EP-1048: Phantom Tumour Phenomenon in Nasopharyngeal Carcinoma Patients after Radiotherapy
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Objective To explore the correlation between radiotherapy and the serumal level of endogenous nitric oxide (NO) in patient with nasopharyngeal carcinoma (NPC). Methods The levels of NO were detected by nitrate reductase method in sera from 20 patients with NPC at the time points before radiotherapy, immediately after radiotherapy, and one year after radiotherapy. 27 healthy adults were taken as control; their levels of NO were also detected. Results ① The levels of NO in NPC patient before radiotherapy was significantly higher than that in of control group (P0.01). ② The levels of NO in NPC patients immediately after radiotherapy were significantly lower that those before radiotherapy (P0.01). ③ The levels of NO in NPC patients one year after radiotherapy were higher than those in control group and NPC patients immediately after radiotherapy (P0.01), but much lower than those in NPC patients before radiotherapy (P0.05). Conclusion NO may play an important role in the carcinogenesis and development of NPC at molecular level. Concentration monitoring of serumal NO maybe helpful to evaluate the state and prognosis of NPC patients.
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Objective:There were variations of EBV genome f rom different areas and they may affe ct the biologic function of EBV(such as LMP1).EBV-BARF0was highly detected in th e patients with nasopharyngeal carc inoma (NPC)and its variations have not been rep orted.This study was designed to determine the sequence and variation of EBV-BARF0gene in the patieats with NPC from Guangdong area.Methods :PCR was used to amplify the EBV-BARF0gene in 20patients with NPC and the production s were sequenced on the ABI377.Results:Comparing with standard B95-8,there were four loci with variances i ncluding:160473(G→T),160545(C→T),160701(C→A),160707(G→C)of sequences and 2(Ala→Ser),26(Leu→Phe),78(Arg→Ser),80(Ala→Pro)of amino acid in 20patients with NPC.Conclusion:Since the BARF0gene of EBV is expressed in all nasopharyngeal carcinoma c ell line and biopsies,but it is not expressed or less frequenly in lymphoma tissues and cell lines,the authors firstly reported the variation of EBV-BARF0which compared with B95-8in the patieats with NPC from Guangdong area.These su ggest that the gene may play an important role in the carcinogenesis and development of nasopharyngeal carcinoma .
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To study the impact of radiotherapy on middle ear pressure in nasopharyngeal carcinoma(NPC)patients.First diagnostic NPC patients(n=60)with normal hearing in language zone and A type graph of tympanic pressure before radiotherapy were randomly divided into two groups.The first group received local treatment of nasal cavity and nasopharynx radiotherapy.The second group received radiotherapy alone.Tympanic pressures of the first and the second group was measured at the end of radiotherapy.At the end of radiotherapy,there was significant difference in the measurement of tympanic pressure(z=-5.159,P0.005)between the first group and the second group.[Conclusions]More negative pressure values of the middle ear was mainly associated with organic obstruction of eustachian tubes at the end of radiotherapy in nasopharyngeal carcinoma patients.Local treatments of nasal cavity and nasopharynx have a good effect.
