Capability of Mixed High Efficiency Bacterium Strains in Degrading Alkanes and Succinonitrile
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This paper presents an investigation of the bacterium strains of C-14-1,C-14-2 and J-13-1 with high degrading capacity for alkanes and succinonitrile respectively.Tests on the mixed bacteria in the mixed system containing multifactor substrate show that since there is no antagonism among strains C-14-1,C-14-2 and J-13-1,they can be mixed and applied together.Because of the effect of catabolite repression and synergistic mechanism between products,the degradation of succinonitrile is promoted while the degradation of alkanes is inhibited in the mixed system of culture medium.Owing to some inhibitting factors,the degradation capacity is depressed in the acrylic fiber wastewater.And the alkanes degrading bacterium strains are not efficient in degrading because of the alkane concentration in wastewater is low.On the other hand,with the succinonitrile concentration in wastewater becoming higher,the degradation result is becoming more obvious,and the degrading rates reach 100% after 9 h.The acrylic fiber wastewater is treated with the mixed bacteria for 9 h,the removal efficiency of CODCr is only 29%,which indicates that wastewater is so complicated that it is impossible to reach a good result in integral treatment just with several high active bacterium strains.It is necessary to construct high active population including many kinds of bacteria among which synergistic mechanism exists to reach a desirable effect in practical treatment.Keywords:
Succinonitrile
Degradation
Industrial wastewater treatment
Alkane
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Isolation
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In this study, the chlorpyrifos degradation differences of two degrading bacteria from two sources are measured and analyzed, one is X1, cultivated and isolated from the sludge of a city sewage treatment plant; the other comes from the long-term chlorpyrifos contaminated soil, named D. The results show that: the two degrading bacteria do not inhibit each other, degradation capacity of strain D is slightly stronger that the X1; the optimum temperature for degradation of the two strains are all 30°C, the requirements of the pH value is different, the X1 is in acidic and alkaline conditions while the strain D is in the neutral condition; the best addition of agent of X1 is 2mL and the D is 3mL; external carbon source on degradation of the two strains have a certain inhibitory effect, but nitrogen can promote; all the 10 tested metal ions inhibit the degradation ability of the two strains.
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A benzene-degrading bacterium(strain DQ0306) was isolated from oil-contaminated soil around one oil well in Daqing,China after a series of experiments.DQ0306 could take benzene as sole carbon and energy resources and degrade benzene efficiently with degrading rate 35.7%.The bacterial strain was initially identified as Flavobacterium based on its morphology,physiological,biochemical properties.The impact of bacteria itself and the environmental conditions on benzene degradation was also studied.The growth curve of DQ0306 and biochemical degradation curve of benzene by the bacterial strain were similar;they all entered exponential phase about 20 hours later and the two stationary phases appeared after over 44 hours.The optimal conditions for the degradation of benzene were 30 ℃,pH 6.5~6.7,inoculation 10%,shaking rotate 180~220 r·min-1,reaction time 32 h respectively.DQ0306 may provide a possible channel for improving the degradation efficiency of benzene hydrocarbons which are usually degraded rather difficultly in petroleum components.
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Screening seven strains of efficient phenol degradation bacterium in activated sludge from Harbin Taiping sewage treatment plant. These strains can use phenol as the only available carbon sources and energy. Based on inspects the growth and degradation of phenol in different temperature, pH value, and different phenol concentration. We can determine that the optimal temperature of seven bacterias is 10°C, the optimal pH value is 7.5, the lagest concentration of degradation of phenol is 3000 mg/L. From the study of degradation performance by these seven bacteriums shows that it has strong ability of phenol degradation. In the condition of 10°C, pH 7.5, fluid for 50 mL, vaccination is 15%, vibration velocity wave 160 r/min, degradation rate of 500 mg/L phenol can reach more than 90% after 48 h in the training. Glucose to the growth of bacteria and phenol degradation ability all have certain effects, when glucose concentration is 500 mg/L, the degradation rate of phenol is still more than 80%. The study on treatment contain other carbon sources of hydroxyl benzene wastewater has certain significance. Through the analysis DGGE strip map shows that the brightness can explain these bacteria are advantage bacterium in each system, and strong activityin sewage, its dominant position can be exist steadily. Rich strips are 2, 4, 24, 28, and it is showing its diversity in sewage system.
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Two Pseudomonas aeruginosa strains, S and Y, were used here to study the effects of n-alkanes substrate and environmental conditions, including temperature, pH and salinity on microbial hydrocarbon access modes and degrading ability. The results showed that both microbial hydrocarbon access modes, that is, direct contact mode and biosurfactant-medium mode, existed in the petroleum hydrocarbon degradation process by the same strain and were affected by the substrate and environmental factors. It was found that the uptake of long-chain n-alkanes was improved by the two modes, but high salinity was disadvantageous to the alkane uptake process in either of the modes. High temperature, alkaline solution and long degradation time were advantageous to utilization of n-hexadecane substrate by biosurfactant-medium mode, while low temperature and moderate pH were more suitable for hydrocarbon biodegradation by direct contact mode.
Alkane
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