Abstract:
Different explants from superior Eucommia clone No.2 were cultured to regenerate the mother plant rapidly by clone. The result are as follows: MS+6 BA 1.0 +NAA 0.2 +sucrose 4% was the optimum axillary sprout inducing medium, on which 82.5% axillary buds shot, apex sprout inducing medium: MS+6 BA 1.0 , on which 87.5% stem apex shot; plantlet growth medium: MS+IAA 1.0 +6 BA 1.0 ; rooting medium: 1/2MS+GA 0.5 +sucrose 6%, rooting rate arrived 20.7%.Keywords:
Apex (geometry)
clone (Java method)
Plantlet
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The study aimed to explore different tissue culture conditions of L.chuanxiong and optimize its induction and differentiation medium.In tissue culture with the root,stem segments and leaf of L.chuanxiong as explants,a lot of plantlets were obtained and the corresponding plant regeneration system was established.The optimum medium for inducing callus with L.chuanxiong root was MS+6-BA 0.8 mg/L+ NAA 1.2 mg/L and that with L.chuanxiong stem segments and leaf was MS+6-BA 0.5 mg/L+NAA 1.5 mg/L.The optimum medium for differentiating adventitious buds from root callus was MS+6-BA 2.2 mg/L+IAA 0.3 mg/L and that form stem segments and leaf was MS+ KT 2.0 mg/L+IAA 0.5 mg/L.The callus,differentiating adventitious bud and rooting induction of root explants were 84%,86% and 87% respectively.The callus induction rates of stem segments and leaf were 92% and 96% and their differentiation rate and rooting rate of adventitious bud were 98% and 93%.The transplanting survival rate of obtained plantlets was 98% seedling after-training.The medium with optimizing hormone combination was put forward and the high-effective induction rate,differentiation rate and rooting rate were obtained.
Callus
Transplanting
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The effects of various hormone ratios on adventitious buds,callus inductions and differentiation were investigated using the cotyledon node,leaf blade and petiole of Viola prionantha as explants.Finally,aplant regeneration system via tissue culture was established.The results showed that the optimal medium for adventitious bud induction from the cotyledon node and petiole was MS+2.0mg/L 6-BA+0.1mg/L NAA,but the leaf blade was not suitable as an explant.Three kinds of explants could induce callus formation.The optimal medium for callus induction from the cotyledon node and petiole was MS+1.0mg/L 6-BA+0.5mg/L 2,4-D with the highest callus induction rate being 98.3%and 96.7%,respectively.The optimal medium for callus induction from the leaf blade was MS+1.0mg/L 6-BA+0.5mg/L 2,4-D+0.05mg/L NAA with the highest callus induction rate being 88.3%.The best medium for callus differentiation was MS+1.5mg/L 6-BA+0.1mg/L NAA with the highest callus differentiation rate being 100%.The optimal medium for the proliferation of adventitious buds was MS+1.5mg/L 6-BA+0.075mg/L NAA,the highest proliferation times being 4.68.The rooting rate of regenerated plants was up to 92.3% on half strength MS medium supplemented with 1.0mg/L 6-BA.Rooted plantlets were grown on mixture with perlite:sand:humus(1:2:2);survival rate was up to 100% with good growth.
Callus
Petiole (insect anatomy)
Cotyledon
Perlite
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The growing shoots of jujube variety Luzao 1 were used to study the bud induction and rapid propagation of test tube plantlets.The results showed that the latent bud of shoot stem segments could be induced to break on the induction medium,which was MS supplemented with 1 mg/L BA,0.2 mg/L IBA and 3% sucrose,and the bud break rate was 54.8%.On the same induction medium,adventitious bud could be induced to regenerate from the distal cut surface of stem section not touch medium,and the regeneration rate was 23.8%.The propagation of adventitious bud and latent bud was better on the medium of MS+2 mg/L BA+0.4 mg/L IAA+3%sucrose+6 g/L agar.The rooting rate of test tube plantlets was over 70% on the medium of 1/4MS+0.5 mg/L IBA+2%sucrose+6 g/L agar.
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Rapid propagation of Eucommia ulmoides was studied by using stem segment with axillary bud.The results were as follows:The explants taken from the middle and top of adult trees in April and May per year had best sprouting ability and primary growth.The germination rate reached above 90.0%,the elongation ratio reached above 80.0%.After buds sprouting were induced by MS+6-BA 0.10 mg·L~(-1),and buding plants were subcultured for multiplication by MS+6-BA 2.00 mg·L~(-1)+NAA 0.05mg·L~(-1),then induced rooting by 1/2MS+IBA 1.50 mg·L~(-1) sucrose 20.00 mg·L~(-1),the rooting rate reached 90.0%.
Sprouting
Eucommia ulmoides
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Plumular axis of Aquilaria sinensis were cultured on four media with different PGR (Plant Growth Regulators).It is found that the MS+BA0.1mg/L+NAA 0 01mg/L and 1/2MS+BA0 1mg/L+NAA 0 01mg/L media were good for the induction callus.The higher induction frequency for the sprouts when the stem segments were cultured in the 1/2MS+BA 2 mg/L+NAA 0 5 mg/L+sucrose 3%,but the vitrification shoots were caused easy by this medium.The sprouts growth better in 1/2MS+KT 2 mg/L+IAA 0 02 mg/L.It is benefit for the rooting when seeding soaking in NAA 1 000 mg/L.
