Characterization of the Cry1Ah resistance in Asian corn Borer and its cross-resistance to other Bacillus thuringiensis toxins
31
Citation
56
Reference
10
Related Paper
Citation Trend
Abstract:
Transgenic crops producing insecticidal proteins are effective to manage lepidopteran pests. Development of insect-resistance is the major threat to Bacillus thuringiensis (Bt) crops such as Cry1Ah-Maize. Laboratory selection with Bt-Cry1Ah toxin incorporated in artificial diet, during 48 generations of Asian corn borer (ACB) Ostrinia furnacalis produced 200-fold resistance. This resistant colony ACB-AhR readily consumed and survived on Cry1Ah-expressing Bt-maize. Cross-resistance analysis showed high cross-resistance to Cry1F (464-fold), moderate cross-resistance to Cry1Ab (28.38-fold), Cry1Ac (22.11-fold) and no cross-resistance to Cry1Ie toxin. This ACB-AhR cross-resistant phenotype is different from ACB-Cry1Fa resistant population that showed no cross resistance to Cry1Ah, suggesting that different mechanisms of resistance were selected in these two populations. Bioassays of reciprocal F1 crosses-progeny suggested autosomal inheritance of Cry1Ah resistance with no maternal effects. The dominance of resistance increased as concentration decreased. In Cry1Ah-maize tissues the progeny of reciprocal F1 crosses behaved as functionally recessive. Progenies analysis from backcrosses (F1 × resistant strain) suggested polygenic contribution to Cry1Ah- resistance in ACB-AhR. The use of multiple toxins is an imperative factor for delaying evolution of resistance to Cry1Ah-corn in ACB. However, the fact that ACB-AhR showed cross resistance to Cry1Fa indicates that selection of toxins for pyramided plants should be carefully done.Keywords:
Bacillus thuringiensis
European corn borer
Toxicity of Bacillus thuringiensis Spo − Cr + Mutants for the European Corn Borer Ostrinia nubilalis
Inclusion bodies isolated from Spo − Cr + mutants of Bacillus thuringiensis were toxic for larvae of the European corn borer. Probit analysis revealed comparable toxicity between wild-type crystals (isolated from B. thuringiensis subsp. kurstaki ) and crystals produced from two spore-free mutants of the same subspecies. Death of the larvae was due to starvation, presumably through δ-endotoxin-induced gut paralysis. Inclusion bodies pretreated with α-chymotrypsinogen were equally as toxic as native crystals for the insect larvae.
Ostrinia
Bacillus thuringiensis
European corn borer
Genetically modified maize
Cite
Citations (10)
Bacillus thuringiensis
European corn borer
Genetically modified maize
Cite
Citations (0)
Bacillus thuringiensis
European corn borer
Genetically modified maize
Cite
Citations (0)
Bacillus thuringiensis
Bacillaceae
Cite
Citations (272)
Bacillus thuringiensis
European corn borer
Genetically modified maize
Western corn rootworm
Cite
Citations (2)
Ostrinia
Bacillus thuringiensis
European corn borer
Genetically modified maize
Biopesticide
Cry1Ac
Cite
Citations (11)
CORN BORER, 1998: Significant infestations of European corn borer (ECB) in corn can result in yield loss due to stalk breakage and reduced ear size.Bt corn has become a top choice for many growers to ensure against economic loss due to large populations of the pest.Comparative performance of Bt corn events was evaluated at the Rosemount Experiment Station near Rosemount, Minnesota.Twelve Bt hybrids, representing five different events, and ten of their non-Bt isolines were replicated four times in a RCB design.Test hybrids were planted on 12 May in eight-row plots (30ft long, 30-inch row spacing).In each plot, 15 consecutive plants were infested at the V6 stage with an average of 40 neonate larvae in grits deposited in the whorl on 2 Jul.The natural infestation during second generation was judged adequate and supplemental infestation was cancelled.Damage evaluations included: number and length of tunnels, number of overwintering larvae, and shank and ear damage on 27 Oct.These data were analyzed using the SAS ANOVA procedure; means from this procedure can be found in the following table with mean separations based on LSD tests of transformed data.
Bacillus thuringiensis
European corn borer
Genetically modified maize
Cite
Citations (0)
The field of plant genetic engineering has arisen from the laboratory and into the market place as a technology to provide farmers and consumers with improved crops. 1996 marks a turning point as the first genetically engineered crops to control agronomically important pests are registered for commercial sale. In most cases it has taken over a decade to develop commercially viable products. This book serves both as an update of current technologies that have been proven successful for engineering insect tolerant crops and an overview of new technologies that are being pursued for the development of new genetically engineered crops in the future. The book includes an introductory chapter on the world wide importance of insect problems in crops and the advantages of genetically engineered crops over traditional breeding; reviews insect control principles that are being develpoed for genetically engineered crops; and provides an overview of many new areas that wil lead to new insect control agents in the next decade.
Bacillus thuringiensis
European corn borer
Genetically modified maize
Cite
Citations (10)
The challenge of Bacillus thuringiensis, K. van Frankenhuyzen diversity of Bacillus thuringiensis toxins and genes, D. Lereclus et al domain-function studies on Bacillus thuringiensis crystal proteins - a genetic approach, B. Visser et al transgenic bacteria, viruses, algae and other micro-organisms as Bacillus thuringiensis delivery systems, W. Gelrnter and G.E. Schwab the engineering of plants to express thuringiensis delta-endotoxins, S. Ely control of lepidopteran pests with Bacillus thuringiensis, A. Navon use of Bacillus thuringiensis isrealensis against mosquitoes and blackflies, N. Becker and J. Margalit control of coleopteran pests by Bacillus thuringiensis, B. Keller and G.A. Langenbruch Bacillus thuringiensis in the environment - ecology and risk assessment, M.P. Meadows resistance to Bacillus thuringiensis and resistance management, P.G. Marrone and S.C. MacIntosh the use of Bacillus thuringiensis in developing countries, H.S. Salama and O.N. Morris production of Bacillus thuringiensis insecticides for experimental and commercial uses, K. Bernhard and R. Utz.
Bacillus thuringiensis
Biopesticide
Bacillus (shape)
Bacillaceae
Cite
Citations (426)
Bacillus thuringiensis
European corn borer
Ostrinia
Nutrient agar
Cite
Citations (22)