The expression and significance of tumor-associated antigen RCAS1 in non-small cell lung carcinoma
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Objective To explore the relationship between the expression of tumor-associated antigen RCAS1(receptor-binding cancer antigen expressed on SiSo cells) and the clinical pathological factors in non-small cell lung carcinoma. Methods Immunohistochemical SABC method was used to detect the expression of RCAS1 in 61 non-small cell lung carcinoma tissues, 10 benign lung tissues and 10 normal lung tissues. The relationship between the expression and clinical pathologic charactors was analyzed. Results The positive rate of RCAS1 in non-small lung carcinoma was significantly higher than that in normal lung tissues (P 0.05). But there was no significant difference between the expression or rate of RCAS1 in non-small cell lung carcinoma and benign lung tissues (P 0.05). RCAS1 expression in non-small cell lung carcinoma was correlated with tumor differentiation and clinical staging (P 0.05). Conclusions RCAS1 may participate in the onset and progression of non-small cell lung carcinoma. RCAS1 may be a new biomarker in the diagnosis, treatment and prognostic evaluation of non-small cell lung carcinoma.Keywords:
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Objective:To investigate p63 、 TTF-1 expression and the value of the differential diagnoses in different tissue types of lung cancer . Methods: Using S-P immunohistochemical methods, we examined the expression of p63 and TTF-1 in 116 cases of primary lung cancer. Results: The positive rate of p63 was 100 (47/47) in primary lung squamous cell carcinoma . In contrast, other histologic types of lung cancer were negative or low expression.The positive rates of TTF-1 expression were 93.3 (14/15) and 93.5 (43/46) respectively in small cell carcinomas and adenocarcinomas of lung . In contrast, lung squamous cell carcinoma was negative. Conclusion: p63 and TTF-1 may be a useful immunohistochemical marker for lung squamous cell carcinoma and small cell carcinoma,adenocarcinoma of lung respectively . Combined examination of p63 and TTF-1 is valuable in the differential diagnosis in different histologic types of lung cancer . Especially it may be as a marker in distinguishing poorly differentiated squamous carcinoma,small cell carcinoma and adenocarcinoma of lung .
Large cell
Squamous carcinoma
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Objective: To explore the expression and the clinical significance of MAGE-A10 in human lung cancer. Methods: By using tissue array and S-P immunohistochemical methods with anti-MAGE-A10 monoclonal antibody, the expression of MAGE-A10 protein in 65 cases lung cancer was investigated and the correlation of MAGE-A10 expression with histological types and pathological grades was also evaluated. Results: The positive expression rates of MAGE-A10 were 36.4% in small cell lung carcinoma, 76.5% in squamous cell lung carcinoma and 40.0% in lung adenocarcinoma repectively, but no positive case can be found in large cell lung carcinoma and bronchioloalveolar carcinoma. It was no statistical significance between the expression ratio of MAGE-A10 protein in the small cell lung carcinoma(SCLC) and non-small cell lung carcinoma(NSCLC). The correlation of pathological grades in both the squamous cell carcinoma groups and the adenocarcinoma groups with expression of MAGE-A10 was also not been found. Conclusion: The expression ratio of MAGE-A10 in squamous cell lung carcinoma was the highest, followed by that in lung adenocarcinoma and SCLC suggesting that MAGE-A10 might be used as an useful marker in pathological diagnosis as well as a potential target in biological therapy of lung cancer.
