[Functional organization of papova- and adenovirus DNA].
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Functional organization
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ABSTRACT Recent DNA sequence analysis indicates that rhesus rhadinovirus (RRV) is a member of the lymphotropic gamma-2 herpesvirus family. To determine if RRV is lymphotropic, peripheral blood mononuclear cells from naturally infected monkeys were separated by immunomagnetic bead depletion and analyzed for the presence of RRV by virus isolation and nested PCR. The recovery and consistent detection of RRV in the CD20 + -enriched fraction clearly demonstrates that B lymphocytes are a major site of virus persistence.
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Aedes albopictus
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Journal Article Complete sequence of VP2 gene of the bluetongue virus serotype 1 (BTV-1) Get access S. Yamaguchi, S. Yamaguchi 1Department of Environmental Health, University of Alabama, University StationBirmingham, AL 35294, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar A. Fukusho, A. Fukusho 1Department of Environmental Health, University of Alabama, University StationBirmingham, AL 35294, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar P. Roy P. Roy 1Department of Environmental Health, University of Alabama, University StationBirmingham, AL 35294, USA2NERC Institute of VirologyMansfield Road, Oxford OX1 3SR, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 6, 25 March 1988, Page 2725, https://doi.org/10.1093/nar/16.6.2725 Published: 25 March 1988 Article history Received: 17 February 1988 Published: 25 March 1988
Sequence (biology)
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Abstract We report on the microarray‐based in vitro evaluation of two libraries of DNA oligonucleotide sequences, designed in silico for applications in supramolecular self‐assembly, such as DNA computing and DNA‐based nanosciences. In this first study which is devoted to the comparison of sequence motif properties theoretically predicted with their performance in real‐life, the DNA‐directed immobilization (DDI) of proteins was used as an example of DNA‐based self‐assembly. Since DDI technologies, DNA computing, and DNA nanoconstruction essentially depend on similar prerequisites, in particular, large and uniform hybridization efficiencies combined with low nonspecific cross‐reactivity between individual sequences, we anticipate that the microarray approach demonstrated here will enable rapid evaluation of other DNA sequence libraries.
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During the propagation of A (H3N2) influenza virus in chick embryos, incorporation of 3H-thymidine into virions takes place, whereas no such incorporation occurs with Newcastle disease virus. Incorporation of 3H-thymidine is a result of DNA synthesis. This virion-associated DNA is present in cores obtained after treatment of virions with bromelain.
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The ganglia of rabbits infected with a relatively benign strain of herpesvirus (E-43) and challenged with either of two virulent neurotrophic strains (MP or McKrae) were found to be colonized only by the initial benign infecting strain. Primary infection with the E-43 strain resulted in milder disease when the animals were infected with MP or McKrae strains and also prevented colonization of the ganglion by these strains. Neutralization with anti-glycoprotein C, plaque morphology, cytopathic effects, reconstruction experiments, and restriction endonuclease analysis indicated that the virus recovered from the ganglion was the initial infecting E-43 strain; no traces of the challenging MP and McKrae strains were found. The challenging McKrae strain was shed for several weeks in a few animals, but the virus isolated from the trigeminal ganglia of these animals was the primary infecting E-43 strain. These results suggest that initial infection with a relatively benign strain of herpesvirus may prevent superinfection of the ganglion (but not necessarily the end organ) by highly virulent herpes simplex virus strains and could have significant implications in the consideration of immunization against this disease in humans.
Superinfection
Strain (injury)
Trigeminal ganglion
Simplexvirus
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We demonstrate that structural data on the protein-DNA complex can be used to predict DNA target sequences for regulatory proteins, and to quantitatively estimate the relationship between the structure and specificity in protein-DNA interactions.
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More than 200 cells were cloned from populations of mammalian cells persistently infected with Japanese encephalitis virus. Only four cloned cultures contained cells that had viral antigen measurable by immunofluorescence and that released infectious virus, yet all clones harbored virus-specific RNA. Superinfection of cloned cells with wild-type Japanese encephalitis virus did not produce cytopathic effects, but resulted in production of viral antigen and infectious virus in formerly nonproducing clones. Cocultivation of nonproducer clone cells with normally permissive cells did not induce virus production, nor did treatment of nonproducer clones with various inhibitors of DNA, RNA, or protein synthesis. It is suggested that the cloning procedure may have selected for a particular subpopulation of cells and that defective virus is also involved in establishment and maintenance of persistent infection.
clone (Java method)
Viral transformation
Superinfection
Viral Interference
Helper virus
Permissiveness
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Protein–DNA interaction
Sequence (biology)
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