Inhibitory Effects of Composite PSP on HeLa Cells of Human Cervical Cancer
1
Citation
0
Reference
20
Related Paper
Citation Trend
Abstract:
[Objective] The aim was to discuss the effect of composite PSP on the proliferation of HeLa cells of human cervical cancer.[Method] PSP and GBE were compounded according to different doses and 3 proportions of 1∶1,1∶2 and 2∶1,and the inhibitory effect of the composite PSP on HeLa cells of human cervical cancer was studied by MTT method.[Result] The inhibitory effects of using PSP and GBE compoundly according to 3 proportions on HeLa cells of human cervical cancer were all obviously better than that of using single component at corresponding doses and the tumor inhibition rate of group at the same proportion showed positive correlation with dose.The combined use of PSP and GBE could produce synergy in inhibiting HeLa cells of human cervical cancer.Among them,the effect of PSP and GBE with the proportion at 2∶1 was most prominent and the tumor inhibition rates at high,middle and low doses were 67.92%,58.93% and 34.86%(P0.01),resp.[Conclusion] The study laid a foundation for curing human cervical cancer.Keywords:
HeLa
MTT assay
Cite
Prostate cancer, the most frequently diagnosed cancer in men, primarily affects males aged 55 and older and is more common in African Americans than Caucasians. Once the cancer has metastasized, current treatments are generally ineffective. We have identified a novel anti-neoplastic agent, a specifically designed nutrient mixture (NM), containing ascorbic acid, lysine, proline and green tea extract that demonstrates a broad spectrum of anti-tumor activity against a number of human cancer cell lines. In a previous study NM significantly inhibited prostate tumor in nude mice. In this study, we tested whether the formulation exerts its anti-tumor effects through induction of apoptosis on prostate cancer cell line DU-145. The effect of the nutrient mixture (NM) on cell growth inhibition in DU-145 cells was examined by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes and caspase activation associated with apoptosis induction was checked by H&E staining and Live Green Caspase assay, respectively. The NM was found to be slightly toxic to DU-145 cells at 100 μg/ml, but significantly toxic at 500 μg/ml and 1000 μg/ml. Percentage of cells undergoing apoptosis also increased from 6% at 100 μg/ml to 49% at 500 μg/ml and 83% at 1000 μg/ml, with greater number of cells showing morphological changes such as condensed nuclei and an acidophilic cytoplasm at higher concentrations. For the purpose of comparison, NM was also tested on a normal human dermal fibroblast (NHDF) cell line which exhibited far less apoptosis induction as compared to DU-145 cells. The percentage of cells undergoing apoptosis in case of NHDF cells was 7% at 100 μg/ml, 25.6% at 500 μg/ml and 76.5% at 1000 μg/ml. Our results demonstrate that the NM is effective in inhibiting cancer cell viability and inducing apoptosis in prostate cancer DU-145 cells and can thus be used as an effective treatment for prostate cancer.
Cite
Citations (9)
Objective: Danggui, the root of Angelica Sinensis, has traditionally been used for the treatment of women’s reproductive disorders in China for thousands of years. This study was to determine whether Danggui have potential anti-cancer effect on women’s cancer and its potential mechanism. Methods: Danggui was extracted by ethanol. The Cell Titer 96® Aqueous Non-Radioactive Cell Proliferation Assay was used to compare the effects of Danggui on human breast (MCF-7 and 7368) and cervical (CaSki and SiHa) cancer cells with its effects on normal fibroblasts (HTB-125). A revised Ames test was used to test for antimutagenicity. The standard strains of Salmonella typhimarium (TA) 100 and 102 were used in the test. Methyl methane sulfonate (MMS) and UV light were used as positive mutagen controls and ethanol and double distilled water (DDW) as controls. The SAS statistical software was used to analyze the data. Results: Danggui was found to be much more toxic to all cancer cell lines tested than to normal fibroblasts. There was a significant negative dose-effect relationship between Danggui and cancer cell viability. Average viability of MCF-7 was 69.5%, 18.4%, 5.7%, 5.7%, and 5.0% of control for Danggui doses 0.07, 0.14, 0.21, 0.32, and 0.64 ug/ul, respectively, with a Ptrend < 0.0001. Half maximal inhibitory dose (ID50) of Danggui for cancer cell lines MCF-7, CaSki, SiHa and CRL-7368 was 0.10, 0.09, 0.10 and 0.07 ug/ul, respectively. For the normal fibroblasts, ID50 was 0.58 ug/ul. At a dose of 0.32 ug/ul, Danggui killed over 90% of the cells in each cancer cell line, but at the same dose, only 12.3 % of the normal HTB-125 cells were killed. Revertants per plate of TA 100 decreased with the introduction of increasing doses of Danggui extracts with a Ptrend < 0.0001 when UV light was used as a mutagen. There was no difference in revertants per plate between ethanol and DDW control groups. Conclusions: Danggui could be used as a safe and effective adjuvant therapy to prevent and treat breast and cervical cancers. Anti-cancer effects may be due to its anti-mutagenicity. Danggui should be investigated as a potential adjuvant anti-cancer therapy for women’s cancer treatment and prevention of recurrence. Key words: Angelica Sinensis, Danggui, cancer, women’s reproductive disorders