Clinical Significance
Large cell
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Objective To investigate the expression and diagnostic significance of thyroid transcription factor1( TTF-1) in lung cancer tissues. Methods The expression of TTF-1 was detected by immunohistochemical staining in 50 cases of primary lung cancer( including 23 cases of adenocarcinoma,14 cases of squamous carcinoma,9 cases of small-cell lung cancer,and 4 cases of large-cell lung cancer) and para-carcinoma tissues as well as 21 cases of metastatic lung adenocarcinomas from digestive tract and ovaries. The diagnostic and differential diagnostic values of TTF-1 in lung cancer were assessed. Results The positive expression rates of TTF-1 were 73. 9%( 17 /23) in adenocarcinoma,0 in squamouse cell carcinoma,77. 8%( 7 /9) in small-cell lung cancer tissues and 50. 0%( 2 /4) in large-cell lung cancer tissues,0 in metastatic lung adenocarcinomas,respectively. The positive expression rates of TTF-1 in adenocarcinoma and small-cell lung cancer tissues were higher than that in squamouse cell carcinoma tissues( P 0. 05). In para-carcinoma tissues of primary lung cancer,normal or proliferous alveolar epithelium were less staining and had fewer positive cells( 20%). The expressions of TTF-1 were all negative in the para-carcinoma tissues of secondary lung cancer. The positive expression rate of TTF-1 in lung cancer tissues wasn' t related with patients' age,gender,tumor size,tumor location( P 0. 05). Conclusion TTF-1 can be used as a specific indicator for the differential diagnosis of primary or secondary lung adenocarcinomas,and it provides some references for the judgement of histological type of primary lung cancer.
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Objective To investigate the expressions of p53 family new members:p63 and p73 and their clinical significances in non small cell lung cancer (NSCLC).Methods Immunohistochemistry was used to detect p63 and p73 protein expressions in 60 NSCLCs and 7 normal lung tissues.Results In NSCLC,positive rates of p63 and p73 protein were 80 0%(48/60),73 3%(44/60) respectively.There were significant differences of p63 and p73 protein compared with normal lung tissue, P 0 05.Expression of p63 protein was closely associated with lymph node metastasis ( P =0 028) and histology types,P=0 000 3,but was not associated with cellular differentiation degree and clinical stages, P 0.05.There was no correlation among p73 protein expression and histology types,lymph node metastasis,cellular differentiation degree and clinical stages, P 0 05.A positive correlation was present between p63 and p73 protein expressions, P =0 000 1.Conclusion The elevated p63 and p73 protein may cooperate to prompt formation of lung cancer,p63 gene is a malignant progress marker of lung squamous cell carcinoma.
Histology
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Objective To study the clinicopathological significance of the expression of GSTP1 protein in lung cancer.Methods The expression of GSTP1 protein was detected by immunohistochemistry in 50 cases of primary lung adenocarcinoma specimens,20 adjacent noncancerous lung tissues,11 metastatic pulmonary adenocarcinoma and 14 squamous cell carcinoma tissues.Western blot was used to examine the GSTP1 protein level in 8 cases of lung adenocarcinoma,squamous cell carcinoma and adjuvant non-cancerous tissues.The relationship between the protein expression and clinicopathological features was analyzed.Results Overexpression of GSTP1 protein was shown in 32 and 9 cases of lung adenocarcinoma and squamous cell carcinoma tissues,respectively,which were much higher than that in adjuvant noncancerous lung tissues(χ2=6.655,P =0.010;χ2=3.927,P =0.048),but there was no statistically significant difference of GSTP1 expression between primary lung adenocarcinoma,metastatic pulmonary adenocarcinoma and squamous cell carcinoma tissues(P 0.05).The expression of GSTP1 protein was not correlated with age,sex,tumor size,differentiation or lymph node metastasis(P 0.05),but had a negative association with the prognosis of the lung adenocarcinoma patients(χ2=4.171,P =0.041).The expression of GSTP1 protein was higher in lung adenocarcinoma and squamous cell carcinoma tissues than that in non-cancerous lung tissues detected by Western blot(t =2.545,P = 0.023;t =2.337,P =0.035).Conclusion GSTP1 is up-regulated in lung cancer and positively correlated with the prognosis of the lung adenocarcinoma patients.
