The Changes in the Expression of CD44 of Rat with Hemisection of Spinal Cord Injury
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Objective To investigate the expression changes of CD44 in spinal cord in 3d,7d and 14d following hemisection of spinal cord injury(hSCI).Furthermore,to explore the relationships of CD44 and spinal cord injury.Besides,to offer the morphological datum for the research concerning the spinal cord repair.Methods 20 adult healthy Sprague-Dawley rats were randomly divided into sham-operated control group and day3,day7,day14 spinal cord injury groups.The spinal cords were hemisected between T9 and T10.The spinal cord of every rat was taken out from the rostral and caudal segments of lesioned areas.Then the tissue blocks were made into 25μm frozen sections and the immunohistochemistry ABC method was performed on these sections.We respectively observe and count the number of CD44 positive cells in spinal cord for all groups,and the average OD(optical density) value of immunoreactant were detected with computer image analysis technique.Results CD44 positive products were mainly distributed in extacellular matrix and neurons and neuroglial cells in spinal cord for the control group.The number of CD44 positive cells and its OD value of rostral or caudal part of injury site for the three spinal cord injury groups were all increased(P0.05).Conclusion The increased-expression CD44 may mutually play inhibitory effect on axon regeneration following SCI.Topics:
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Objective Changes of Aggrecan of expression in rats spinal cord following hemisection of spinal cord injury have been studied to provide the experimental evidence for the mechanisms of regeneration and repair after spinal cord injury.Methods 20 adult healthy Sprague-Dawley rats were divided into sham-operated control group and 3days,7days,14days spinal cord injury groups at random.The spinal cords were hemisected between T9 and T10.The T9～10 spinal cord was isolated and sectioned.Aggrecan was observed by immunohistochemical staining.And the immuno-positive gray scale of immunoreactant was detected with computer image analysis technique.Results Aggrecan positive products were mainly distributed in neurons and neuroglial cells in spinal cord and ECM for the control group.The immuno-positive expression for Aggrecan in three spinal cord injury groups was all increased as compared with cortrol group.Conclusion The expression of Aggrecan increases after HSCI,it may mutually play inhibitory effect on axon regeneration.
Aggrecan
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Lumbar Spinal Cord
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Nestin
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Objective: To study recovering of adult rat spinal cord hemisection by bridging lntercostal nerve to spinal cord nerve root and Glial cell Line derived neurotrophic factor(GDNF). Methods: Adult rats were divided into 3 groups: Group A received lumbar spinal hemisection only, Group B received lumbar spinal hemisection and bridging intercostal nerve to spinal cord nerve root, Group C received lumbar spinal hemisection and bridging intercostals nerve to spinal cord nerve root and GDNF. Combined behavioural Score(CBS) was recorded posteratively, somatosensory evoked potentials(SEP) and motor evoked potentials(MEP) were examined. Results: The CBS score sequence was (ABC) in 3 groups and peak latencies of early waves in SEP and MEP Sequence were ABC, showing statistically significant difference ( P0.05) . Conclusions: Bridging intercostals nerve to spinal cord nerve root and GDNF can improve the recovering of injured spinal cords of adult rats.
Lumbar Spinal Cord
Intercostal nerves
Spinal nerve
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Nerve fiber
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Objective:To set up a practical and reliable animal model of spinal cord injury.Methods:Spinal cord was hemi-sected C 5～7 and T 9～11 level.At different times after injury,the behavior and the histological change of lesioned cord was observed and evaluated.Results:Its showedthat the hSCI's lateral cord and substantia grisea was degenerated to necrosis under microscopy.It had significance the CBS assessment of study group than that of sham-operated group( P 0.05).The rat's spinal cord ventral horn's motor neuron had great changes of the motor and feel function.Conclusion:Hemi-sected of C 5～7 spinal cord has the advanges of low mortality and it is safe.
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Immunofluorescence
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Objective To study the characteristics of neural cells super microstructure after acute spinal cord in jury.Methods Following a controlled moderate degree compression injury to the lower thoracic spinal cord (T8.9)of rats and were sacrificed at 8 hours after injury. The segment of every spinal cord were cut for morphological studies.Results The cells in the compression region were fund different morphological characteristics mainly necrotic apoptosis and necrosis.Conclusion Our data suggested that morphological changes of neural cells after spinal cord injury is diversity.
Spinal cord compression
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Objective The present study was aimed at investigating the possibility of remaining spinal fibers and the relationship between such remaining fibers and the functional recovery of locomotion in the hindlimbs following spinal cord transection in adult rats. Methods The lower thoracic spinal cords of 12 adult rats were transected with a sharp blade along the inner wall of the vertebral canal. The functional recovery was evaluated and recorded regularly after surgery in each of the animals. Animals were slaugtered after a survival period of 8 weeks. The damaged segments of the spinal cords were sectioned sagittally and prepared for anti-neurofilement immunostaining as well as image analysis. Results 42% animals showed different degrees of functional recovery, which correlated well with the amount of remaining spinal fibers passing through the lesion site in the ventral or ventrolateral part of the cord. Conclusion Conventional spinal cord transection with sharp blade often leads to the remaining of the spinal fibers in the ventral or ventrolateral part of the cord and sequential functional recovery of the spinal cord. In view of this, the completion of spinal cord transection must be assured before any function recovery can be assessed.;
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