Observations onFasciola hepaticain donkeys from Morocco
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Abstract:
Regular monthly examination of a total of 422 donkey livers revealed Fasciola hepatica infection throughout most of the year, with an annual prevalence of 5.9% (range 0-20%). The monthly mean number of worms per animal varied from 0 to 6.4 (annual average 1.6). Of the infected animals 40% had less than ten worms, 28% between ten and 40, 24% between 41 and 70, and 8% over 71. The maximum number of flukes recovered from a single animal was 87. There were two peaks of infection, one in July (from the spring snail infection) and the other in December-February (from the snail infection of late summer-autumn).Keywords:
Hepatica
Fasciola
Fasciolosis
Abstract This study aimed to demonstrate the existence of a possible correlation between the level of liver fluke infection in cattle caused by Fasciola hepatica and the titers of anti-parasite antibodies in the blood. 113 blood samples were taken from non-dewormed pasture cattle in the communal slaughterhouse of the Jijel city in order to determine the serological titration of anti-fluke antibodies by the ELISA method. After slaughtering the animals studied, a count of parasites present in the inspected livers was carried out in order to assess the level of parasitic infection. The prevalence of bovine fasciolosis (due to Fasciola hepatica ) based on counting the number of flukes in the inspected livers was around 61.06%, while the seroprevalence obtained is 55.75%. The sensitivity and specificity of the ELISA test used in this study were, respectively, 91.3% and 100%. A very strong correlation (r = 0.93 to p < 0.0001) was statistically demonstrated between the antibody titers and the number of flukes in the inspected livers. According to the results of this study, there is a strong statistical correlation between serum antibody titers and the number of flukes present in the liver (the higher the number of parasites in the liver, the higher the level of anti-flukes antibodies in the blood).
Fasciolosis
Hepatica
Seroprevalence
Fasciola
Antibody titer
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Hepatica
Fasciolosis
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Background. Fasciolosis is one of the most common zoonoses in the world, caused by the trematode Fasciola hepatica L. This helminthiasis leads to significant economic damage in animal husbandry and can pose a direct threat to human health. Today, in the context of climate change, detailed knowledge of the biological characteristics of the trematode F. hepatica circulation will make it possible to predict outbreaks of fasciolosis on farms in detail and plan effective measures to combat this dangerous disease. The purpose of this work is to clarify some aspects of F. hepatica biology within Berdychiv district belonging to the forest-steppe zone of Zhytomyr region. Material and Methods. The work was carried out during 2020–2021. Collections of mollusks from water bodieson the territory of Berdichevsky district of Zhytomyr region served as the material for the research. Their identification was carried out by conchological features. Conventional methods were used for the parasitological study of mollusks. In total, more than 2000 mollusks Lymnaea (Galba) spp. were examined. Sampling of water and mollusks was carried out from four pasture reservoirs. Results. The main intermediate host of F. hepatica is the mollusk L. (G.) subangulata in the territory of Berdychiv region; L. (G.) truncatulaplays a secondary role in the distribution of this trematode. It was noted that the carriers of F. hepatica partenites are mainly mollusks with a shell height of 4.0 to 8.2 mm. New data on the dynamics of infection of L. (G.) subangulata with the trematode F. hepatica was obtained. The dynamics of infestation of these mollusks with F. hepatica cercariae shows a stable uniform character in the form of a two-peak curve, with peaks in May (2.51 ± 0.88 %) and August (9.19 ± 1.78 %). The overall prevalence of L. (G.) subangulata invasion by fasciola cercariae in the studied water bodies in 2020 was significantly lower than in 2021. Conclusions. In modern climatic conditions, in the territory of the region, permanent water bodies have become fasciolosis foci and common places for the settlement of L. (G.) subangulata, infected with F. hepatica cercariae. The most favorable environmental conditions for fasciola and its intermediate hosts develop in spring; accordingly; for the definitive hosts, this period is quite dangerous in terms of infection of animals.
Hepatica
Fasciolosis
Lymnaea
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Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
Fasciolosis is a parasitic disease of medical and economic importance. This retrospective study was conducted on 110 Fasciola flukes collected from livers of 14 infected Naimi sheep slaughtered at Riyadh abattoir in Saudi Arabia during winter season of 2016. Collected specimens were analyzed for their species identification on the basis of partial sequences of mitochondrial 28S rRNA gene. Results have shown the presence of both Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica) species. Where Fasciola hepatica was predominate (80%). Both intra-species and interspecies genetic distance was studied and results showed that the intraspecific variability among individuals of both species i.e., F. hepatica and F. gigantica, ranging between 0 and 1% while the interspecific diversity between F. hepatica and F. gigantica was only 1%. In conclusion, mitochondrial 28S rRNA gene is a proved as a good marker in identifying Fasciola of different species. Where, the F. hepatica and F. gigantica are present in sheep breed in Riyadh region, Saudi Arabia.
