Identification and mapping of MLIW30, a novel powdery mildew resistance gene derived from wild emmer wheat
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Blumeria graminis
Common wheat
Bulked segregant analysis
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Blumeria graminis
Common wheat
Candidate gene
Bulked segregant analysis
Cite
Citations (0)
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Blumeria graminis
Common wheat
Bulked segregant analysis
Candidate gene
Cite
Citations (0)
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Blumeria graminis
Candidate gene
Bulked segregant analysis
Common wheat
Cite
Citations (0)
Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a destructive wheat disease. Although it can be easily overcome by deployment of resistance genes, the resistance is often quickly compromised by pathogen virulence. Thus, exploration and characterization of new resistance genes is always ongoing. Line NJ3946 derived from a cross of einkorn wheat accessions TA2032 and M389 showed resistance to powdery mildew. Inheritance analysis of an F2 population derived from a cross of NJ3946 and M389 suggested that the resistance was conferred by a dominant allele. With polymorphic markers identified through bulked segregant analysis (BSA), this gene was mapped to a novel locus on chromosome 3A, and was designated as PmNJ3946. Bulked segregant RNA-seq analysis (BSR-seq) was conducted to obtain more closely linked markers, which allowed delimitation of the PMNJ3946 locus to a 0.9 cM interval covering a physical distance of less than 1 Mb. PMNJ3946 was flanked by Xwgrc5153 and SNP-derived marker CHS21_3A008915069, and co-segregated with SNP-derived markers CHS21_3A008939814 and CHS21_3A008943175. The PmNJ3946 discovery expands the diversity of powdery mildew resistance genes and is useful for wheat breeding.
Bulked segregant analysis
Blumeria graminis
Common wheat
Cite
Citations (2)
Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat(Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated Ml WE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of Ml WE4 was constructed, and Ml WE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes Ml WE4, Pm36 and Ml3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of Ml WE4, Pm36 and Ml3D232.
Blumeria graminis
Bulked segregant analysis
Genetic linkage
Common wheat
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Citations (1)
Common wheat
RNA-Seq
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Citations (18)
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Plant Pathogens
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Citations (0)
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Blumeria graminis
Common wheat
Bulked segregant analysis
Candidate gene
Cite
Citations (0)
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Blumeria graminis
Common wheat
Bulked segregant analysis
Candidate gene
Cite
Citations (0)
Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive disease leading to huge yield losses in production. Host resistance can greatly contribute to the control of this disease. To explore potential genes related to the powdery mildew (Pm) resistance, in this study, we used a resistant genotype YD588 to investigate the potential resistance components and profiled its expression in response to powdery mildew infection. Genetic analysis showed that a single dominant gene, tentatively designated PmYD588, conferred the powdery mildew resistance in YD588. Using bulked segregant RNA-Seq (BSR-Seq) and SNP association analysis, two high-confidence candidate regions were confirmed in chromosome arm 2B, spanning 453,752,054-506,356,791 and 584,117,809-664,221,850, respectively. To confirm the candidate region, molecular markers were developed by the BSR-Seq data and mapped PmYD588 to a 4.2 cM interval by the markers YTU588-004 and YTU588-008. The physical position was subsequently locked into the interval of 647.1-656.0 Mb, that was different from those of Pm6, Pm33, Pm51, Pm52, Pm63, Pm64, PmQ, PmKN0816, MlZec1 and MlAB10 on the same chromosome arm in its position, suggesting it is most likely a new Pm gene. To explore the potential regulatory genes of the R gene, 2,973 differentially expressed genes (DEGs) between the parents and bulks were analyzed through GO, COG and KEGG pathway enrichment analysis. According to these information, we selected 23 potential regulated genes in the enriched pathway of plant-pathogen interaction and detected their temporal expression patterns using an additional set of wheat samples and time-course analysis post-inoculation with Bgt. As a result, six disease-related genes showed distinctive expression profile after Bgt invasion and can served as key candidates for the dissection of resistance mechanism and improvement of durable resistance to wheat powdery mildew.
Blumeria graminis
Candidate gene
Bulked segregant analysis
Common wheat
Cite
Citations (0)