Angelica sinensis
Viability assay
Cancer cell lines
Cite
Citations (3)
ObjectiveTo study the cytotoxic effects of ShuGanShuRu Granules(SGSR)on MCF-7 human breast cancer cells in vitro.MethodsBy using modified MTT assay,the influences of SGSR upon the OD values of MTT were studied and the cytotoxicity of SGSR on MCF-7 human breast cancer cell were monitored.ResultsThe OD values of the different concentrations of SGSR were all lower than that of controlled group,which indicated that all concentrations of SGSR have cytotoxic effects on MCF-7 cells,especially the killing rate of groups of 4,l,0.25g/ ml reached 99.9%,99% and 95.55% respectively.There were statistically striking differences(P0.01) and showed good dosage-effect relation.The killer rate is considerably higher than that of β-Carotine(β-CA) and β-CA added SGSR groups.ConclusionThis study reveals that SGSR has obvious cytotoxic effects on MCF-7 cells.Its mechanism might be associated with the anti-mutation effects as well as the direct killing activities,thus making DNA chains broken resulting in the death of tumor cells.
MCF-7
MTT assay
Cite
Citations (0)
Objective To explore the effects of melatonin combined cisplatin on the proliferation of lung cancer cells A549.Methods Lung cancer cells A549 were co-cultured with different concentrations of melatonin(100,300,500,700,1 000 μmol/L) and cisplatin(2.5,5,10 μg/mL)for 48h,respectively.The inhibitory rate of A549 cells were detected by MTT assay.Results When A549 cells were co-cultured with different concentrations of cisplatin(2.5,5,10 μg/mL) and melatonin(500 μmol/L),the inhibitory rates were high to(71.25±5.01)%,(86.37±7.10)%,(97.63±8.65)%,respectively.They were significantly higher than those inhitory rates of single cisplatin administration at the same dose(P0.05).Conclusion Both melatonin and cisplatin could inhibit the proliferation of lung cancer cells,the combination of them could produce a synergistic effect.
MTT assay
Cite
Citations (0)
Objective:To investigate the effect of rSIFN-co,consensus Interferon(Infergen),IFNα-2b(Interferon α-2b)and chemotherapeutics on cervical cancer cell line Caski in vitro.Methods:After Caski cervical cancer cells were cultured in vitro with rSIFN-co、Infergen、IFNα-2b and cisplatin(DDP)at the concentration of 0.156 μg/ml、0.625 μg/ml、2.5 μg/ml and 10 μg/ml respectively for 72 hours,cell proliferation was mensurated by MTT assay,cell inhibition rate was calculated.Then after Caski cervical cancer cells were treated with rSIFN-co、Infergen、IFNα-2b and DDP at the concentration of 0.156 μg/ml、0.625 μg/ml、2.5 μg/ml for 72 hours,cell apoptosis was determined by flow cytometry.Results:rSIFN-co、Infergen、IFNα-2b and DDP could induce inhibition and apoptosis of Caski cells.The inhibition and apoptosis of Caski cells which had been induced by rSIFN-co and DDP were better than which had been induced by Infergen and IFNα-2b at the same concentration,and DDP was more better than rSIFN-co.Conclusions:rSIFN-co has antineoplastic effect by inhibiting proliferation and inducing apoptosis in Caski cervical cancer cell in vitro.The effect is inferior to DDP,but better than the same type interferon and general type I interferon.
MTT assay
Growth inhibition
Cite
Citations (0)
To investigate the effect and mechanism of the selective COX-2 inhibitor NS-398 and carboplatin on the human cervical carcinoma cell line Hela.The effect of NS-398, carboplatin, and both on the proliferation of Hela cells was assessed by methyl-thiazolyl tetrazolium (MTT) method, and the apoptosis assay and cell cycle distribution were analyzed by flow cytometry.NS-398, and carboplatin inhibited the growth of Hela cells in a dose and time-dependent manner. When combining carboplatin with NS-398, the combined inhibition rate was increased, which nearly equaled the inhibition rate of the double concentrations of carboplatin. Flow cytometry demonstrated that the cell cycle was redistributed: the G(1)-phase cell fraction was increased while the S-phase cell fraction was significantly decreased after the cells were treated with NS-398 (P<0.05), However,the result was just the opposite after being treated with carboplatin. The apoptotic rate was 1.48%+/-0.03% and 3.43%+/-0.02% for pre-treatment and post-treatment with NS-398 respectively (P>0.05) while the apoptotic rate was 9.32%+/-0.02% after the treatment with carboplatin (P<0.05).NS-398 can inhibit the growth of Hela cells. The effect of NS-398 on Hela cells may not be related to the apoptosis. NS-398 and carboplatin can bring about synergistic effect in chemotherapy on Hela cells.
