Myeloid differentiation antigens identify leukemic cell subpopulations with different cell cycle characteristics.
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CD15
Cluster of differentiation
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To explore megakaryocytic (MK) antigen expression in previously untreated adult acute myeloid leukemia (AML) and its relation to the clinical and biological characteristics.Two hundred and eleven cases of AML were detected with flow cytometric immunofluorescence assay.Twenty-seven cases (12.8%) were MK antigen positive with the higher positive rates in hybrid acute leukemia (45.5%) and acute monoblastic leukemia (24.1%). MK antigen expression was significantly correlated with CD34 antigen expression, high white cell count, high P-glycoprotein positivity and had no correlation with chromosome aberration. 33.3% of MK positive AML patients achieved complete remission which was significantly lower than that (71.9%) in MK negative cases.MK antigen positive AML might derived from malignant transformation of hemotopoietic stem cell at earlier stage and the detection of MK expression was of values in predicting treatment effect and prognosis for adult AML.
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Leukemia cells express a variety of cell surface antigens that have been useful in the delineation of subclasses of both lymphocytic and myeloid leukemia. In this report we describe the phenotype of cells from 50 patients with leukemia based on the results of testing for binding to four monoclonal antibodies reactive with myeloid cell-associated antigens and, in some cases, three monoclonal antibodies reactive with lymphocyte-associated antigens. We related the patterns of reactivity of these leukemia cells with the diagnosis based on morphology and histochemistry. Three of the myeloid cell-associated antigens (PMN 6, PMN 29, and AML-2-23) were expressed only on cells from patients with AML. The fourth, PM-81, was expressed on cells from 29/31 patients with AML and on 3/5 with ALL. The reactivity of the ALL cells with the J5 and B1 antibodies confirmed the diagnosis of ALL. A proposed schema for the use of these monoclonal antibodies in both the diagnosis and subclassification of leukemia is described. Finally, the significance of the heterogeneity in antigen density on leukemia cells is considered.
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Objective:To study the immunophenotype of acute leukemia and its clinical significance.Methods:A panel of lineageassociated monoclonal antibodies,CD 45 and direct immunofluorescence technique were used to analyse the immunophenotype in 34 cases of acute leukemia.Results:There were 8 B ALLs and 3 T Alls in 11 cases of ALL,in which of them 4 cases were expressed with myeloid antigens(36.4%),and 10 cases were expressed with CD 34 (90.1%).Meanwhile there were 7 cases with lymphoid antigens expression(29.17%),16 cases with CD 34 and DR expression (70.8%) in 24 cases of AML,and no CD 34 and DR antigen expression were found in 5 M 3 patients.Furthermore,CR rate in myeloid antigen positive ALL is slight lower than in that myeloid antigen negative ALL(1/3 vs 5/6),but there was no significant difference between them( P 0.05).CR rate in lymphoid antigen positive AML is markly lower than in that lymphoid antigen negative AML(0/5 vs 10/10, P 0.01).Conclusion:It was the best method by flow cytometry on CD 45 versus sidescatter gating for immunophenotype analysis of luekemia,and aberrant antigen expression in leukemia was recognized to be a fact of poorer prognosis.
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Objective To assess the clinical features,immunophenotypic,and cytogenetic characteristics of acute megakaryocytic leukemia(AMKL).Methods Leukemic cells from 20 patients were labeled with a panel of monoclonal antibodies by using indirect immunofluorescence or flow-cytometry as so to detect the expression rate of each monoclonal body.Results Twenty patients were positive in CD_(41a) and CD_(41b) while no expressions were detected in the relative monoclonal antibodies of the lymphocytic lineage.However,positive expressions were demonstrated in myeloid ones in 7 cases.No-stochastic aberrations of chromosomes were discovered in 10 cases.Conclusion Monoclonal antibodies by using immunofluorescence were a shortcut and expedient method in diagnosing AMKL.AMKL is a bad prognosis disease.
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A flow cytometric technique was used to detect granulocyte antibodies, with attention to the distinction between antibodies directed against surface and intracellularly expressed antigens. Ten serum samples with positive results and 10 with negative results detected by the granulocyte immunofluorescence test (GIFT ), together with 10 positive serum samples detected by an indirect immunofluorescence test (IIF) were analyzed against leukocytes from healthy blood donors tested by flow cytometry (FC). Unpermeabilized and permeabilized cells were used to allow identification of surface and intracellular binding, respectively. The results of testing by GIFT corresponded with those by FC, with the exception of the results for four sera: one serum sample was FC negative, GIFT positive, and three samples were FC positive, GIFT negative. The IIF-positive sera were all FC positive, analyzed against permeabilized granulocytes, and one serum was also positive against non-permeabilized granulocytes. Analysis by FC is a readily performed technique, which can be used for the routine detection of antibodies against leukocyte antigens. Screening for granulocyte-specific antibodies can be carried out with pools of granulocytes from three donors. Analysis by FC allows detection of both HLA antibodies and granulocyte-specific antibodies and by using both unpermeabilized and permeabilized cells, antibodies against surface and intracellular antigens, respectively, can be identified.
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To prepare and identify monoclonal antibody (mAb) against human vascular endothelial cadherin 5 (VECDH5).BALB/c mice were immunized with recombinant VECDH5 protein for preparing mAb using hybridoma technique. The positive clones were confirmed and selected by indirect ELISA for titer determination. Western blotting, flow cytometry, immunofluorescence staining, immunohistochemical staining were performed to identify the specificity and epitope.One hybridoma cell strain secreting specific mAb against VECDH5 was obtained (2C11). ELISA showed that the titer of the ascites was 1:10 000. Western blotting, flow cytometry, immunofluorescence, immunohistochemistry demonstrated that the mAb could specifically recognize and bind VECDH5. Epitope identification showed that the amino acid sequence recognized by 2C11 was LDREVVPWYNLTVEA.We have prepared mAb against human VECDH5, which has good binding ability and specificity.
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