The RAPD Study on the Spirulina platensis in Different Habitats
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Abstract:
The samples of Spirulina (Arthrospira) platensis from different habitats were analyzed by the RAPD. The result shows that there are obvious differences in the molecular level between the Spirulina (Ar- throspira) platensis from alkaline lakes in Erdos Plateau and the one from Chad Lake, and the homology of DNA in the amplified polymorphism of the Spirulina (Arthrospira) platensis only reaches 48.23%. The dif- ferent habitats and geographical isolation are the primary reasons for the above-mentioned result.Keywords:
Spirulina (dietary supplement)
Arthrospira
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In this paper,the hoxY gene of hydrogenase in Arthrospira were cloned and sequenced,which Arthrospira platensis from Alkaline Lakes in the Ordos Plateau,A. platensis from Lake Chad in Africa and A. maxima from Lake Texcoco in Mexico. And their homology and genetic relationship were also analysed,in the hope of providing elementary data and theoretical bases for the research of diversity of Arthrospira from Alkaline Lakes in the Ordos Plateau. The results showed that the partial sequence of hoxY gene in Arthrospira contained 479 bp. There was a high degree of sequence homology between A. platensis from Ordos and A. platensis from Lake Chad,A. maxima which were 99. 5%and 99. 9 % respectively. The genetic relationship between A. platensis and A. maxima were closer than that from A. platensis from Ordos. Conclusion: The gene hox Y had a high conservation,in which sequence of the different samples among the same genera had low variation. However,gene sequence of the same species of Arthrospira which grew in different environments has variation.
Arthrospira
Homology
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