Manipulation of Protein Translocation through Nanopores by Flow Field Control and Application to Nanopore Sensors
38
Citation
39
Reference
10
Related Paper
Citation Trend
Abstract:
The control of biomolecule translocation through nanopores is important in nanopore protein detection. Improvement in current nanopore molecule control is desired to enhance capture rates, extend translocation times, and ensure the effective detection of various proteins in the same solutions. We present a method that simultaneously resolves these issues through the use of a gate-modulated conical nanopore coupled with solutions of varying salt concentration. Simulation results show that the presence of an induced reverse electroosmotic flow (IREOF) results in inlet flows from the two ends of the nanopore centerline entering into the nanopore in opposite directions, which simultaneously elevates the capture rate and immobilizes the protein in the nanopore, thus enabling steady current blockage measurements for a range of proteins. In addition, it is shown that proteins with different size/charge ratios can be trapped by a gate modulation intensified flow field at a similar location in the nanopore in the same solution conditions.Keywords:
Nanopore
Biomolecule
Abstract Solid‐state ion nanochannels/nanopores, the biomimetic products of biological ion channels, are promising materials in real‐world applications due to their robust mechanical and controllable chemical properties. Functionalizations of solid‐state ion nanochannels/nanopores by biomolecules pave a wide way for the introduction of varied properties from biomolecules to solid‐state ion nanochannels/nanopores, making them smart in response to analytes or external stimuli and regulating the transport of ions/molecules. In this review, two features for nanochannels/nanopores functionalized by biomolecules are abstracted, i.e., specificity and signal amplification. Both of the two features are demonstrated from three kinds of nanochannels/nanopores: nucleic acid–functionalized nanochannels/nanopores, protein‐functionalized nanochannels/nanopores, and small biomolecule‐functionalized nanochannels/nanopores, respectively. Meanwhile, the fundamental mechanisms of these combinations between biomolecules and nanochannels/nanopores are explored, providing reasonable constructs for applications in sensing, transport, and energy conversion. And then, the techniques of functionalizations and the basic principle about biomolecules onto the solid‐state ion nanochannels/nanopores are summarized. Finally, some views about the future developments of the biomolecule‐functionalized nanochannels/nanopores are proposed.
Nanopore
Biomolecule
Cite
Citations (129)
Inside Back Cover In article number 2100542, Tsutsui, Baba, Kawai, and co-workers demonstrated the use of a 3D integrated nanopore system for in situ detections of single molecule proteins and DNA in a cell by ionic current. With the proven potential of nanopore sequencing, the present device technology promises amplification free genome analysis that may revolutionize biology and medicine through uncovering genetic variations at a single cell level.
Nanopore
Cite
Citations (1)
Rapid progresses have been achieved in one of the most promising third-generation sequencing methods: nanopore sequencing during recent years. Optical nanopore sequencing shows visible potential as the latest method. By optically encoding the ionic flux through protein nanopores, such as α-haemolysin and MspA, in a single droplet interface bilayer, the discrimination and detection of nucleic acid sequences can be parallelized. Nanopore blockades can discriminate between DNAs with sub-picoampere equivalent resolution, and specific miRNA sequences can be identified by differences in unzipping kinetics. If completely developed, this method will greatly increase the speed of sequencing after overcoming the sacrifices in device size and cost.
Nanopore
Cite
Citations (0)
We synthesized gating nanopores with embedded nanogap electrodes in a solid-state nanopore using an 11-step nanofabrication process. We were able to detect Au nanoparticles passing through a 30-nm-diameter gating nanopore via an electric current between nanoelectrodes. The electric current was proportional to the duration of translocation time. The gating nanopore is expected to be a next-generated nanosystem that can be applied to single-molecule sensors.
Nanopore
Cite
Citations (49)
Nanopore
Biomolecule
Cite
Citations (0)
Future of nanopore DNA sequencing: schematic illustration shows the future of nanopore DNA sequencing by using a customized biological nanopore with appropriate fabrication.
Nanopore
Cite
Citations (23)
Nanopore
Cite
Citations (0)
Nanopore
Sanger sequencing
Cite
Citations (0)
DNA and amino acids are important biomolecules in living organisms. Probing such biomolecules with structural characters can provide valuable information for life study. Here, gold plasmonic nanopores (GPNs) with high SERS activity (a local enhancement factor higher than 109) are synthesized at the tip of a glass nanopipette. An electric field drives individual molecules to translocate through the GPNs, which enables in situ collection of the surface-enhanced Raman scattering (SERS). Nonresonant biomolecules, including nucleobases, amino acids, and oligonucleotides (DNA), with single nucleobase differences can be distinguished. The intensity of SERS is tunable by modulating the affinity between DNA and the GPNs. The present study shows the feasibility of applying a plasmonic nanopore to DNA and protein detection, which may also provide an easy way for tracking single molecule translocation by developing a well-defined single plasmonic nanopore.
Nanopore
Biomolecule
Nucleobase
Cite
Citations (38)
Much tremendous break through have been obtained in recent years for nanopore sequencing to achieve the goal of $1000 genome. As a method of single molecule sequencing, nanopore sequencing can discriminate the individual molecules of the target DNA strand rapidly due to the current blockages by translocating the nucleotides through a nano-scale pore. Both the protein-pores and solid-state nanopore channels which called single nanopore sequencing have been studied widely for the application of nanopore sequencing technology. This review will give a detail representation to protein nanopore and solid-state nanopore sequencing. For protein nanopore sequencing technology, we will introduce different nanopore types, device assembly and some challenges still exist at present. We will focus on more research fields for solid-state nanopore sequencing in terms of materials, device assembly, fabricated methods, translocation process and some specific challenges. The review also covers some of the technical advances in the union nanopore sequencing, which include nanopore sequencing combine with exonuclease, hybridization, synthesis and design polymer.
Nanopore
Cite
Citations (39)