Study on distributed patterns of scoparone and ayapin in Dendrobium species from Yunnan
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An HPLC method for determination of scoparone and ayapin was established for investigating the distributed patterns of scoparone and ayapin in 37 species of Dendrobium. The contents of scoparone and ayapin in varied collected samples were determined by the established HPLC method. The pseudo-bulbs sampled were collected according to different growth age of D. thyrsiflorum. The results showed that the contents of scoparone and ayapin were much differently distributed in species of Dendrobium. Only D. thyrsiflorum and D. densfilorum contained both scoparone and ayapin, the content decreased with the growth age. A fewer amount of ayapin was tested in D. loddigesii from Wenshan. The scoparone and ayapin were not determined in the rest species of Dendrobium. The method was concise, sensitive, accurate and reproducible. It could be applied to assay scoparone and ayapin in populations of herbal Dendrobium.Keywords:
Dendrobium
Dendrobium
Orchidaceae
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The commercial standards of Dendrobium medicinal materials in Yunnan are very complicated. 1. The principal commodities of these materials include: 1. Xi-fengdou (spiral Dendrobium): They are divided into three classes, i.e. 1, 2 and 3, but recently Diaolan-fengdou (rough spiral Dendrobium) was discovered. 2. Huangcao (yellow herb Dendrobium): They are classified as Xi-huangcao (slender Dendrobium), Cu-huangcao (thick Dendrobium), Bian-huangcao (oblet Dendrobium), Xiaogua-auangcao (littlemelon Dendrobium) and Xian-huangcao (freshly Dendrobium). 2. nonprincipal Huangcao commodities in Yunnan include: Cha-huangcao (branch Dendrobium), Xianggun-huangcao (stick Dendrobium) and Yougua-huangcao (melon Dendrobium).
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Abstract This study aims to assess the diversity and degree of grouping of several types of orchids in Indonesia. Laboratory work was carried out at the Laboratory of the Center for Horticulture and Tropical Studies IPB by analyzing the variability of orchids using RAPD. Plant material is taken from the orchid collection at the Bogor Botanical Gardens. The relationship between 9 orchids was analyzed using binary phenotype data and DNA band patterns as assessed by amplification of 2 random RAPD primers. The cluster analysis was carried out under the NTSYSpc version 2.02 program and the UPGMA SimQual function. The results showed genetic diversity of the genus Phaius and Dendrobium. Based on primers OPA7 and OPA9 with a similarity coefficient of 0.4 divided into 4 groups. Group I consisted of Phaius tankervilliae, Phaius montanus and Phaius callosus . Group II consisted of Dendrobium mirbelianum, Dendrobium lamellatum, Dendrobium liniale and Dendrobium biggibum . Group III consists of Dendrobium anosmum . Group IV consisted of Phaius ambionensis . The conclusion of this study is that there are 4 groups from the genus Phaius and Dendrobium based on the genetic marker RAPD.
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Orchidaceae
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In response to DNA damage, p53 undergoes post-translational modifications (including acetylation) that are critical for its transcriptional activity. However, the mechanism by which p53 acetylation is regulated is still unclear. Here, we describe an essential role for HLA-B-associated transcript 3 (Bat3)/Scythe in controlling the acetylation of p53 required for DNA damage responses. Depletion of Bat3 from human and mouse cells markedly impairs p53-mediated transactivation of its target genes Puma and p21 . Although DNA damage-induced phosphorylation, stabilization, and nuclear accumulation of p53 are not significantly affected by Bat3 depletion, p53 acetylation is almost completely abolished. Bat3 forms a complex with p300, and an increased amount of Bat3 enhances the recruitment of p53 to p300 and facilitates subsequent p53 acetylation. In contrast, Bat3-depleted cells show reduced p53–p300 complex formation and decreased p53 acetylation. Furthermore, consistent with our in vitro findings, thymocytes from Bat3-deficient mice exhibit reduced induction of puma and p21, and are resistant to DNA damage-induced apoptosis in vivo. Our data indicate that Bat3 is a novel and essential regulator of p53-mediated responses to genotoxic stress, and that Bat3 controls DNA damage-induced acetylation of p53.
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Dendrobium
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Taking nine Dendrobium that were introduced from Yunnan to Nanning as materials,morphological characteristics,phenological characteristics and planting adapiton characteristics were studied,aiming to rich local Dendrobium provenances,protect and use wild Dendrobium resources.The results showed that Dendrobium chrysotoxum,Dendrobium pendulum,Dendrobium wardianum,Dendrobium densiflorum,Dendrobium thyrsiflorum,Dendrobium primulinum and Dendrobium trigonopus could grow and blossom normally.Due to its high ornamental value,it were worth to be chosen as crossing parents for cultivating ornamental new varieties of Dendrobium.
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Ornamental plant
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HLA-B-associated transcript 3 (BAT3) was originally identified as one of the genes located within human major histocompatibility complex. It encodes a large proline-rich protein with unknown function. In this study, we found that a fragment of the BAT3 gene product interacts with a candidate tumor suppressor, DAN, in the yeast-based two-hybrid system. We cloned the full-length rat BAT3 cDNA from a fibroblast 3Y1 cDNA library. Our sequence analysis has demonstrated that rat BAT3 cDNA is 3617 nucleotides in length and encodes a full-length BAT3 (1098 amino acids) with an estimated molecular mass of 114,801 daltons, which displays an 87.4% identity with human BAT3. The deletion experiment revealed that the N-terminal region (amino acid residues 1-80) of DAN was required for the interaction with BAT3. Green fluorescent protein-tagged BAT3 was largely localized in the cytoplasm of COS cells. Northern hybridization showed that BAT3 mRNA was expressed in all the adult rat tissues examined but predominantly in testis. In addition, the level of BAT3 mRNA expression was more downregulated in some of the transformed cells, including v-mos- and v-Ha-ras-transformed 3Y1 cells, than in the parental cells.
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Dendrobium
Orchidaceae
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