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    Transformation of heterolous DNA in Bacillus subtilis v1
    Kristoffer Bach FalkenbergCristina Hernández‐RollánMaja RennigAndreas Birk BertelsenMorten H. H. Nørholm
    B. subtilis is a gram-positive bacteria used by both academia and industry as a protein production workhorse. This is due to its' excellent fermentation properties, high production titers, and capacity to secrete proteins into the extracellular medium. This protocol describes transformation of B. subtilis by natural competence. The method utilizes the natural stress-induced competence of B. subtilis to take up heterologous DNA. The protocol requires the cells to be grown for a specific amount of time in starvation media (SM). The protocol is adapted from Vojcic, L., Despotovic, D., Martinez, R., Maurer, K., & Schwaneberg, U. (2012). An efficient transformation method for Bacillus subtilis DB104. Applied Microbiology and Biotechnology, 94(2), 487–493. https://doi.org/10.1007/s00253-012-3987-2.
    Heterologous
    Citations (0)
    Cytochrones of Bacillus subtilis
    敬三郎 三木
    Citations (0)
    BACILLUS | Bacillus Subtilis
    Elsevier eBooks (1999)
    Michael K. Dahl
    Bacillus (shape)
    Citations (2)
    Solution Structure of MciZ from Bacillus subtilis
    Journal of Back and Musculoskeletal Rehabilitation (2015)
    Patrícia CastellenMaurício L. SforçaAna Carolina de Mattos ZeriFrederico J. Gueiros‐Filho
    Citations (0)
    Bacillus subtilis YABJ
    Janet L. SmithShipra Das SinhaPekka RappuStephan LangePekka Mäntsälä
    Citations (0)
    YpmB protein from Bacillus subtilis
    J. OsipiukN. MaltsevaI. DementievaS. MoyA. Joachimiak
    Citations (0)
    YDCE protein from Bacillus subtilis
    Arhonda GogosHongmei MuFabiana BahnaCamila GomezLawrence Shapiro
    Citations (0)
    Structure of YTNJ from Bacillus subtilis
    Jason GormanL. ShapiroS.K. Burley
    Citations (0)
    NATURE OF THE PRIMARY ACTION OF THE AUTOLYSIN OF BACILLUS SUBTILIS
    Journal of Bacteriology (1956)
    Michio NomuraJunko Hosoda
    Autolysin
    Primary (astronomy)
    Bacillus (shape)
    Citations (39)
    Protein expression in Bacillus subtilis v1
    Kristoffer Bach FalkenbergCristina Hernández‐RollánMaja RennigAndreas Birk BertelsenMorten H. H. Nørholm
    B. subtilis is a gram-positive bacteria used by both academia and industry as a protein production workhorse. This is due to its' their excellent fermentation properties, high production titers, and capacity to secrete proteins into the extracellular medium. This protocol describes how to express proteins in B. subtilis. The protocol is developed using KO7-S, although it might also work for other strains as well. The method is adapted from Rasmussen, M. D.; Bjoernvad, M. E.; Diers, I. Pectate Lyase Fusion for Expression and Secretion of Polypeptides. WO 00/75344, 2000 and Jensen, K.; Østergaard, P. R.; Wilting, R.; Lassen, S. F. Identification and Characterization of a Bacterial Glutamic Peptidase. BMC Biochem.2010, 11 (1), 47. https://doi.org/10.1186/1471-2091-11-47.
    Wilting
    Citations (0)