Hepatica
Fasciolosis
Fasciola gigantica
Fasciola
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Fasciolosis is a disease of sheep, cattle, goats and occasionally humans. It is caused by a trematode called Fasciola with the two most common species of Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica). The parasites encyst in the bile ducts and liver parenchyma of animals. Fasciolosis is common in marshy water bodies where favorable for its intermediate host. Snails of the genus Lymnae facilitate its survival and ubiquity worldwide. The disease causes serious economic losses annually, either directly or indirectly, by disrupting animal production. Therefore; this introductory review highlights on the occurrence, epidemiology, diagnosis, treatment, prevention and control of fasciolosis.
Fasciolosis
Fasciola gigantica
Hepatica
Fasciola
Parasitic Disease
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Fasciolosis, caused by the liver fluke (Fasciola hepatica) is an important issue for both human and animal health. The disease evokes economic losses which are a consequence of impaired animal productivity leading to higher costs of meat and milk production, as well as liver condemnation. The goals of this thesis were to: (1) elaborate a molecular method--PCR for the detection of F. hepatica DNA in intermediate and definite hosts; (2) estimate the usefulness of a recombinated cysteine proteinase produced in E. coli in the form of inclusive bodies in serological diagnosis of F. hepatica infection in definite hosts, using an enzyme-linked immunosorbent assay (ELISA); (3) conduct field research on the prevalence of infection among intermediate and definitive hosts (cattle) in chosen regions of Poland, utilizing the elaborated methods. Based on the results obtained in this study, it was established that it is possible to detect F. hepatica DNA in the feces of definite hosts with the elaborated PCR method. The amplification of a 124 base pair tandem repeat allows the detection of fluke larval stages in intermediate hosts within 12 hours of exposure and F. hepatica infection in definite hosts (by the 5th week in rats, 8th week in sheep and 10th week in cattle). Therefore, the PCR test is more sensitive than traditional microscopic methods. Furthermore, it was determined that, the recombinated cysteine proteinase in the form of inclusive bodies, after solubillization exhibits antigenic properties of the native protein and the ELISA method based on this antigen may be useful as a tool for diagnosing fasciolosis in sheep and cattle, in both serum and milk samples. The test achieves a greater sensitivity and specificity than an ELISA based on native excretory-secretory antigens. The results of field research indicate that Fasciola hepatica is a frequent parasite of cattle in central and eastern Poland. The mean prevalence was 34.86% (+/- 16.95) in all studied areas. The prevalence among intermediate hosts varied greatly (0-100%). The elaborated tests were proved to be valuable, mutually complementing diagnostic tools, applicable to different epidemiological situations.
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Fasciolosis
Parasitic Disease
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Hepatica
Fasciolosis
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Fasciola hepatica, a liver fluke of sheep and cattle, may accidentally infect humans. The main signs and symptoms of the fasciolosis are eosinophilia, abdominal pain and hepatomegaly and may also be attributed to Cystic Echinococcosis (CE) caused by Echinococcus granulosus, which is a prevalent infection in Turkey.Sera samples of 226 CE suspected cases were tested for anti-F. hepatica antibodies by an excretory secretory ELISA (ES-ELISA) and for anti-E. granulosus antibodies by ELISA and indirect hemagglutination (IHA) tests. Cases which were seropositive for fasciolosis were further evaluated radiologically and examined for F. hepatica eggs.Five (2.2%) and 96 (42.4%) of the 226 CE suspected cases were found seropositive for fasciolosis and CE, respectively. Although the radiological findings strongly suggested that there was fasciolosis in three cases, F. hepatica eggs were detected in two patients only.These data suggest that human fasciolosis is not as rare as previously reported in Turkey. F. hepatica infection should be suspected especially in the presence of eosinophilia, abdominal pain and liver lesions.
Fasciolosis
Hepatica
Fasciola
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Hepatica
Fasciolosis
Metabolic pathway
Fasciola
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Fasciola gigantica
Hepatica
Fasciolosis
Fasciola
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