Baseline HBsAg and HBcrAg Titers Allow Peginterferon Based “Precision Medicine” in Hbeag-Negative Chronic Hepatitis B
M PeignouxMarie-George LapalusNathalie BoyerCorinne CastelnauNathalie GiuilyM. PouteauTarik AsselahPatrick Marcellin
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Baseline (sea)
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1000 pairs of maternal and cord blood samples were collected simultaneously at the time of delivery. 23 (2.3%) of the maternal samples were positive for HBsAg by enzyme-linked immunosorbent assay. HBeAg was detected in 11 (47%) of the 23 HBsAg positive mothers and anti-HBeAg was detected in another 5 samples. HBsAg and HBeAg were detected in 7 (30%) of the 23 cord blood samples from HBsAg-positive mothers, and anti-HBeAg was detected in one of these samples. At follow-up (6-18 months), antigenaemia had persisted in 17 (85%) of the 20 HBsAg-positive mothers and in 9 (45%) of 20 babies born to HBsAg-positive mothers. Seven of the 10 babies (70%) born to mothers positive for both HBsAg and HBeAg had persistent HBsAg in their blood, in contrast to 2 of the 10 babies (20%) born to mothers positive for HBsAg only. However, none of these mothers or their babies were found to have anti-HBeAg at follow-up. We conclude that the presence of HBeAg in mothers' blood enhances vertical transmission of hepatitis B virus infection to their babies.
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Objective: To study the inhibitory effect of Gantai capsules (肝泰胶囊, GTC) on replication and expression of HBsAg and HBeAg. Methods: The 2.2.15 cell line was chosen as a in vitro cell culture system, and by means of radioimmunoassay, the hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in cell cultured medium were examined. Results: HBsAg, HBeAg values of 2.2.15 cells treated by GTC (200 μg/ml-1200 μg/ml) were lower than those of the control. And this inhibitory effect was in a dose dependent manner under experimental condition, GTC had no cytotoxicity. Conclusion: GTC could inhibit HBV replication and expression.
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Objective: To study the inhibitory effect of FFYC tablets (FFYC) on expression of HBeAg and HBsAg. Methods: The 2.2.15 cell line was cultured with cell cultured medium including different concentration of FFYC. By means of radioimmunoassay, the hepatitis B surface antigen ( HBsAg) and hepatitis B e antigen (HBeAg) were examined. Results: The max cytotoxicity concentration of FFYC was 600g/mL. Based on this concentration, FFYC was diluted and was added in cell culture medium. The results showed that HBeAg, HBsAg values of 2.2.15 cells treated by FFYC were lower than those in the control. Conclusion: FFYC could inhibit HBV replication and expression.
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The vertical transmission of the hepatitis B surface antigen (HBsAG) from HBsAg carrier mothers to their infants and children were studied in 42 mother-infant pairs, 27 siblings aged under 5 years and 34 fathers of these families. Thirteen out of 42 (30.9%) infants born from these mothers became HBsAg carriers at 3 to 6 months of age. Vertical transmission to their infants (76.5%) and other siblings (88.9%) occurred only in those mothers with e-antigen (HBeAg) positive but none in HBsAg carrier mothers without HBeAg. Most of the HBsAg carrier babies (70.5%) and their siblings (77.7%) born from the carrier mothers with HBeAg positive also had HBeAg in their sera. Good correlation of the presence of HBeAg and higher titer of HBsAg was found both in HBsAg carrier mothers and in their off-springs. This study clearly shows that HBsAg carrier Thai mothers with HBeAg transmitted hepatitis B virus vertically to their infants and children more readily than do the HBsAg carrier mothers without HBeAg.
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Objective:To study genetic transmission from the parents with HBsAg to their children.Methods:Five serum HBV markers(HBsAg,anti HBs,HBeAg,anti HBe and anti HBc) and serum free HBV DNA were detected in 234 individuals from 117 families.Rusults:There was a significant difference in total infection rate of HBV,HBsAg,HBeAg,HBV DNA between the children of males with HBsAg and those of males with no HBsAg.The positive rate of HBV serum markers combination of father and their children was 52 4% in 63 families that father and children were all infected with HBsAg.HBV DNA mainly distributed in the combination that father and their children were all with HBsAg,there was a significant difference between that and other combinations.HBV DNA rised in accord with HBeAg.The childrens HBeAg and HBV DNA were all positive,who born after occurrence of HBeAg.HBV DNA of father with high titer HBeAg rised in accordence with there childrens.Conclusion:It showed that hepatitis B virus,can be transmitted by inheritance between the fathers and their children.