GSTP1
Squamous carcinoma
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Objective:To investigate the expression of C-myc and PCNA(proliferating cell nuclear antigen) in lung cancer and normal lung tissue or lung tissue of bullae and the relationship to the development of lung cancer.Methods: Immunohistochemical staining was used to measure expression of C-myc and PCNA in 50 patients with primary lung cancer(squamous cell carcinoma in 24 cases,19 cases of adenocarcinoma,adenosquamous carcinoma in 4 cases,3 cases of small cell lung cancer) and 16 normal lung tissues or lung bullae tissue.Results: The expression of C-myc,PCNA positive rates were 66%(33/50),74%(37/50) in total of 50 cases of primary lung cancer,no expression of two antibodies observed in normal tissue.The expression of C-myc and PCNA positive rate was significantly higher in lung cancer than lung tissue,and there was no expression of C-myc in 3 cases of small cell lung cancer.Conclusion: C-myc expression in lung cancer was no correlation to PCNA;increased expression of C-myc and PCNA which was developed in the early stage in lung cancer had no association with the staging of lung cancer;they may be related to the development of lung cancer and are helpful to the early diagnosis and molecular targeting therapy.
Adenosquamous carcinoma
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【Objective】To determine the expression of HSP27 (heat shock protein 27, a member of heat shock protein family) in lung carcinoma and investigate the relationship between HSP27 expression and invasion of lung carcinoma. 【Methods】 Immunohistochemical staining was used to detect the expression of HSP27 in 63 cases of lung carcinoma, 21 cases of benign tumor and 22 cases of normal lung tissue to analyze the relationship between their expression and various clinic pathologic factors of lung carcinoma. 【Results】None of the normal intestinal tissues and benign tumor were HSP27 positive. In 63 lung carcinoma specimens, the positive expression rate of HSP27 was 63.5% (40/63). HSP27 expression correlated with the degree of tumor differentiation and tumor metastasis (P 0.05). But not related to some clinic pathological characteristics such as the age, sex and size. 【Conclusions】The expression of HSP27 in tumor tissue may be a new marker of the biological behavior of lung carcinoma.
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Objective: To investigate the expression and clinical significance of p27 in no small cell lung cancer(NSCLC).Methods:111 paraffin-embedded NSCLC samples were composed and 30 of them included both the tumor and adjacent lung tissue.The expression of p27 was examined with SABC immunohistochemical techniques.Result:The positive expression rate of p27 in tumor lung tissue was more lower than in the adjacent non-tumor lung tissue,with a significant difference.The expression of p27 had no relationship with clinicopathological factor Nsuch as sex,age,tumor size,histological type and TNM stage,but had relationship with differentiation of tumor lung tissue,lymph node metastasis,and prognosis.Conclusion:The expression of p27 protein is low in NSCLC.
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Objective To investigate the expression of VEGF-C in human non-small cell lung cancer and its clinical implication.Methods Immunohistochemical technique was used to detect the expression of VEGF-C in 76 cases with lung cancer.Results There are not significantly difference among different tissue types,and there is not correlation between well differentiated and poor differentiated.The expression of VEGF-C in stage Ⅱ lung cancer was more than stageⅠlung cancer.The VEGF-C positive rate was significantly higher in lung cancer with lymph nodes metastasis than that without. The four-year survival rate of the patients with positive expression of VEGF-C was significantly lower than those with negative expression.Conclusion Expression level of VEGF-C correlated well with lymph node metastasis, tumor progression and prognosis of non-small cell lung cancer.
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Objective: To analyze the relationship of Stat3 expression with clinical stages, tissue types, p53 and proliferation cell nuclear antigen (PCNA) in human lung carcinoma, and to evaluate the role of Stat3 in the pathogenesis of lung carcinoma. Methods: Immunohistochemical method were used to detected Stat3, p53 and PCNA in different tissues of patients (n = 42) with lung carcinoma who accepted neither radiotherapy nor chemotherapy. Results: The positive rate of StatS was 81. 0% in lung carcinoma and its expression level was related to the tissue type but not to T, N or the clinical stage. The expression level of Stat3 in non-small cell lung carcinoma (NSCLC) was higher than that in small cell lung carcinoma (SCLC). A positive correlation of the expression of Stat3 with that of p53 and PCNA was identified. Conclusion:The expression level of Stat3 is abnormal in lung carcinoma. Stat3 may be involved in the regulation of p53 gene in lung carcinoma cell, it may accelerate the proliferation of lung carcinoma cells and play an important role in the pathogenesis of lung carcinoma.
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