"'Carnation Nou No.1', a carnation [Dianthus caryophyllus] breeding line resistant to bacterial wilt (Burkholderia caryophylli)."
1
Citation
0
Reference
16
Related Paper
Citation Trend
Keywords:
Dianthus
Cite
Cite
Citations (0)
これまでに得られた萎凋細菌病抵抗性に関与するQTLの近傍に存在するDNAマーカーの実際の萎凋細菌病抵抗性育種における有用性について調査した．これまで浸根接種により選抜してきた抵抗性戻し交雑系統のマーカーの有無を調査した結果，主働抵抗性遺伝子に連鎖したマーカーであるSTS-WG44は全ての系統が保有していた．一方，作用の小さい2つのQTL近傍のマーカーであるOQ12とSTS-WB66は戻し交雑を進めた系統で保有する割合が低下した．このことからSTS-WG44が抵抗性個体を選抜する上で有効であることが明らかになった．実際の育種集団を用いて，STS-WG44の有無と発病率を調査した結果，STS-WG44の有無による発病率の差は62.6％と大きく，発病率が20％以下の強抵抗性系統のほとんどはSTS-WG44を保有していた．本研究により，カーネーションの萎凋細菌病抵抗性育種においてSTS-WG44を選抜マーカーとして用いることで，強度の抵抗性を有する系統を含む平均発病率の低い集団へ絞込みが可能であり，DNAマーカーによるマーカー選抜育種が可能であることを明らかにした．
Dianthus
Marker-Assisted Selection
Cite
Citations (4)
The four spotted-wilt-resistant tomato types, Lycopersicon pimpinellifolium and L. esculentum varieties Rey de los Tempranos, Pearl Harbour, and Manzana, were each crossed with the susceptible variety Potentate, and also crossed with each other in all possible combinations. The F 1 and F 2 plants, when inoculated with 10 strains of tomato spotted wilt virus, were resistant to five groups of these strains.
Lycopersicon
Cite
Citations (91)
Bacterial wilt
Ralstonia solanacearum
Cite
Citations (5)
Tomato cultivation was set back in Chongqing(China) due to severe incidence of bacterial wilt caused byRalstoniasolanacearum.Hence,studies were initiated toidentify the pathogen,screen resistant resources,and breed hybrids with resistance to thedisease.68 isolates of pathogen of tomato bacterial wilt were obtained during 2000-2003.The morphological characteristics,culturalcharacteristics,biochemical properties and pathogenicity of the isolates indicated that they wereRalstonia solanacearum,race 1,andbiovar Ⅲ.Studied of methods of seedlings inoculation revealed that cutting and dipping method was the best one,compared with root-stabbing andleaf-clipping.4 tomatolines,which had highresistance to bacterial wilt,had been screened out from184 genotypes.Among12 hybrids that crossed fromthe 4 resistances,4012 showed higher yield,good qualities,and promising resistance.
Bacterial wilt
Ralstonia solanacearum
Cite
Citations (2)
Bacterial wilt caused by Ralstonia solanacearum is one of the major diseases in Solanum species including cultivated Eggplant (Solanum melongena L.). Bacterial wilt (BW) disease management in eggplant is difficult due to high survival rate of pathogen in soil and chemical application is not eco-friendly. The best way to avoid bacterial wilt in eggplant is using disease-resistant varieties. However, only a limited number of bacterial wilt resistant varieties are available and, there is a necessity to identify and/or develop new resistant varieties. In the current study, wild Solanum species, and eggplant cultivated varieties were evaluated against Ralstonia solanacearum, and disease incidence was recorded. The cultivated varieties IIHR-108, Pusa Purple Long and Rampur Local were identified as susceptible, whereas, IIHR-7 and CARI-1 were identified as resistant to bacterial wilt. These resistant wild and cultivated varieties can be used as a root-stock in bacterialwilt disease resistant breeding programmes.
