[Comparative phase-microscopical studies on protective epithelium of the Glisson's capsule and on aortic endothelium].
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Abstract:
Die Deckepithelien der Leberkapsel und das Aortenendothel bilden in den tiefen Schichten ein syncytiales Netzwerk. Oberflachlich besitzen diese Zellen homogene, hyaloplasmatische Deckplatten, die beim Aortenendothel in Ringerlosung stark quellen konnen.Keywords:
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Aim: To study oral hyperplastic epithelium, dysplastic epithelium and squamous cell carcinoma to determine (1) the prevalence of p53 protein immunoreactivity, (2) number of p53 positive cells, and (3) the area of localization of p53 protein immunoreactivity. Methods: Two contiguous sections from 30 tissue specimens (10 each from oral hyperplastic epithelium, dysplastic epithelium and squamous cell carcinoma) were subjected to hematoxylin and eosin (H/E) staining for histopathological diagnosis and immunohistochemical (IHC) staining for demonstration of p53. p53 positivity was looked for in each IHC stained slide and the number of positive cells amongst 1,000 epithelial cells were recorded. The localization of these p53 positive cells within the strata (i.e. basal/suprabasal, spinous and superficial layers) of epithelium between 3 groups, and also within each group according to histological grades was recorded. Results: Higher p53 positive cell counts were demonstrated in oral squamous cell carcinoma compared to hyperplastic and dysplastic tissues. The expression of p53 in epithelial hyperkeratosis was mainly localized to basal epithelial cells whereas in epithelial dysplasia, it was predominantly localized to spinous epithelial cells. Conclusions: Qualitatively p53 is not a specific marker for malignancy of oral epithelium. However the quantitative analysis of p53 positive cells and their localization in oral epithelium is of importance as a marker for oral squamous cell carcinoma.
Epithelial dysplasia
Basal (medicine)
Oral mucosa
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The expression of epithelial markers (cytokeratins, Filaggrin. BerEp4 and EMA), collagen IV and Ki67 was studied immunohistochemically in cholesteatoma and compared with that in epidermis of meatal skin, squamous epithelium of eardrum and simple epithelium of middle ear mucosa. MNF116 (cytokeratin 10, 17, 18) stained the full layer of normal epithelium and all cholesteatoma specimens. CK10 and Filaggrin were expressed in the upper layer of epidermis but more diffusely in cholesteatoma. BerEp4 was found in the basal layer of normal epithelium but was detected in most epithelial cells in cholesteatoma matrix. Variability was observed in EMA and CK14 immunostaining. Collagen IV was localized in the basement membrane of normal epithelium with a continuously staining pattern, an observation also made in the cholesteatomas studied. However, in one of these small areas the basement membrane was not stained with collagen IV. Ki67 was expressed in nuclei of the cells in the basal layer of normal epithelium but extended to epithelial cells in the upper layers of cholesteatoma matrix. The results of the present study indicate that the expression pattern of epithelial markers in cholesteatoma corresponds to that in normal epidermis. The increasing expression of BerEp4 and Ki67 confirms the hyperproliferative nature of cholesteatoma. Whether or not the lack of expression of collagen IV in one of the cholesteatomas reflects a true degradation of the basement membrane needs further investigation in extended materials.
Epidermis (zoology)
Immunostaining
Basal (medicine)
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Oral mucosa
Basal (medicine)
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Abstract: The pathological lining epithelium of destructive periodontitis was studied by analysis of the expression of intermediate filament proteins in biopsies of untreated advanced periodontitis. The cytokeratin (CK) pair 8/18 characteristic of simple epithelia was expressed consistently in a distribution pattern confined to the reactive pocket epithelium. The pattern of CK8/18 expression was complex with two broad presentations evident. In two‐thirds of the advanced disease biopsies, the entire pathological lining epithelium was strongly reactive for both CK8 and CK18. In the remainder, the more superficial lining epithelium was mixed with foci of reactive and unreactive cells, with the deeper epithelium uniformly reactive. Only occasional highly localised reactivity for the simple keratins (CK8/18) was found in the lining epithelia of biopsies from minimally inflamed periodontal tissues. The pathological lining epithelium of advanced periodontitis was further characterised by the co‐expression in basal layers of CK14, and of CK13 but not CK4, which are characteristic of suprabasal layers of stratified squamous epithelia. Cytokeratin 17, a marker of high turnover and migrating epithelial cells was extremely variable with no clear association between expression pattern and location of the epithelium or disease status. There was no reactivity for CK10/11 typical of cornifying cells nor of vimentin, the characteristic intermediate filament of mesenchymal cells. The intermediate filament protein profile of the reactive lining epithelium was indistinguishable from the reactive epithelium present in three of five biopsies of periapical granulomas containing hyperplastic epithelium from activation of the developmental remnants of Hertwig’s sheath, known as the cell rests of Malassez. The data reported are compatible with a contribution by remnants of developmental epithelium, including the reduced enamel epithelium and the cell rests of Malassez, to the reactive lining epithelium of the subgingival pocket in the pathogenesis of chronic periodontitis.
