest effect on N.gonorrhoeae in 13 kids of Chinese herbal medicines.
Coptis chinensis
Coptis
Polygonum
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Bacteriostasis in vitro of 10 species of herb,isatis root,Coptis chinensis,Cyrtomium fortunei,Houttuynia cordata,Forsythia suspense,Pueraria lobata,Sophora flaveseens,Phellodendron amurense,Atractylodes macrocephala and Astragalus membranaceus,were tested with their extractions in different gradient concentration.In vitro inhibition on Salmonella was observed by determining minimal inhibitory concentration(MIC) with tube 2-fold dilution method.The results showed that Cyrtomium fortunei and Coptis chinensis had the best inhibition effect on Salmonella with the mean MIC of 7.8 g/L being the minimum among the herbs,and isatis root,Houttuynia cordata,Forsythia suspense,Pueraria lobata,Sophora flaveseens,Atractylodes macrocephala and Astragalus membranaceus also had good inhibition effect with MIC of 15.6～31.3 g/L,but Phellodendron amurense had weak effect with MIC of 62.5 g/L.
Houttuynia cordata
Coptis chinensis
Sophora flavescens
Astragalus
Forsythia
Sophora
Glycyrrhizin
Atractylodes
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Objective: By investigating the regulation of prescription-herbs on treating lung cancer in ancient literatures,to provide the new thoughts for the clinical diagnosis and treatment. Methods: The prescription-herbs were summarized from the ancient literatures by using methods of literatures research combined with statistic analysis. Results: Four factors which relected the treatment characteristics were extracted. F1: Radix aconiti, Dried ginger, Coptis chinensis, et al; F2: Tangerine peel,Balloonlower, et al; F3: Ginger, Glycyrrhiza, et al; F4: Lepidium seed, Jujube date; Four prescriptions were summarized, C1:Tangerine peel, Balloonlower, radix ginseng, et al; C2: Lepidium seed, Jujube date, et al; C3: Cinnamon and Evodia rutaecarpa;C4: Radix auckladiae and Areca catechu, et al. Conclusions: Lung cancer was a disease of fundamental deiciency and incidental excess, involving with lung, spleen and kidney. Deiciency of healthy qi, phlegm-dampness, qi stagnation, blood stasis and the others were the main characteristics of pathogenesis of lung cancer. Adopting diferent methods according to the characteristics of pathogenesis, including reinforcing the healthy qi, regulating qi, activating blood circulation, dispelling Phlegm and so on.
Coptis chinensis
Phlegm
Blood stasis
Radix (gastropod)
Coptis
Glycyrrhiza
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Purpose To screen traditional Chinese medicines using powerful antibacterial activity against methicillin-resisistant Staphylococcus aureu(MRSA).Methods The Chinese medicines with powerful antibacterial activity against MRSA were selected from 50 traditional Chinese medicines by Kirby-Bauer.The MICs of selected Chinese medicines with powerful antibacterial activity against MRSA were performed by agar disk.Synergetic antibacterial test of each two of the selected 5 Chinese medicines against MRSA were carried out by trace multi-well culture plates.Results 35 Chinese medicines had inhibitory effects on the strain of MRSA with varied extent in 50 Chinese medicines,among which were Coptis chinensis,Cortex Phellodendri,Rhubarb,Radix Stellariae and granatum.Furthermore,FIC index is 0.5 when Coptis chinensis and Cortex Phellodendri were in combined use.Conclusion Most of the selected traditional Chinese medicines have certain inhibitory effect on MRSA,in which Coptis chinensis,Cortex Phellodendri,Rhubarb,Radix Stellariae and granatum have powerful antibacterial activity against MRSA,and the combined use of Coptis chinensis and Cortex Phellodendri has the strongest inhibitory effect on MRSA because of synergistic effect,and it is the better choice in anti-MRSA Chinese medicines.
Coptis chinensis
Coptis
Radix (gastropod)
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Objective:To investigate antibacterial property of clinical common used 9 kinds of single herb to diabetes foot's common bacteria.Methods:Selecting of Coptis chinensis,Scutelaria baicalensis,Phellodendron chinensis,Flos lonicerae japonieae,Prunella angelica,Angelica sinensis,Ligusticum chuanxiong,Sophora flavescens,Fructus Kochiae granule,using AGAR dilution method,and determinating of minimum inhibitory concentration of each Chinese herbal medicine.Results:Eight kinds of single herbs had different degrees of antibacterial activity to the common bacteria of diabetes foot,except Fructus Kochiae MIC value was higher than 500.00mg/mL.The effects of Coptis chinensis,Scutelaria baicalensis,Prunella asiatica were better than others,as they mean inhibitory concentration of 20 Staphylococcus aureus were 8.59,37.50,17.97mg/mL.Conclusion:Coptis chinensis,Scutelaria baicalensis,Prunella asiatica to diabetes foot common bacteria had different degrees of antimicrobial function,the higher the concentration the better the effect.