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Hepatitis B
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To constructed the shRNA expressing vectors targeting HBsAg gene and HBeAg gene of HBV, Pgs1, Pgs2, Pgs3 and psiHBV4, psiHBV6 in order to prove their inhibitation on the HBV antigen expression in outlive HepG2. 2. 15 cells.PTZ was used as negative control, both the shRNA expressing vectors targeting HBsAg and HBEAg gene of HBV was together transfected into HepG-2. 2. 15 cells with different combinations, and detected the expression liquid and the cultivated supernatant with MEIA after 24 h.The group transfecting psiHbv4 and PgS2, psiHBV4 and PgS3 could significantly inhibit HBsAg and HBeAg expression compared with control group (P < 0.05) in the cell disruption liquid and the cultivated supernatant. But the group transfecting psiHBV5 and PgS1, psiHBV6 and PgS3 cannot significantly inhibit HBeAg expression (P > 0.05) in the cell disruption liquid.The shRNA expressing vectors targeting HBsAg and HBeAg gene of HBV psiHBV4 and PgS2, psiHBV4 and HBeS3 could significantly inhibit the antigen expression of HBV than only one.
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Objective To study the effect of antisense phosphorothioate oligodeoxynucleotides (S-as ODN) of various regions of the HBV genome on the expression of HBsAg and HBeAg in the HepG22215 cell line. Method The expression levels of HBsAg and HBeAg in the HepG22215 cell line were determined by ELISA. According to gene structure of HBV, 5 S-as ODNs were designed and synthesized. The effect of different ASODNs on the expression of HBsAg and HBeAg was analyzed. Results This confirmed the characteristics of HepG22215 which could produce HBsAg and HBeAg. All 5 S-as ODNs could specifically inhibit the production of HBsAg and HBeAg in HepG22215 cells. A combination of drug and ASODN could enhance the inhibition. Conclusion These results suggest that S-as ODN may be a potential new therapy for anti-HBV. The inhibition showed sequence-specific, dose-dependent and drug-cooperative effects.
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OBJECTIVE To investigate the active parts of the extracts of the core of Litchi and their effect on the HBV. METHODS HepG 2.215 cell culture system was used for study of the expressions of HBsAg and HBeAg with A、B、C、D、E、F of the extract components from the core of litchi. RESULTS All extracts A、B、C、D、E 、F (200,100 mg·L -1) of the core of litchi. Were shown to inhibit the expression of HBsAg and HBeAg in HepG 2.2.15 cells, among which the extract E was the most effective, at 200 mg·L -1 in the medium, HBsAg expression was inhibited by 50%, and HBeAg by 20% in the third day of the experiment, and in the 9 th day, the HBsAg was inhibited by 90.9%, the HBeAg by 84.3%(P 0.01, compared to the control group). CONCLUSION The extracts of the core of litchi are very effective in inhibition of HBV in vitro.
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Objective To observe the effect of the serum containing Liuyueqing(LYQ)on the expression of HBsAg and HBeAg in HepG2.2.15 cells.Methods The toxicity of the serum containing LYQ on HepG2.2.15 cells was detected by means of MTT.The inhibitory effects of the serum containing LYQ on the excreation of HBsAg and HBeAg in HePG 2.2.15 cells were evaluated with method of enzyme linked immunosorbent assay(ELISA).Results The dose which the serum containing LYQ could inhibit 50% HepG2.2.15 cells was 11.56 g.(kg.d)-1.The dose which the serum containing LYQ could inhibit 50% HBsAg was 0.178g.(kg.d)-1,and its treatment index(TI)on HBsAg was 64.94.The inhibitory effect of the serum containing LYQ on HBeAg was not obvious.Conclusion The serum containing LYQ can inhibit HBV significantly in vitro,and it is a low toxic drug.
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MTT assay
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