Plantlet
Vitrification
Callus
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Optimization of regeneration system of Eucommia ulmoides was studied by using tender leaves as explants.The results showed that MS+NAA1.5 mg/L +BA2.0 mg/L had the best effects on induceing callus with a inducing rate of 95%,the best medium of callus growth was MS+NAA1.0 mg/L +BA2.0 mg/L with a conserve rate of 98.5%,the suitable medium for bud formation was MS medium added NAA0.5 mg/L,BA2.0 mg/L,with a differentiation rate of 40%,the optimum media for rooting is 1/2MS+IBA1.5 mg/L with 92.5% rooting ratio.
Eucommia ulmoides
Callus
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[Objective] The study aimed to establish the high efficient tissue-culture regeneration system for the leaf of Chrysanthemum nankingense.[Method] With the leaves of C.nankingense as explants and MS medium as basic medium,10 kinds of media were designed by adding various hormone into MS medium so as to research the effect of various hormone combination and concentrations on induction rate of adventitious bud and rooting of Chrysanthemum nankingense leaves,and screen the optimal medium for callus induction,bud induction and rooting.[Result] The leaf explants of asepsis seedlings were cultured on the optimum medium of MS+2.0 mg/L 6-BA +0.2 mg/L NAA for inducing callus for 15 d,which got the induction of 98.0%,and then the callus with less explant were cultured on optimal medium of MS+1.0 mg/L 6-BA+0.5 mg/L NAA for bud induction,which got the budding rate of 94.0%,afterward the regenerated buds with length of over 1cm were cultured on optimal rooting medium of MS+IBA 0.05 mg/L,which got rooting rate of 100%,finally the seedlings that rooted for over 25 d had survival rate of over 95% after hardening and transplanting.[Conclusion] In the experiment,the tissue-culture regeneration technique procedure for C.nankingense leaf was established.
Callus
Transplanting
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The effects of plant growth regulators on the adventitious bud proliferation and rooting in Camellia oleifera were studied by using new shoot(with buds) and leaf as explants to establish a fast reproductive system.And the effects of different medium on survival rate of transplanted tissue culture seedlings were also investigated.The results showed that:(1) The highest frequency of shoot formation was obtained when explants(stem with buds) were cultured on 1/2MS+NAA(0.5 mg/L) +6-BA(1.0 mg/L)+ZT(0.5 mg/L).The inducement rate of valid buds reached to 74.5%.The regenerated shoot from leaf was obtained when cultured on 1/2MS+TDZ(1.0 mg/L)+IAA(0.1 mg/L)+6-BA(2.0 mg/L) for 7 weeks.(2) Regenerated shoot were cut as several cluster or single plantlet and transferred on 1/2MS+6 BA(1.0 mg/L)+NAA(0.05 mg/L) for shoot propagation and growth,which the multiplication coefficient was more than 4.17.(3) 88.3% of rooting rate,3.72 cm of average root length,and 8 roots of average plantlet were observed when cultured in 1/2 MS for 50 days,after shoot was dipped in 1 g/L IBA for 5 second.(4) The rooted plantlets were acclimatized on mixture of peat and vermiculite(1:1),which survival rate was 82.5%.
Plantlet
Camellia oleifera
Vermiculite
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The shoots of Eucommia ulmoides Oliv.as explants,the MS as the basic madium supplemented with 2,4-D,NAA,IBA,KT,the callus induction and growth of E.ulmoides Oliv.were studied.The results showed that,calluses were induced 100 per cent when explants were cultured on MS medium supplemented with three auxins.0.5 mg/L 2,4-D,0.5-1.0 mg/L NAA,1.0 mg/L IBA was best for callus induction and growth.Combination of 2,4-D and BA or KT was worse than 2,4-D singly.Low concentrated BA and NAA or IBA combinations had better effect than singly,but high concentrated BA was bad.The best combination of callus induction and growth was 1.0 mg/L NAA+0.3-0.5 mg/L BA or 1.0 mg/L IBA+0.5 mg/L BA,whose calluses were growing quickly,glossy,suitable for subculture.KT was bad for callus induction and growth.
Callus
Eucommia ulmoides
Subculture (biology)
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[Objective] The study aimed to explore the method and condition for establishing the regeneration system of Euonymus japonicus cv CuZhi.[Method] With MS as basic medium,the tender leaves and axillary buds of CuZhi as materials,the media added 6-BA and NAA with different concentration proportions and the media added IAA with different concn.were set up to conduct the cultures of inducing adventitious buds,proliferation and rooting.[Result] MS could be as basic medium for the regeneration system of CuZhi.The combination of 6-BA and IAA was in favor of inducing calli and adventitious buds from leaves and axillary buds,the effect of medium MS+6-BA 1.0 mg/L+NAA 0.1 mg/L was best and the induction rate of axillary buds was up to 66.67%.The combination of 6-BA with higher concentration and NAA with lower concentration was in favor of the proliferation of clustered buds,the proliferation effect of medium MS+6-BA 1.0 mg/L+NAA 0.2 mg/L was more perfect and the proliferation rate was up to 328.57%.The optimum rooting effect could be obtained from medium MS + IAA 0.5 mg/L + 3% sucrose or 1/4MS + IAA 0.5 mg/L + 2% sucrose.[Conclusion] The establishment of the system provided reference basis for the rapid propagation of CuZhi seedlings.
Euonymus
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