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    Effect of Electrode-Surface Area on Electricity Generation in Microbial Fuel Cells
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    Abstract:
    Abstract. Microbial fuel cells (MFCs) are prospective biodevices for the purification and energy recovery from organic wastewater. In this study we examined the effect of surface areas of anode and cathode on electrical power output in MFCs. Hydrogen, produced by anaerobic bacteria, is one of the possible mediators for electricity generation in MFCs. A reactor consisted of an anode, air cathode, and proton-exchange membrane was filled with a phosphate buffer without inoculation of bacteria, and hydrogen gas as a fuel for electricity generation was dissolved in the buffer. The cathode contained Pt catalyst, but the anode did not it. When the surface areas of anode and cathode were changed from 6 to 25 cm2, the electrical power outputs were proportional to the cathode area but not to the anode area. To examine the electricity generation by bacterial, an artificial wastewater containing peptone and beef extract was filled in the reactor, and activated sludge was inoculated as the seed bacteria. The reactor was operated at room temperature over two months, and the electricity generation was monitored. The electrical power output by bacteria with regard to the change in the surface areas of anode and cathode showed a similar tendency to those with hydrogen in the absence of bacteria, i.e., it was dependent on only the cathode area. These results suggest that the power output of the MFC can be increased by enlarging the cathode area.
    We report a multi-anode paper-based microbial fuel cell (MFC) capable of generating a power density of 28.4 μW/cm 2 . This MFC features (i) flexible multilayered carbon anodes for bacterial attachment and (ii) paper reservoirs for holding the anolyte and catholyte for an extended period of time. By using the multi-anode MFC, the power and current densities increased by 5x and 3x, respectively, compared to a single anode one. The paper-based MFC is expected to be a simple and easy-to-use power source for single-use diagnostic biosensors because even sewage or soiled water in a puddle can become an excellent source for operating MFCs and harvesting electricity through bacterial metabolism.
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    Microbial fuel cell consisting two main components which are anode and cathode materials. In the microbial fuel cell, both anode and cathode compartments are separated with a separator. Anode generates the protons and electrons while cathode converts protons into water with the presence electrons and oxygen. During the Microbial fuel cell operation, the performance of anode is very crucial due to it provides the protons and electrons. Hence, the high efficiency microbial fuel cell is very related with the high anode performance. This work addressed to the enrichment process of electroactive bacteria (EAB) in anode of microbial fuel cell. In this work, some parameters such as current generations, , and pH changes were used to assess the enrichment process of EAB was reached. In addition, the presence of EAB on the anode surface was identified based on the morphology of anode surface. The removal of COD and the pH value were determined by using the American public health analysis method and pH tester, respectively. The morphology of anode surface was analysed by using a scanning electron microscope. Whereas, current generation was tested by using a mustimeter. The removal of COD and final pH were obtained 71.4 % and 5.7, respectively. The optimum current generation was observed 0.19 mA. The surface morphology of anode before enriched with microbes was clear surface, while after enriched with microbes was attached by microbes. The removal of COD, pH changes, current generation and morphology of anode surface could be used to assess the EAB in the anode compartment. Keywords: Microbial fuel cell; anode; cathode; electroactive bacteria; pH changes.
    The enhancement of microbial activity and electrocatalysis through the design of new anode materials is essential to develop microbial fuel cells (MFCs) with longer lifetimes and higher output. In this research, a novel anode material, graphene/Fe3 O4 (G/Fe3 O4 ) composite, has been designed for Shewanella-inoculated MFCs. Because the Shewanella species could bind to Fe3 O4 with high affinity and their growth could be supported by Fe3 O4 , the bacterial cells attached quickly onto the anode surface and their long-term activity improved. As a result, MFCs with reduced startup time and improved stability were obtained. Additionally, the introduction of graphene not only provided a large surface area for bacterial attachment, but also offered high electrical conductivity to facilitate extracellular electron transfer (EET). The results showed that the current and power densities of a G/Fe3 O4 anode were much higher than those of each individual component as an anode.
    Shewanella
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    A novel single cathode chamber and multiple anode chamber microbial fuel cell design(MAC-MFC)was developed by incorporating multiple anode chambers into a single unit and its performance was checked.During 60 days of operation,performance of MAC-MFC was assessed and compared with standard single anode/cathode chamber microbial fuel cell(SC-MFC).The tests showed that MAC-MFC generated stable and higher power outputs compared with SC-MFC and each anode chamber contributed efficiently.Further,MAC-MFCs were incorporated with different wastewaters in different anode chambers and their behavior in MFC performance was observed.MAC-MFC efficiently treated multiple wastewaters simultaneously at low cost and small space,which claims its candidature for future possible scale-up applications.
    Single chamber
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    The characteristic of anode plays an important role in the performance of the microbial fuel cell (MFC). Thus, carbon nanotube (CN), flexible graphite (FG) and activated carbon (AC) were used as anode material in this study, and the performances of three MFCs (CN-MFC, FG-MFC and AC-MFC) were studied. The results show that CN is a kind of suitable material to be used as anode in the MFC. The maximal power densities of CN-MFC, FG-MFC and AC-MFC are 402,354 and 274 mW/m2, respectively. The CN-MFC shows a higher power density and coulombic efficiency compared with FG-MFC and AC-MFC. The CN-anode can reduce the internal resistance obviously. The internal resistances of CN-MFC, AC-MFC and FG-MFC are 263, 301 and 381 omega, respectively. The protein contents on the CN-anode, AC-anode and FG-anode are 149, 132 and 92 microg/cm2 after stable operation, and there is a positive relation between the protein content and internal resistance. The conductivity of the three types of MFCs from high to low was FG-MFC, CN-MFC and AC-MFC, which was accordant with the ohmic resistance. The stable times of CN-MFC, FG-MFC and AC-MFC, which were needed to measure the internal resistances, were 1800, 1200 and 300 s respectively.
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    Carbon fibers
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    Nano-CeO2 was used to modify the carbon felt anode in microbial fuel cell (MFC). The MFC with the modified anode obtained the higher closed circuit voltage resulting from the lower anode potential, the higher maximum power density (2.94 W m−2), and the lower internal resistance (77.1 Ω). Cyclic voltammetry (CV) results implied that the bioelectrochemical activity of exoelectrogens was promoted by nano-CeO2. Electrochemical impedance spectroscopy (EIS) results revealed that the anodic charge transfer resistance of the MFC decreased with modified anode. This study demonstrates that the nano-CeO2 can be an effective anodic catalyst for enhancing the power generation of MFC.
    Internal resistance
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