Carboplatin
HeLa
MTT assay
Cite
Citations (0)
PURPOSE: The aim of the study presented here was to evaluate the effect of ursodeoxycholic acid on viability and proliferation of cultured human tumor cells. MATERIALS AND METHODS: The following permanent cell lines were included as model systems in the experiments: MCF-7 (human breast cancer), HeLa (human cervical cancer) and A549 (human lung cancer). The effects on cell viabilituy and proliferation were studied by MTT test and colony-forming method. Statistical differences between control and treated groups were assessed by unpaired Student t-test and calculated by Graph-Pad Prism 4.0 software package. RESULTS: Applied at concentrations of 10, 50, 100 and 200 µg/mL for 24 h and 48 h, ursodeoxycholic acid (UDCA) decreased in a time- and concentration- dependent manner the viability of breast and lung cancer cells, while human cervical cancer cells remained almost unaffected. In the same concentration range (10-200 �g/ml), UDCA did not inhibit completely the ability of tumor cells to grow in a semisolid medium. CONCLUSION: Based on their sensitivity to the toxic effects of UDCA, the treated human tumor cell lines were graded as follows: MCF-7 > A549 > HeLa.
Ursodeoxycholic acid
HeLa
Viability assay
MTT assay
Cite
Citations (1)
Growth inhibition
Cite
Citations (42)
Combination chemotherapy is a crucial method in the treatment of gastric cancer. The aim of the present study was to investigate the inhibitory effects of puerarin and 5‑fluorouracil (5‑FU) on BGC‑823 gastric cancer cells in vitro and in vivo. The in vitro growth inhibition of puerarin or 5‑FU alone or combined on BGC‑823 cells was determined using a cell counting kit 8 (CCK‑8) on living cells. Apoptotic morphological features and proteins expression levels were detected by Hoechst 33258 staining, an Annexin V/propidium iodide apoptosis kit and western blot analysis, respectively. Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected. Results of the CCK‑8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments. The tumor volume was 90.65% of that of the controls and the mean tumor weight was only 0.125 g at the end of the experiment in the combination group compared with the control group (0.822 g). In addition, it was determined that liver and renal toxicity did not increase in combined treatment. These findings showed that puerarin and 5‑FU produced a significant synergic effect on gastric cancer cells, while there was no increase in side effects.
Puerarin
Propidium iodide
Cite
Citations (19)
1735 INTRODUCTION - Polysaccharide peptide (PSP) is a substance isolated from the deep-layer cultivated mycelia of Coriolus versicolor Yunzhi. It stimulates both humoral and cellular immune responses, and is also a valuable adjunct in raising the white blood cell counts of patients treated with chemotherapeutic drugs. In in vitro experiments, PSP has been revealed that it may have the ability to inhibit the proliferation of leukemia, lymphoma, stomach and lung cell lines. Paclitaxel (Taxol), another plant product, which is extracted from Taxus brevifolia, is an anti-neoplastic drug. It has shown to be a promising agent for the chemotherapy of ovarian, breast, and various cancers. In this study, the effects of PSP and Taxol upon the metabolic rate and apoptosis on breast cancer tissues were observed. MATERIALS AND METHOD - 39 patients’ tissues were treated with 4, 2 and 1 mg/mL of PSP and 4.27 ug/mL of Taxol for 24 hours. ATP Bioluminescence was used to measure the metabolic rate of the tissues. SPSS was to evaluate the results. SUMMARY - All results of PSP and Taxol compared with the control group had significant effects upon the suppression of tumor activity. In 4.27 μg/mL Taxol, 81.82% of the treated samples showed a percentage decrease in metabolic rate greater than 50% when compared to the control. For the same kind of calculation, 4 mg/mL of PSP is 48.72%, 2 mg/mL of PSP is 51.28% and 1 mg/mL of PSP is 46.15%. The comparison between the used drugs and tumor grading is detailed in table 1. PSP showed a higher percentage upon low-grade tumors. Our study has proven that PSP has a direct anti-tumor effect reflected by the amount of ATP measured. After the treatment of the two chemicals, tissues of both groups showed DNA fragmentation. CONCLUSION - PSP has similar effect in vitro on decreasing the activity, reflected by measuring the amount of ATP and apoptosis, of breast carcinoma solid tumor tissue compared to Taxol. PSP has a potent to be an orally neoadjuvant agent.
Cite
Citations (3)