Screening of Xerosis Inhibitor from Seaweed Extracts Using HaCaT Keratinocyte
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Abstract:
The primary function of the skin is to protect the body from the unwanted environmental influences. The outermost layer of the skin is stratum corneum which consists of corneocytes surrounded by lipid regions. Ceramides covalently bound to keratinocytes are essential for the barrier function of the skin, which can be disturbed in the disease, like xerosis. Xerosis is an abnormal dryness of the skin which reduced the thickness of stratum corneum and ceramide content decreasing with age. In this study, 36 seaweed extracts have been tested for screening of xerosis inhibitory agent by in vitro HaCaT keratinocyte assay. Ishige sinicola and Helminthocladia australis induced the significant amount of ceramide-like substance I in HaCaT keratinocyte among the tested seaweed extracts. Sargassum fulvellum, Chondrus ecellatus and Gigartina tenella also induced the ceramide-like substance I whereas Helminthocladia australis and Pachymeniopsis elliptica induced the ceramide-like II from HaCaT keratinocyte.Keywords:
HaCaT
Corneocyte
Barrier function
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Abstract Psoriasis is a skin disease associated with hyperproliferation and aberrant differentiation of keratinocytes. Our previous studies have identified the root of Rubia cordifolia L. as a potent antiproliferative and apoptogenic agent in cultured HaCaT cells (IC 50 1.4 μg/ml). In the present study, ethanolic extract of Radix Rubiae was fractioned sequentially with hexane, ethyl acetate (EA), n‐butanol and water. EA fraction was found to possess most potent antiproliferative action on HaCaT cells (IC 50 0.9 μg/ml). Mechanistic study revealed that EA fraction induced apoptosis on HaCaT cells, as it was capable of inducing apoptotic morphological changes. Annexin V‐PI staining assay also demonstrated that EA fraction significantly augmented HaCaT apoptosis. In addition, EA fraction decreased mitochondrial membrane potential in a concentration‐ and time‐dependent manner. The standardized EA fraction was formulated into topical gel and its keratinocyte‐modulating action was tested on mouse tail model. EA fraction dose‐dependently increased the number and thickness of granular layer and epidermal thickness on mouse tail skin, indicative of the keratinocyte differentiation‐inducing activity. Taking the in vitro and in vivo findings together, the present preclinical study confirms that EA fraction is a promising antipsoriatic agent warranting further development for psoriasis treatment. Copyright © 2009 John Wiley & Sons, Ltd.
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ABSTRACT Objectives ：This study was carried out to investigate anti-oxidative effect s of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte.Methods : Anti-oxidative effects were measured by estimating the amount o f total phenolics and flavonoids. In addition, DPPH free radical scavenging activitie s were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by h ydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200 ㎍/㎖. 42.64±1.90 ㎍/㎖ of total phenolics and 28.09±1.84 ㎍/㎖ of flavonoids was detected from TH ethanol extract. In addition, D PPH free radical scavenging activities of TH were elevated in dose-dependent manner. In add ition, The value of half maximal inhibitory concentration (IC 50 ) was 165.5 ㎍/㎖. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly.Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.Key words : Taraxaci Herba; anti-oxidative effect; HaCaT keratinocyte
HaCaT
Malondialdehyde
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