The determination of amino acid in Asparagus officinalis Leaf
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Abstract:
By using HPLC ,the content of 15 kinds amino acid in asparagus officinalis leaf was determinatedThe test result shown that the asparagus officinalis leaf contains many knrds of amino acid and was a excellent healthful drinking.Keywords:
Asparagus
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An antifungal saponin was isolated from the bottom cut of white asparagus (Asparagus officinalis L), which is unusable in food processing. The structure of the saponin was identified as 3-O-[{α-L-rhamnopyranosyl (1→2)} {α-L-rhamnopyranosyl (1→4)}-β-D-glucopyranosyl] (25S) spirost-5-ene-3β-ol from chemical and spectral data. This saponin was shown to be identical with collettinside III from Dioscorea collettii, and to inhibit the growth of some kinds of fungi at μg ml−1 levels.
Asparagus
Liliaceae
Asparagus racemosus
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Asparagus
Phytotoxicity
Allelopathy
Lepidium Sativum
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Asparagus is a popular vegetable rich in healthy functional components. Asparagus spears are known to contain a large amount of rutin, which has been found to possess anti-inflammatory, antitumor, and antibacterial/viral properties, and protodioscin, which is an antitumor substance and present in the bottom parts (8 cm from the cut end). However, the process of its production leaves fern in the aboveground parts and roots in the underground parts as significant amounts of nonusable parts, and this issue should be solved. This study was conducted to examine the distributions of rutin and protodioscin, representative functional components in different parts of asparagus. The results suggested that large amounts of rutin were noted in the cladophylls and storage roots (brown and epidermis), and the protodioscin content was high in the buds, the soil-covered section of the spear, and the rhizome. A significant amount of rutin was detected in the aboveground parts, which is consistent with the results of previous studies, but it was also found in the storage roots. The largest amount of protodioscin was found in the buds, as well as in young fruits and seeds of the aboveground parts. Injury by continuously cropping asparagus may be associated with high rutin content in the storage roots of asparagus.
Asparagus
Coleus
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The total flavone in plants of 7 different varieties of Asparagus officinalis L.were analyzed.The studies indicated that A1,A5 and A3 the contents of total flavone in Asparagus Spears were higher;A5、A2 and A7 the contents of total flavone in the leaves were higher;A7,A2 and A6 the contents of total flavone in roots were higher.Carried out the contrasts analyses on flavone contents of asparagus plants from different section,the highest contents of total flavone in leaves and the lowest contents of total flavone in roots of asparagus.
Asparagus
Flavones
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Liquid shoot culture of <em>Salvia officinalis</em> L. in MS medium containing IAA (0.1 mg l<sup>-1</sup>) and BAP (0.45 mg l<sup>-1</sup>) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 pg l<sup>-1</sup>) was added to the liquid medium. In optimum conditions (at 20 pg l<sup>-1</sup> TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of <em>S. officinalis</em>) (10-12 mg g<sup>-1</sup> dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g<sup>-1</sup> dry wt) was even 24 times higher compared to samples of dried leaves of <em>S. officinalis</em> plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from <em>S. officinalis</em>.
Carnosic acid
Rosmarinic acid
Triacontanol
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Asparagus
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Citations (54)
Asparagus
Liliaceae
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Citations (2)
Asparagus
Sapogenin
Diosgenin
Liliaceae
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The raw material base of medicinal plants in Ukraine is sufficient only for half of the pharmacopoeial species. Most part of the medicinal plants is growing in insufficient quantities and there is a need for their import. In conditions of import dependence and a shortage of domestic plant materials, the search for new sources of biologically active substances among the representatives of the flora of Ukraine is an urgent task of modern pharmaceutical science.
The aim of the work – Conduct a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis to establish the possibility of using the non-pharmacopeia species in pharmaceutical and medical practice.
Materials and methods – The objects of the study are the leaves of S. grandiflora and S. officinalis, which was harvested in the botanical garden of the Lviv National University named after I. Franko. The study of macro- and microelement composition in the leaves of S. officinalis and S. grandiflora was carried out by atomic emission spectrographic method. Determination of the qualitative composition and quantitative content of the main groups of biologically active substances was carried out by HPLC. Quantitative determination of phenolic compounds was also carried out by spectrophotometric method.
Results – The content of 15 micro and macro elements was found in both studied species. In the leaves of S. officinalis and S. grandiflora, 15 amino acids and 8 saponins were identified. Using HPLC, the qualitative composition and quantitative content of phenolic substances in the leaves of S. officinalis and S. grandiflora (13 and 9 compounds, respectively) was established.
Discussion – The dominant macro and micro elements in the studied species were silicon, phosphorus, magnesium, calcium, sodium, and potassium. The total content of trace elements in the leaves of S. grandiflora is 1.67 times greater than in the pharmacopeia plant S. officinalis. The dominant amino acids in the leaves of both species are glutamic acid, aspartic acid, valine and leucine.
The dominant saponins in the leaf of S. officinalis were ursolic and oleanolic acids, the total content of which is 75.82 %. In the leaves of S.grandiflora, ursolic and euskapic acids were dominant, with a total content of 63.25 %.
The total flavonoid content is higher in S. officinalis leaf and is 4.90 mg / g. The total content of hydroxycinnamic acids is highest in the leaf of S. grandiflora and is 4.49 mg / g, which is 221.18 % (2.21 times) higher than in the pharmacopeia plant S. officinalis (2.03 mg / g). The total highest content of caffeic acid derivatives prevails in the Salvia officinalis leaf (0.77 mg / g). The highest content of the sum of all detected phenolic compounds is specified for S. officinalis leaves and amounts to 6.93 mg / g.
Conclusions – As a result of a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis, it was established that S. grandiflora is a promising species for introduction into medical and pharmaceutical practice, namely, as a source of phenolic compounds.
Salvia officinalis
Phytochemical
Melissa Officinalis
Economic shortage
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A B S T R A C T Background and Objectives: The present study was conducted to evaluate the presence of biochemical contents and trace elements, and detect phytochemicals in the edible part extracts of Asparagus officinalis DC and Chlorophytum comosum Linn by HPTLC. Materials and Methods: The biochemical contents and trace elements were determined by different biochemical methods, and trace elements’ presence was detected by atomic absorption spectroscopy (AAS), while phytochemicals were detected by HPTLC. Nutrients content of the leaves of Asparagus officinalis DC and Chlorophytum comosum Linn were analyzed by standard method. Results: Crude fat and protein content of Asparagus officinalis DC and Chlorophytum comosum Linn was found to be 3.44, 2.00 and 32.69, 4.54%, respectively. Also their total carbohydrate and crude fiber content was detected as 34.67, 65.84 and 18.5, 17.24%, respectively. Analysis of the edible parts confirmed the presence of phenol steroids, and showed minimum amount of trace elements with moderate nutritive value. Total phenolic content and nutritive value were found to be greater in the stem Asparagus officinalis DC as compared to Chlorophytum comosum Linn. Conclusions: Our findings suggest that both Asparagus officinalis DC and Chlorophytum comosum Linn are endowed with antioxidant phytochemicals and nutritive values.
Profiling (computer programming)
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