Mir-16 and 21 Deregulation Are Linked to Anti-Apoptotic Bcl-2 Gene Expression in Polycythemia Vera
Natália de Souza NunesRaquel Tognon-RibeiroLívia Gonzaga MouraSimone KashimaDimas Tadeu CovasElizabeth Xisto SoutoMaria Aparecida ZanichelliBelinda Pinto SimõesAna Maria de SouzaFabíola Attié de Castro
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Bone marrow derived progenitor cells participate in the repair of injured vessels. The lungs of individuals with emphysema have reduced alveolar capillary density and increased endothelial apoptosis. We hypothesized that circulating levels of endothelial and hematopoietic progenitor cells would be reduced in this group of patients.The goal of this study was to measure circulating levels of endothelial progenitor cells (EPCs) and hematopoietic progenitor cells (HPCs) in subjects with COPD and to determine if progenitor levels correlated with disease severity and the presence of emphysema.Peripheral blood mononuclear cells were isolated from 61 patients with COPD and 32 control subjects. Levels of EPCs (CD45(dim) CD34+) and HPCs (CD45(+) CD34(+) VEGF-R2(+)) were quantified using multi-parameter flow cytometry. Progenitor cell function was assessed using cell culture assays. All subjects were evaluated with spirometry and CT scanning.HPC levels were reduced in subjects with COPD compared to controls, whereas circulating EPC levels were similar between the two groups. HPC levels correlated with severity of obstruction and were lowest in subjects with severe emphysema. These associations remained after correction for factors known to affect progenitor cell levels including age, smoking status, the use of statin medications and the presence of coronary artery disease. The ability of mononuclear cells to form endothelial cell colony forming units (EC-CFU) was also reduced in subjects with COPD.HPC levels are reduced in subjects with COPD and correlate with emphysema phenotype and severity of obstruction. Reduction of HPCs may disrupt maintenance of the capillary endothelium, thereby contributing to the pathogenesis of COPD.
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This study was undertaken to determine the effect of short-course high-dose methylprednisolone (HDMP) treatment on peripheral blood (PB) CD34+ progenitor cells during remission induction treatment in 11 children with newly diagnosed acute leukemia (7 with ALL, 4 with AML) whose bone marrow (BM) cells expressed fewer than 5% CD34 at the time of diagnosis. All children who had no infection were given HDMP as a single daily oral dose of 30 mg/kg for the first four days of induction therapy. The number of CD34+ progenitor cells were determined by flow cytometry before and after four days of HDMP treatment. While the number of PB blast cells significantly decreased after only a four-day course of HDMP treatment, the number of PB CD34+ progenitor cells increased in all patients. In addition, after four days of HDMP treatment polymorphonuclear leukocytes (PMN) and mononuclear cells (MNC) increased significantly (p < 0.05). We suggest that the potential beneficial effects of HDMP in the induction treatment of acute leukemia may occur partly by the stimulation of PB CD34+ hematopoietic progenitor cells in a short period of time.
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Polycythemia vera is an acquired myeloproliferative disorder that causes overproduction of all three hematopoietic cell lines, most prominently the red blood cells. The most common of the chronic myeloproliferative disorders, polycythemia vera occurs in about 0,5-2 per 100,000 persons. A slight overall male predominance has been observed, but females predominate within the reproductive age range. In this case report, We aimed to emphasize that cytogenetic analysis recently is important in the diagnosis of polycythemia vera.
Myeloproliferative neoplasm
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Objective Through the clinical analysis of 30 cases of polycythemia vera, we explore basic clinical characteristics of this disease, and reduce the misdiagnosis of polycythemia vera, so as to improve the treatment effect. Methods This study analyzed clinical data of polycythemia vera in September 2008 to October 2013. Results In the 30 cases of patients with polycythemia vera,misdiagnosis were 10 cases, accounting for 33.3%; in the patients with good treatment, 8 cases of remission, 18 patients improved significantly, effective treatment accounted for 86.7%. Conclusion The early symptoms of polycythemia vera were not obvious. The misdiagnosis rate was high; in order to reduce the misdiagnosis, the therapeutic effect must be improved.
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Abstract Background: Despite treatment advances, systemic lupus erythematosus (SLE) patients frequently experience disease flares, which can lead to organ damage and premature death. Therefore, assessing disease activity in SLE patients is crucial for adjusting treatment and preventing further organ damage. The aim of this study was to investigate progenitor cells and circulating endothelial cells levels in relation to SLE activity and accumulate organ damage. Methodos: A case-control study was conducted. CD34 + CD45 low/- progenitor cells, CD34 + CD45 low/- CD133 + progenitor, Endothelial Progenitor cells (EPC) and Circulating Endothelial cells (CEC) levels in peripheral blood were assessed by flow cytometry. Results: Thirty-two SLE patients and 28 matched controls were included. SLE patients had lower levels of CD34 + CD45 low/- progenitor cells (p=0.001), CD34 + CD45 low/- CD133 + progenitor cells (p=0.016), EPC (p=0.018) and CEC (p<0.001) compared to controls. In addition, cell subpopulations studied correlate with SLE activity biomarkers. CD34 + CD45 low/- progenitor cells showed a moderate negative correlation with levels of both C3 and C4. We also found significantly higher levels of CD34 + CD45 low/- progenitor cells, CD34 + CD45 low/- CD133 + progenitor cells, EPC and CEC in patients with SLE with SDI scores ≥1 versus those without organ damage (p=0.0073, p=0.018, p=0.018 and p=0.020, respectively). Conclusion: We found that CD34 + CD45 low/- progenitor cells, CD34 + CD45 low/- CD133 + progenitor cells, CPE and CEC were significantly reduced in patients with SLE as well as associated with disease activity and organ damage. Our observations suggest that CD34 + CD45 low/- progenitor cells could serve as a potential biomarker for disease activity and organ damage in SLE patients. It should be confirmed in a prospective study.
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A group of 54 patients with the original diagnosis of polycythemia vera were subjected to cytogenetic examination. Six (17.6%) of the 34 cases examined in the period of the advanced phase of the polycythemia vera had a chromosomal change. Thirteen (65%) of the 20 patients undergoing the cytogenetic examination in the period when the polycythemia vera turned into another myeloproliferative disease showed chromosomal aberration. This suggests a relationship between the number of chromosomal changes and the transformation of the disease. No connection between the cytogenetic changes and myelosuppressive cures could be confirmed in our material. The chromosomal change 20q- considered to be the most frequent kind in the polycythemia vera was not discovered until in patients with the polycythemia vera transformed into a different myeloproliferative disease.
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