A Multiplex PCR Method to Detect the Vibrio parahaemolyticus Strains
0
Citation
0
Reference
20
Related Paper
Keywords:
Thermolabile
Hemolysin
Vibrio Infections
Cite
One hundred and ninety-six Vibrio parahaemolyticus strains that are etiological agents causing outbreaks and sporadic cases of food toxicoinfections and isolated from the environments of Russia, Ukraine, and Turkmenia are characterized by virulence-associated phenotypic and genotypic signs. Most clinical strains were found to contain the genes of the major pathogenicity factors - thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH); however, a large number of pathogens lack these genes. In all likelihood, these strains can cause human diseases, by producing other toxic substances. The presence of the trh gene correlated with that and expression of the urease genes. Comparative analysis of the findings has demonstrated that the results of polymerase chain reaction detection of the tdh gene do not always agree with the signs of hemolytic activity on the Wagatsum medium (Kanagawa phenomenon). Therefore, both tests that complement each other are recommended for a fuller evaluation of the potential danger of Vibrio parahaemolyticus strains.
Hemolysin
Vibrio Infections
Cite
Citations (1)
Thermolabile
Hemolysin
Cloning (programming)
Cite
Citations (0)
Hemolysin
Cite
Citations (0)
Objective To analyze the serotypes,thermostable direct hemolysin and drug resistance of Vibrio parahaemolyticus isolated in Jianggan. Methods Serotypes of Vibrio parahaemolyticus were determined by serum agglutination test.Real-time PCR was used for the detection of thermostable direct hemolysin,and drug resistance test for 11 antibiotics was performed by disc diffusion method.Chi-square test was applied for data statistical analysis. Results Serotype 03 and 04were found to be dominant among strains isolated from samples of food poisoning and gastrointestinal outpatient,while there was no dominant serotype found in aquatic products.The detection rates of thermostable direct hemolysin from samples of food poisoning and other gastrointestinal outpatient were all over 90% without statistical difference(χ2=0.03,ν=1,P=0.86).No thermostable direct hemolysin was detected in bacteria strains isolated from aquatic products.62 strains were 100%sensitive to chloramphenicol,tetracycline and SMZ-TMP. Conclusions Serotypes and thermostable direct hemolysin are different among Vibrio parahaemolyticus strains isolated from food poisoning,other gastrointestinal outpatients and aquatic products.Ideal antibiotics for treatment of vibrio parahaemolyticus are chloramphenicol,tetracycline and SMZ-TMP.
Hemolysin
Cite
Citations (0)
Objective:To investigate the virulence gene change of Vibrio parahaemolyticus from 2006 to 2008 in Dongguan.Methods:PCR method and Kanagaw phenomenon method was used to detect the virulence gene.Results:The positive rate of tdh and trh gene in the Vibrio parahaemolyticus strains from food poisoning samples was 85.2%(127/149) and 8.7%(13/149),respectively.And it was 6.3%(3/48) and 2.1%(1/48) from the environment samples.The positive rate of Kanagaw phenomenon for all the Vibrio parahaemolyticus strains was 91.9%(137/149).Conclusion:The pathogenicity of Vibrio parahaemolyticus strains in Dongguan is very strong,and most of them from food poisoning include tdh gene.It is necessary to strengthen the surveillance of Vibrio parahaemolyticus.
Food poisoning
Cite
Citations (0)
Objective:To control contamination of Vibrio parahaemolyticus is often hampered by the lack of standardized methods and by the uncertainty associated with biochemical identification of the isolates.In this study,Vibrio parahaemolyticus was be examined thermolabile hemolysin(TLH) gene.Methods:PCR method was used to examine TLH gene isolates from clinical samples.Results:The TLH gene was found in all isolates.Conclusion:TLH gene has some value in detecting Vibrio parahaemolyticus.
Thermolabile
Hemolysin
Cite
Citations (0)
Vibrio parahaemolyticus is a marine bacterium which is the leading cause of bacterial gastroenteritis due to consumption of raw or undercooked bivalve molluscan shellfish in United States and is considered an important seafood-borne pathogen throughout the world. The conventional methods for enumerating this bacteria from seafood is laborious and time consuming, hence the objective of our study was to develop antibody based method which would be sensitive, user-friendly and less time consuming to detect and enumerate Vibrio parahaemolyticus from oysters. In order to condense time and material two rapid and specific antibody based test methods were developed. In the first method, monoclonal antibodies were produced against the polar flagella of Vibrio parahaemolyticus. The monoclonal antibody was employed in Immunomagnetic separation (IMS) protocol and was then coupled with Real-time PCR (q-PCR). IMS coupled to q-PCR was able to detect bacterial numbers in less than 3 hrs with a sensitivity of 1,000 CFU/g of oyster homogenate. In the second method anti-hemolysin serum was produced and employed in Direct colony immunoblot (DCI) protocol to detect pathogenic Vibrio parahaemolyticus. DCI when compared with the currently used conventional method, DNA hybridization (DNAH) showed good correlation and was a faster and simpler method requiring less equipment and stringent laboratory conditions than DNAH. From our results we have found that IMS+q-PCR or DCI could be an useful addition to the methods available for enumerating pathogenic V. parahaemolyticus in seafood.
Hemolysin
Immunomagnetic separation
Pathogenic bacteria
Ostreidae
Cite
Citations (0)
Objective To understand the serotype and virulence genes of Vibrio parahaemolyticus isolated from patients of foodborne diseases and environment in Shenzhen. Methods There 133 Vibrio parahaemolyticus strains were isolated from patients of foodborne diseases and environment (such as the sea water and seafoods)during the years of 2006~2008, and were serotyped according to GB/T4789.7 -2008. Thermostable direct hemolysin gene (tdh) and thermostable direct hemolysin-related hemolysin gene(trh) wre also determined by PCR. Results O3(43.6%),O1(16.5%),O4(16.5%) were predominant serogroups. 84.4% (76/90)foodborne disease isolates carried tdh gene,and none carried trh gene only. 79.1%(34/43)environmental strains carried neither tdh nor trh,and none carried tdh and trh simultaneously. Conclusion In recent years,Vibrio parahaemolyticus causing foodborne disease carries tdh (+) and trh (-),and a majority of environmental isolates carry tdh(-)and trh(-)in Shenzhen.O3 was still the predominant serogroup.
Hemolysin
Cite
Citations (0)
Hemolysin
Cite
Citations (23)
Thermolabile
Hemolysin
Cite
Citations (23)