Bacterial wilt
Ralstonia solanacearum
Melongena
Wilt disease
Cite
Citations (5)
Resistance of carnation to Fusarium wilt (Fusarium oxysporum f. sp. dianthi) is of major importance for breeding new cultivars. New germplasm and combination between ornamental traits and high resistance to Fusaruim wilt is a main achievement in the selection of this crop. The investigation of the response of parental forms and new selected hybrid material is a main step in breeding programs. Interspecific hybridization of carnation allows increasing the diversity in germplasm. Five spray carnation cultivars and two breeding lines of D. caryophyllus f. spray Hort. (Krassina, Regina, Fea, Naslada, Barbara, L-169 and L-230) used as a mother plant and seven Dianthus species used as a father plant (D. carthusianorum, D. gratianopolitanus, D. sequieri, D. knappii, D. silvestris, D. plumarius and D. chinensis) were evaluated for their resistance to Fusarium wilt after artificial inoculation with the causal pathogen of the disease. The resistance of wild Dianthus species is much lower compared to this of spray carnation cultivars. Hybridisation was performed after castration and compulsory, forced pollination with pollen collected from different species under isolation. All tested breeding lines showed a very high level of resistance to F. oxysporum f. sp. dianthi. The level of resistance in F1 and BC1 is variable. Clones of individual plants in some crosses showed a very high level of resistance, reaching complete resistance. Interspecific hybridization gives an opportunity to select plant forms combining variable ornamental traits with high level of resistance to Fusarium wilt.
Interspecific hybrids
Cite
Citations (0)
Abstract Safflower ( Carthamus tinctorius L.) is a multipurpose oilseed crop. Fusarium wilt ( Fusarium oxysporum f.sp. carthami ) is the major damaging disease in safflower. In the present investigation, Fusarium wilt resistance was introgressed from two wild species, Carthamus oxyacantha and Carthamus palaestinus, into susceptible cultivated species through interspecific hybridization. Inheritance of wilt resistance indicated single dominant gene control. Eight simple‐sequence‐repeat (SSR) markers each in (‘Nira’ × C. oxyacantha ) and (‘Nira’ × C. palaestinus ) were found to be linked to wilt resistance. Marker‐assisted selection for wilt resistance was performed using these markers in F 3 –F 7 generations of both crosses. Six wilt resistant interspecific lines evaluated for 2 years under nondisease conditions have recorded 9%–29% higher seed yield than the high yielding cultivar, ‘A1’. The wilt resistant lines would serve as new sources of resistance to wilt in safflower. The SSR markers linked to wilt resistance would be useful for precise selection of wilt resistance at seedling stage in large segregating populations without attempting screening in artificially inoculated conditions and pyramiding of wilt resistant genes from wild into a common background.
Carthamus
Marker-Assisted Selection
Wilt disease
Cite
Citations (11)
Fusarium wilt, caused by Fusarium oxysporum F. sp.phaseoli caused growers to abandon the most popular climbing bean cultivar, Umubano (G2333) in Rwanda.The present objective was to determine the nature of inheritance of fusarium wilt resistance and recommend a breeding strategy to introduce resistance into susceptible cultivars.Two cultivars, vuninkingi (G685) and flora were donors of resistance to fusarium wilt whereas G2333 was highly susceptible.Injured root tips of 10-day old seedlings of the parents, progenies of F 1 and F 2 (G2333 × G685) and (G2333 × Flora), backcrosses F 2 (G685 × Flora) were inoculated with 10 6 conidia ml -1 of Rwandan isolate of F. oxysporum F. sp phaseoli (FOP-RW2) in a glasshouse.The disease severity was rated 28 days later using the CIAT scale of 1 -9, where 1 -3 represent resistant, 4 -6 tolerant and 7 -9 susceptible reactions.The chi-square analysis was performed to determine the Mendelian segregation ratios of resistant and susceptibility among the inoculated progenies.The F 1 and the backcross progenies to the resistant parents segregated in the ratio of 1:0 as did the F 2 population (G685 × Flora).The F 2 progenies segregated in the ratio of 3:1.The backcross progenies to the susceptible parent segregated in the ratio of 1:1.Resistance to fusarium wilt is conditioned by a single highly heritable major dominant gene.The resistance can be achieved by backcross breeding.
Flora
Cite
Citations (2,188)
Bacterial wilt caused by Ralstonia solanacearum is sporadically causing damage to pepper in Korea. We put efforts in analyzing the genetics of resistance to bacterial wilt and in subsequent breeding for resistance by selection. Two Korean cultivars, Subi and Chilsung, were crossed with a resistant source, MC-4, which was kindly provided by Lopes in Brazil. In addition, four breeding lines bred for resistance to Phytophthora blight were crossed with another resistance source, PBC631, which we received from AVRDC. F 2 and backcross populations of the crosses were developed and tested by inoculation by dipping the roots of the seedlings before transplanting 25 days after seeding. Segregation in the F 2 and backcross populations did not fit any mode of simple Mendelian inheritance, but appeared inheriting in a quantitative mode with relatively low heritability. Selection was practiced in the subsequent F 3 , BC 1 F 2 , F 4 , and BC 1 F 3 generations and a few resistant selections were obtained.
Bacterial wilt
Ralstonia solanacearum
Transplanting
Breeding program
Cite
Citations (0)