[Permanent wound cover using cultured autologous epidermis transplants: a new treatment method for patients with severe burns].
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Epidermis (zoology)
Skin transplantation
Severe burn
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To investigate the effect of topical application of leptin in promoting burn wound healing in rats.Four parallel second-degree burn wounds induced on the back of 18 Wistar rats were divided into leptin treatment group (treated with topical application of 400 ng/ml leptin dissolved in PBS) and control group (treated with PBS). The time of wound healing was recorded, and the wound area that was not healed was measured at 3, 7, 11, 15, and 19 days after burns. The tissue at the peripheries of the wound was sampled at 7, 14 and 21 days after burns for pathological examination with HE staining and immunohistochemistry for proliferating cell nuclear antigen (PCNA) to evaluate the proliferation of keratinocytes.Compared with the control group, leptin-treated wounds showed a shorter time (by 2-3 days) of wound healing, and significant differences were found between the two groups in healing at 7, 11, 15, and 19 days after burns. HE staining and immunohistochemistry revealed a faster rate of epidermis growth and a greater thickness of the cuticular layer in leptin-treated wounds at 7, 14 and 21 days after burns. PCNA positivity in the keratinocytes was stronger in leptin-treated wounds than in the control wounds at 7 and 14 days, but no such distinct difference was noted at 21 days between the two groups.Topical application of leptin can promote re-epithelization in burn wounds to shorten the wound healing time of burns.
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Transplantation of autologous cultured epidermal sheets is a recent advance in the treatment of burn patients. A small skin biopsy is sufficient to produce large epidermal sheets within 3 weeks which provide transplants to cover extensive burn wounds. First clinical experience with this method is described.
Epidermis (zoology)
Skin transplantation
Severe burn
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The results of these studies are the basis for using a wound dressing based on bacterial cellulose in the comprehensive pathogenetic therapy of burn wounds. The process was studied on a model of III B degree planar burn wounds of rabbits. The experimental studies of the efficiency of the Derma RM wound covering have shown that its use in local treatment of uninfected deep burns prevents complications of the process of wound healing, and reduces the wounds healing time.
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Background: The problem of permanent skin coverage in severely burned patients is a question which, despite progress in the last few years has still not found a definite solution. The use of homograft is a way of avoiding graft rejection and reducing patient morbidity and a case in which it was used is hereby presented. Patient: A 14 year old boy presented with a 31% burn wound which was auto-grafted with meshed skin graft. The wound got infected and there was a total loss of graft. A skin graft from his twin was used to cover the wound. Results : Seven month post-surgery, his scar has remained stable. Conclusion: The use of homograft reduces patient morbidity and overall hospital stay. This method still has a place in extreme cases. Keywords: Burns, skin grafting, homo-grafting Nigerian Journal of Plastic Surgery Vol. 9, No 2, September 2013
Skin grafting
Severe burn
Skin transplantation
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An apparatus to produce shallow linear burns is described. The burns contained about the same amount of damaged tissue as a shallow cut. The initiation of healing in cuts and burns on guinea-pig ears was studied using an 3H-thymidine and autoradiographic technique to determine the number of cells in DNA synthesis in epidermis adjacent to the wound. Cuts caused epidermal cells to enter DNA synthesis rapidly; the maximum response was 1 day after injury. Thus response was delayed in burns and the maximum was not seen until at least 3 days after burning. Extracts of normal skin stimulated epidermal DNA synthesis, whereas extracts of burned skin did not. Possible mechanisms affecting the delay in the healing of burns are discussed.
Epidermis (zoology)
Thymidine
Skin transplantation
Pig skin
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One of the problems of burn treatment is a creation of conditions providing most valuable skin rehabilitation. An experimental model of burn wound healing in a 0.9% NaCl solution is proposed. Synthetic activity of rat epidermis cells in the process of burn wound healing under a scab and in liquid environment was studied by luminescent microscopy. The effect of a 0.9% NaCl solution involves an increase of the basal layer cell synthetic activity of regenerating epidermis, and keeping a high level of this activity of hair follicle epithelial cells for a long time. Tissue-preserving effect of the 0.9% NaCl solution on burns healing has been confirmed in these results.
Epidermis (zoology)
Severe burn
Artificial skin
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Objectives:To elucidate the role of stromal-derived factor-1(SDF-1)during wound healing and detect the distributions of CXCR4 in skin.Methods:ELLSA were conducted to measure the levels of SDF-1 in human burn blister fluids,which were collected 0-96 hours after burn.We also detected the expression of SDF-1 in rats' skin 1 day,3 days and 7 days after wound and the distributions of CXCR4 in skin by immunohistochemistry.Results:Results indicated SDF-1 in human burn blister fluids began increasing only a few hours after injury,reached the plateau level after 1 day,and then decreased gradually in 3 days postburn.CXCR4 is expressed at epidermal cells,and higher expressions were showed at the epidermal cells more near to basal layer.Conclusion:The increase of SDF-1 expression after skin wound may contribute to the wound healing.
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Basal (medicine)
Human skin
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Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.
Epidermis (zoology)
Severe burn
Animal model
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Eschar
Skin grafting
Immunosuppression
Wound Closure
Third-Degree Burn
Thermal burn
Skin transplantation
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Burns are injuries on the skin or other tissues. Burns are divided into superficial, partial, and full-thickness, characterized by the depth of the affected tissues. Histological analysis is critical to assess the burn wound healing process. Thus, a systematic evaluation system is imperative for burn research. In the present study, a total of thirty Sprague-Dawley rats were randomly divided into five groups. Deep partial-thickness burn wound was induced on the dorsal part of the rats. Six animals from each group were sacrificed on the 3rd, 7th, 11th, 14th and 21st day post-burn, respectively. Half of the wound tissue was immediately fixed in buffered neutral formalin for hematoxylin & eosin staining. The healing of the epidermis was evaluated with scores ranging from 0 to 7 based on the state of crust on wound surface, the degree of epithelialization as well as the formation of rete ridges. Meanwhile, healing of the dermis was also evaluated with scores ranging from 0 to 7 according to the proportion of adipose cells, inflammatory cells and fibroblasts, the state of collagen deposition as well as the formation of hair follicles. Furthermore, temporal changes of histological score of epidermis and dermis in the skin tissue with deep partial-thickness burn was evaluated. In conclusion, a new comprehensive system for assessing microscopic changes in the healing process of deep partial-thickness burn wound in hematoxylin & eosin staining slides was established, which simplified the scoring process and helped to obtain reproducible and accurate results in the burn study.
Thermal burn
Epidermis (zoology)
Histology
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