The comparison of urinalysis results at differences temperatures
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Objective: To study the differences among the results of urinalysis at differences.Methods: MA_4210 urinalysis system was used to detect urine samples at 4 degrees C,35 degrees C and room temperature.Results: Each factor,such as pH、GLU、PRO、SG and URO in urinalysis at light temperarure(35 degrees C) were significantly higher than those at room temperature and lower temperature (4 degrees C).Conclusion: There are significant differences among the main results in urinalysis at different temperatures.More attentions should be paid to it.Cite
This study evaluated results from an in-house (IH) laboratory dipstick, a reference laboratory (RL) urinalysis, and urine protein : creatinine (UPC) ratios from callimicos ( Callimico goeldii ). Urine was collected from 25 individuals comprising a single colony under professional care in North America and compared based on laboratory, sex, age class, and presence or absence of a normal urinalysis. Urine specific gravity and pH between laboratories were statistically different. Overall, 56% to 100% of animals had at least a trace amount of protein in their urine. In comparing normal and abnormal urinalyses, IH dipstick protein, RL dipstick protein, RL pH, quantitative protein measurement, and UPC ratios were all statistically different. Eleven animals (44%) had UPC ratios that were ≥0.5. Based on results of this study, UPC > 0.3 was found to be abnormal and supportive of renal compromise in callimicos. Higher protein concentrations on the IH dipstick, the quantitative protein concentration, and UPC ratio in the 1- to 4-yr-old age class were the only significant age-related differences. There was no association between any categorical variable (glucose, blood, bilirubin, ketones, urobilinogen) and abnormal urinalysis. There were no differences between sexes. Since renal disease is a major cause of morbidity and mortality in this species, the authors recommend including routine urinalyses and UPC ratios as part of preventive care programs for callimicos. These data provide the first published information on urinalysis and UPC ratios in callimicos and will serve as a helpful reference for interpreting results and evaluating patients with renal disease.
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Objective To investigate the sensitivities of Sysmex UF-1000i urine sediment analyzer(UF-1000i) in detecting urine red blood cells(RBC),white blood cells(WBC) and casts(CAST).Methods Urine specimens of 1 054 patients were collected,and each sample was examined by UF-1000i,URISys-2400 automatic dry chemistry analyzer and Diasys R/S2005 microscopy.The sensitivities of the 3 urine analysis methods were compared.Results The positive rates of urine RBC,WBC and CAST were 20.6%,20.3% and 6.7% respectively by UF-1000i.The positive rates of RBC and WBC were 24.5% and 17.9% by dry chemistry analyzer respectively.The positive rates of RBC,WBC and CAST were 16.9%,20.5% and 2.2% respectively by urine sediment microscopy.The results were statistically analyzed by χ2 test.The detection rates of RBC and CAST were significantly different(P0.01),but the detection rate of WBC did not differ significantly(P 0.05).Conclusions Urine sediment microscopy can not be completely replaced by UF-1000i in urinalysis.The combination of these 3 methods would be optimal to improve the accuracy and precision in urinalysis.
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Objective To investigate the positive rate of abnormal results of urinalysis among healthy population in Xi′an.Methods To carry out dry chemical analysis of urine samples in 6 000 cases and classify the results.Results The positive urine detected rate(±-++++) in 6 000 cases ranked as followed:white blood cells,blood,protein,bilirubin,ketones,sugar,nitrite.The weak positive(± or trace) urine detected rate ranked as followed:blood,white blood cells,ketones,protein,bilirubin,besides,glucose and nitrite did not find weak positive.A total of 1 023 items were positive,and 879 items were weak positive.In the test project,the white blood cells,blood,protein,nitrite and other positive rates were significantly different in various sex and age(P0.05).Conclusion Urine analysis is an indispensable test for the screening and early detection of disease of the healthy people.
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Objective To evaluate the reliability of dry urinalysis in the examination of urinary sediment.Methods The urinary specimen of 1000 patients were separately determined by urinary sediment microscopy and dry urinalysis to observe the sensitivity,specificity,false positive rate and false-negative rate of dry urinalysis.Results The dry chemical analysis of the examination of RBC revealed the sensitivity of 68.3%,and the specificity of 87.61%,the false-positive rate of 12.4% and the false negative rate of 31.68%.The dry chemical analysis of the examination of WBC revealed the sensitivity of 67.4%,the specificity of 84.02%,the false-positive rate of 16%,and the false negative rate of 35.26%.Nitrite: The dry chemical method and the urinary sediment microscopy both revealed 73 positive cases,with the coincidence rate being 100%.Conclusion Dry urinalysis has a higher specificity.
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Concordance
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A recent study in young infants found that different cutoffs maximized the accuracy of the urine white blood cell count in dilute versus concentrated urine samples. We aimed to confirm this finding and to determine its impact on clinical care.We conducted a retrospective analysis of data gathered on consecutive children <24 months of age with visits to the emergency department during a 5-year period. We evaluated the accuracy of screening tests for urinary tract infection (UTI) in dilute and concentrated urine samples. We also calculated the number of children who would have been treated differently in a hypothetical cohort of 1000 children presenting with fever had urine specific gravity (SG) been taken into consideration.We included 10 078 children. The ability to rule in UTI (as measured by the positive likelihood ratio [LR]) was similar in dilute and concentrated urine for the leukocyte esterase test (11.76 vs 10.71, respectively). The positive LR for urine white blood cell count per high-powered field was higher in dilute urine (9.83 vs 6.12). In contrast, the positive LR for the nitrite test was lower in dilute urine (20.54 vs 47.44). Despite these differences, we found little change in the number of children treated with antibiotics in predictive models that took urine SG into consideration.Although we found that urine SG influences the accuracy of some components of the urinalysis, its inclusion in the decision-making process had negligible effect on the clinical care of children with UTI.
Leukocyte esterase
Urine specific gravity
White blood cell
High-power field
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