Eustachian tube
Tympanic cavity
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<div>Abstract<p>In this study, we aimed to use the combined detection of multiple antibodies against Epstein–Barr virus (EBV) antigens to develop a model for screening and diagnosis of nasopharyngeal carcinoma (NPC). Samples of 300 nasopharyngeal carcinoma patients and 494 controls, including 294 healthy subjects (HC), 99 non-nasopharyngeal carcinoma cancer patients (NNPC), and 101 patients with benign nasopharyngeal lesions (BNL), were incubated with the EUROLINE Anti-EBV Profile 2, and band intensities were used to establish a risk prediction model. The nasopharyngeal carcinoma risk probability analysis based on the panel of VCAgp125 IgA, EBNA-1 IgA, EA-D IgA, EBNA-1 IgG, EAD IgG, and VCAp19 IgG displayed the best performance. When using 26.1% as the cutoff point in ROC analysis, the AUC value and sensitivity/specificity were 0.951 and 90.7%/86.2%, respectively, in nasopharyngeal carcinoma and all controls. In nasopharyngeal carcinoma and controls without the non-nasopharyngeal carcinoma and BNL groups, the AUC value and sensitivity/specificity were 0.957 and 90.7%/88.1%, respectively. The diagnostic specificity and sensitivity of the EUROLINE Anti-EBV Profile 2 assay for both nasopharyngeal carcinoma and early-stage nasopharyngeal carcinoma were higher than that of mono-antibody detection by immune-enzymatic assay and real-time PCR (EBV DNA). In the VCA-IgA–negative group, 82.6% of nasopharyngeal carcinoma patients showed high probability for nasopharyngeal carcinoma, and the negative predictive value was 97.1%. In the VCA-IgA–positive group, 73.3% of healthy subjects showed low probability. The positive predictive value reached 98.2% in this group. The nasopharyngeal carcinoma risk probability value determined by the EUROLINE Anti-EBV Profile 2 might be a suitable tool for nasopharyngeal carcinoma screening. <i>Cancer Prev Res; 10(9); 542–50. ©2017 AACR</i>.</p></div>
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<div>Abstract<p>In this study, we aimed to use the combined detection of multiple antibodies against Epstein–Barr virus (EBV) antigens to develop a model for screening and diagnosis of nasopharyngeal carcinoma (NPC). Samples of 300 nasopharyngeal carcinoma patients and 494 controls, including 294 healthy subjects (HC), 99 non-nasopharyngeal carcinoma cancer patients (NNPC), and 101 patients with benign nasopharyngeal lesions (BNL), were incubated with the EUROLINE Anti-EBV Profile 2, and band intensities were used to establish a risk prediction model. The nasopharyngeal carcinoma risk probability analysis based on the panel of VCAgp125 IgA, EBNA-1 IgA, EA-D IgA, EBNA-1 IgG, EAD IgG, and VCAp19 IgG displayed the best performance. When using 26.1% as the cutoff point in ROC analysis, the AUC value and sensitivity/specificity were 0.951 and 90.7%/86.2%, respectively, in nasopharyngeal carcinoma and all controls. In nasopharyngeal carcinoma and controls without the non-nasopharyngeal carcinoma and BNL groups, the AUC value and sensitivity/specificity were 0.957 and 90.7%/88.1%, respectively. The diagnostic specificity and sensitivity of the EUROLINE Anti-EBV Profile 2 assay for both nasopharyngeal carcinoma and early-stage nasopharyngeal carcinoma were higher than that of mono-antibody detection by immune-enzymatic assay and real-time PCR (EBV DNA). In the VCA-IgA–negative group, 82.6% of nasopharyngeal carcinoma patients showed high probability for nasopharyngeal carcinoma, and the negative predictive value was 97.1%. In the VCA-IgA–positive group, 73.3% of healthy subjects showed low probability. The positive predictive value reached 98.2% in this group. The nasopharyngeal carcinoma risk probability value determined by the EUROLINE Anti-EBV Profile 2 might be a suitable tool for nasopharyngeal carcinoma screening. <i>Cancer Prev Res; 10(9); 542–50. ©2017 AACR</i>.</p></div>
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Objective To explore correlation between Epstern-Barr virus and angiogenesis of patients with nasopharyngeal carcinoma.Methods Epstern-Barr virus DNA of 54 patients with nasopharyngeal carcinoma was measured by PCR and their serum vascular endothelial growth factor(serum VEGF) level was also detected by ELISA,and these data was compared and analyzed with serum vascular endothelial growth factor level of 40 health control.Results Serum vascular endothelial growth factor level of 54 patients with nasopharyngeal carcinoma(143.86±19.49) pg/mL was very higher than that of 40 health control(51.23±12.66) pg/mL,(P0.01);Serum vascular endothelial growth factor level of 24 EBV positive patients with nasopharyngeal carcinoma(195.44±19.69) pg/mL was remarkable higher than that of 30 EBV negative patients with nasopharyngeal carcinoma(154.58±15.23 pg/mL),(P0.01).Conclusion Serum vascular endothelial growth factor level changed in patients with nasopharyngeal carcinoma may be as a new tumor mark for determining disease advancing about nasopharyngeal carcinoma.