Coptis chinensis
Sophora flavescens
Flos
Angelica sinensis
Prunella vulgaris
Coptis
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AbstractThe cytotoxic potential of 160 crude extracts from 80 kinds of Korean medicinal herbs was evaluated with cultured human hepatocellular carcinoma cells, Hep3B and HepG2. Aqueous extracts of Caesalpinia sappan, Coptis chinenis, and Lepidium apetalum, and methanol extracts of Angelica gigas, Areca catechu, C. sappan, C. chinensis, Ephedra sinica, L. apetalum, Magnolia obovata, Peucedanum praeruptorum, and Rheum palmatum, were active against Hep3B. Aqueous extracts of C. sappan, C. chinensis, L. apetalum, and P. praeruptorum, and methanol extracts of A. catechu, C. sappan, Cinnamomum cassia, C. chinensis, L. apetalum, M. obovata, P. praeruptorum, and U. pumila, exhibited cytotoxic activity against HepG2.
Coptis chinensis
Catechu
Carthamus
Cinnamomum
Houttuynia cordata
Ranunculaceae
Sophora flavescens
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Preventive and Therapeutic Effects of Traditional Medicines on Diabetic Complications: Sophora flavescens, Pueraria lobata, Coptis chinensis, Cirsium...
Sophora flavescens
Coptis chinensis
Lobata
Flos
Coptis
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Objective The purpose of this study was to compare the inhibiting effects of four Chinese medicinal herb decoctions(such as Coptis chinensis Franch) on Enterococcus Faecals in the infected root canals.Methods A total of 84 single-rooted extracted human teeth were used in this study.The teeth were randomly divided into four groups(Coptis chinensis Franch decoction group,Schisandra Chinensis decoction group,Chinese rhubarb decoction group and Frucrus forsythia decoction group) and three control groups(FC group,negative control group,and positive control group).Every group contained 12 teeth.The E.faecalis infected root canal models were manufactured after normal root canal preparation and sterilization.The teeth of negative control group was not infected by bacteria,and placed in 0.9% sodium chloride injection.The other teeth were infected by E.faecalis and treated with different Chinese medical herbs decoction separately.Transmittances of each group were measured after 3 days and 7 days.Results There was no difference in the following comparisons,Coptis chinensis Franch decoction group(3 d) and FC group(3 d) comparison,Frucrus Forsythia decoction group(3 d) and positive control group(3 d) comparison,Frucrus Forsythia decoction group(3 d) and negative control group(7d) comparison,Chinese rhubarb decoction group(3 d),Coptis chinensis Franch decoction group(7 d) and FC group(7 d) comparison,Frucrus Forsythia(7 d) and positive control group(7 d) comparison,(P0.05).There were statistical significance in the other groups.(P0.05).Conclusions In the four Chinese medical herbs mentioned above,Coptis chinensis Franch,Schisandra Chinensis and Chinese rhubarb had inhibition effects on E.faecalis at different levels.Among the four Chinese medical herbs,the most powerful one was Coptis chinensis Franch,that has the same inhibition effects as FC.Frucrus Forsythia has no inhibition effects on E.faecalis.
Decoction
Coptis chinensis
Enterococcus faecalis
Coptis
Forsythia
Negative control
Positive control
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Objective: The effective components of Fructus Aurantii, Pulsatilla chinensis, Schisandra chinensis, Sanguisorba officinalis, Scutellaria baicalensis, Terminalia chebula, Rheum officinale, Punica granatum, Fructus Mume, Cortex Phellodendri and Coptis chinensis were extracted, and the minimum inhibitory concentrations of components from these Traditional Chinese Medicines were analyzed in vitro against Staphylococcus chromogenes, S. xylosus, S. pseudintermedius, Yokenella regensburgei, Aeromonas hydrophila and Klebsiella pneumoniae, so as to lay a theoretical foundation for the application and development of Traditional Chinese Medicine in clinical treatment. Methods: The effective components of Traditional Chinese Medicines were extracted by alcohol extraction, and the minimal inhibitory concentration of single Traditional Chinese Medicine was determined by tube dilution method. Results: S. chinensis, P. granatum, F. Mume and T. chebula showed the most significant bacteriostatic effects on S. xylosus (MICs were all 62.50 mg/mL); S. chinensis presented the most significant antibacterial effect on S. chromogenes (MIC was 31.25 mg/mL); S. chinensis, P. granatum and T. chebula had the most significant bacteriostatic effects on S. pseudointermedia (MICs were all 31.25 mg/mL); S. chinensis, S. officinalis, T. chebula, R. officinale, F. Mume and C. chinensis had the most significant bacteriostatic effects on Y. regensburgei (MICs were all 15.60 mg/mL); The inhibitory effect of S. officinalis on A. hydrophila was the most significant (MIC was 1.95 mg/mL); F. Mume had the most significant antibacterial effect on K. pneumoniae (MIC was 0.98 mg/mL). Conclusion: S. chinensis, F. mume and T. chebula et al. have good antibacterial effects, thus providing basis for the clinical application of these Traditional Chinese Medicines.
Terminalia chebula
Coptis chinensis
Punica
Magnolol
Houttuynia cordata
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