Enamel organ
Stratified squamous epithelium
Keratin 14
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Abstract Endoscopic ultrasound‐guided pancreatic fine‐needle aspiration biopsy very frequently produces gastrointestinal epithelial contamination (GIC). We studied the cytomorphology and B72.3 immunoreactivity of lesional epithelium of benign and malignant ductal lesions of the pancreas and compared the findings to our previously established template of GIC. Air‐dried smears, fixed smears, and ThinPrep® (TP) specimens were obtained using a cytobrush, directly from benign and malignant ductal lesions of 18 Whipple specimens, to ensure purity of the epithelium studied. Smear background, cell architecture, and cellular features were analyzed. Immunocytochemical staining with B72.3 was performed in 14 cases. Epithelium of ductal carcinoma was distinguished from benign ductal epithelium in chronic pancreatitis and GIC primarily by crowded architecture and atypical cellular features, including high nuclear‐to‐cytoplasmic ratio, irregular nuclei, nucleoli, and vacuolated cytoplasm. Benign ductal and GIC epithelium were only distinguished by architecture (goblet cells and brush borders), but not consistently, especially gastric epithelium that lacked these features. B72.3 shows promise in the differentiation between GIC and benign and malignant ductal epithelium, with no staining supporting benign ductal cells, fine punctate perinuclear staining correlating with GIC, and strong cytoplasmic staining supporting malignancy. Diagn. Cytopathol. 2007;35:300–305. © 2007 Wiley‐Liss, Inc.
Gastrointestinal epithelium
Ductal cells
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An 18‐year‐old woman with abdominal pain was diagnosed as having splenic cysts by computed tomography scan. She had high serum levels of CA19‐9 (2886.8 U/mL; normal value, <35 U/mL), CA125 (131.1 U/mL; normal value, <35 U/mL) and soluble IL‐2 receptor (1490 U/mL; normal range, 220–530 U/mL). The resected spleen weighed 1050 g, was 14 × 28 cm, and had more than 10 macroscopic cysts up to 10.3 × 9.5 cm. There were numerous microscopic cysts in the spleen and several on the splenic capsule. The levels of CA19‐9 and CA125 in the cyst fluid were 2 165 550 U/mL and 160 400 U/mL, respectively. After the surgery, the serum levels of the tumor markers decreased gradually. The inside of the largest cyst was mainly covered by granulation tissue with a focal lining of epithelial cells, and the other macroscopic cysts had stratified squamous epithelium. The microscopic splenic cysts and cysts on the splenic capsule were lined by either attenuated single‐layered or multilayered epithelial cells. The lining epithelial cells of these cysts were positive for epithelial membrane antigen and cytokeratins. CA19‐9 and CA125 were detected in the lining cells of the splenic cysts. In the present case, it is suspected that the splenic cysts were derived from the capsular lining cells that showed migration from the capsule or formed microcysts on the splenic capsule, as in the case of ovarian inclusion cysts.
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Using the double label indirect immunofluorescence technique we have studied vimentin-positive cells present in normal ecto- and endocervical epithelium, subcolumnar reserve cell hyperplasia, and squamous metaplastic and dysplastic epithelium of the uterine cervix. Monoclonal antibodies to Ia-and T6-antigens were applied in the examination of the expression of these membrane markers by such cells. Our studies reveal the presence of a relatively large number of vimentin-positive and T6-positive (Langerhans) cells in normal ectocervical stratified squamous epithelium, a small number in endocervical columnar epithelium, and a larger number in subcolumnar reserve cell hyperplasia and in immature squamous metaplasia. In this respect, mature squamous metaplastic epithelium shows a great resemblance to normal ectocervical stratified squamous epithelium. In contrast with previous reports in the literature we could only demonstrate small numbers of Langerhans cells in cases of dysplasia. The clinicopathological significance of these findings is discussed.
Stratified squamous epithelium
Squamous metaplasia
Metaplasia
Endocervix
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ABSTRACT The purpose of this paper is to describe a simple technique for the shortterm cultivation of adult mammalian skin epithelium in vitro. The conditions of cultivation are such as allow the rapid proliferation and migratory spread of skin epithelium while maintaining the characteristic functional activity and histological appearance of its cells. The technique was devised to investigate the effect of iso-antibodies on cells. It requires little, if any, modification for use as an adjunct to routine biochemistry (e.g. in studying the influence of specific metabolic inhibitors on cell movement and division: cf. Medawar, 1947), or for submitting cells to the direct action of hormones. It has the disadvantage that the cultivated cells do not grow away from the explanted tissue into the culture medium, so that the direct microscopic study of living cells (an amenity to which, in conventional tissue culture, most others have been sacrificed) must be forgone. The tissue can, however, be studied in the transformed state in which it is most familiar to histologists: in stained sections.
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ABSTRACT In number 17 of the ‘Centralblatt f. med. Wiss.,’ 1879, I have described giant nuclei of the huge epithelial cells lining the large saccular glands of the skin—tail—of newt (Triton cristatus). I have shown that these nuclei, when examined fresh in aqueous humor of frog, show an exceedingly distinct network of more or less uniform fibrils and trabeculæ ; owing to the large size of the nuclei their network can be seen in all its details even under a low power. Many of these giant nuclei show in the network of the highly refractive fibres and trabeculæ cylindrical or irregular accumulations corresponding to nucleoli, others are free of them. I have also shown that both the nucleoli, when they exist, and the fibrils and trabeculæ, possess vacuoles ; and further, that on the warm stage the intranuclear network shows contraction, whereby the outline of the nucleus changes in a similar manner as in cells while undergoing amoeboid movement.
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