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Nasopharyngeal carcinoma (nasopharyngeal carcinoma) is one of the most common tumor diseases at present. Due to its special clinicopathological characteristics and strong sensitivity to radiotherapy, it is easy to relapse and even cause distant metastasis after treatment. At present, the screening and diagnosis of nasopharyngeal carcinoma mainly relies on image detection. This detection method shows low sensitivity in the screening and clinical staging of nasopharyngeal carcinoma, so the effect is not ideal. The purpose of this paper is to study the significance of epstein-barr virus DNA concentration detection in the screening of nasopharyngeal carcinoma and clinical staging diagnosis, so as to provide theoretical support for the diagnosis of nasopharyngeal carcinoma. This article first to EB virus, and discusses the specific concept of nasopharyngeal carcinoma (NPC), then simply discusses the EB virus and development of the relationship between nasopharyngeal carcinoma, and the studies of the relationship between the simple illustration, finally analyzed with related experiments EB virus DNA testing in the value of screening and clinical staging diagnosis of nasopharyngeal carcinoma (NPC). The experimental results showed that the basic age composition of the three groups of people in the study was relatively consistent. The average age was 49.6 years old, and the majority of them were males, accounting for 85.4% of the total number of patients. The positive values of vca-iga antibody and epstein-barr virus DNA in different groups were the highest in the nasopharyngeal carcinoma group, with concentrations of 91.6% and 52.1%, respectively. Compared with the traditional detection methods, the detection of EB virus DNA concentration has been significantly improved in the diagnosis speed and effect of nasopharyngeal carcinoma, with the diagnosis speed increased by about 17% and the diagnosis efficiency increased by about 30%.
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OBJECTIVE:To explore the clinical significance of quantitative detection of Epstein-Barr virus infectious status in nasopharyngeal carcinoma tissues.METHODS:The carcinoma tissues and adjacent tissues from 30 patients with poorly differentiated nasopharyngeal squamous cell carcinoma were prepared simultaneously,and nasopharyngeal tissues from 15 healthy subjects were used as the control.The EBV DNA copies in the samples were tested by using rea1-time quantitative polymerase chain reaction(PCR).RESULTS:The infectiou rates of EBV were 73.3%(11/15)in nasopharyngeal tissues of healthy subjects(mean EBV DNA copies of 6.5×102 μg-1 DNA),100%(30/30)in carcinoma tissues and 76.7%(23/30)in adjacent tissues of nasopharyngeal carcinoma patients(mean EBV DNA copies of 6.7×105 μg-1 DNA and 1.3×105 μg-1 DNA)respectively.There was no significant diference in the infectious rates and levels of EBV between the adjacent carcinoma tissues of nasopharyngeal carcinoma patients and nasopharyngeal tissues of healthy subjects(P0.05).The infectious level of EBV was significantly higher in carcinoma tissues than in adjacent tissues,and significantly higher in adjacent tissues than in normal nasopharyngeal tissues of nasopharyngeal carcinoma patients(P0.01).CONCLUSIONS:EBV latent infection is a common phenomenon in nasopharyngeal tissues of both nasopharyngeal carcinoma patients and healthy persons.The enhancement of EBV infection plays an important role during carcinogenesis of nasopharyngeal carcinoma patients,which sheds further light on the occurrence of EBV with nasopharyngeal carcinoma.
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The effect of radiotherapy on antibodies to EBV-induced membrane antigens (MA) was studied in 6 patients with Burkitt's lymphoma (BL), 16 with nasopharyngeal carcinoma (NPC), and 18 controls receiving radiotherapy for other malignant tumors. The tests were performed on sera taken shortly before radiotherapy, about 2 months after radiotherapy, and, in some cases of nasopharyngeal carcinoma and Burkitt's lymphoma, 4–20 months after radiotherapy as well. About 2 months after radiotherapy, there was an increase in anti-MA antibody levels in the BL group and in the NPC group as well. This increase persisted in the group of NPC patients followed during a longer time after radiotherapy. With one exception, a maxillary carcinoma, there was no increase in the control group. Possible implications of these findings are discussed.
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had higher sensitivity to irradiation treatment. Conclusion Our results suggest that patients with nasopharyngeal carcinoma have elevated ebv-miR-BART7 expression levels in the primary and recurrent nasopharyngeal carcinoma tissues. Higher expression of ebv-miRBART7 increased the sensitivity of nasopharyngeal carcinoma cells to irradiation treatment. Further investigation with regard to the clinical value of ebv-miR-BART7 to aid therapeutic response is warranted in order to evaluate the potential use of ebv-miR-BART7 as a molecular biomarker in monitoring patients with nasopharyngeal carcinoma.
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