Expressions and significances of HGF and c-Met in the malignant transformation of nasal inverted papilloma
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Objective To analysis the expressions of HGF and c-Met in different stages of intranasal inverted papilloma and try to explore the effects of HGF and c-Met in the malignant transformation of nasal inverted papilloma.Methods 30 cases with confirmed malignant papilloma and 55 cases with nasal inverted papilloma in our hospital from Jan 2005 to Dec 2010 were received as experimental objects.All the patients underwent endoscopic removal of diseased tissue.The expressions of HGF and c-Met in nasal inverted papilloma tissue,adjacent tissues and malignant tissues were determined by immunohistochemical method,and the stain score of each sample was calculated.The expressions of HGF and c-Met in different stages of nasal inverted papilloma were analyzed.Results The staining scores of HGF and c-Met in malignant tissue were higher than those in nasal inverted papilloma tissues and the adjacent tissues,the expressions of HGF and c-Met in the adjacent tissues were the minimum.Expressions of HGF and c-Met in T1 stage of inverted papilloma were significantly lower than those in T3 stage(P 0.05,P 0.01) and T4 stage(both P 0.01).Conclusions The expressions of HGF and c-Met in nasal inverted papilloma were related to its stage,and may contribute to the malignant transformation of it.Keywords:
Inverted Papilloma
Malignant Transformation
Stain
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Objective To investigate the expression of proliferating cell nuclear Antigen(PCNA) and assisted with the tension of protein and protein homology,the No.10 chromosome lost phosphatase gene(phosphatase and tensin homology deleted on chromosome ten,PTEN)in inverted papilloma of nasal cavities and paranasal sinuses and its carcinogenesis.Methods PCNA and PTEN protein expression in 55 paraffin tissue specimen of nasal cavities and paranasal sinuses,including inverted papilloma,atypical hyperplasia and papillary carcinoma were detected using immunohistochemical EnVision two step method.Results PCNA and PTEN protein were expressed in inverted papilloma,atypical hyperplasia and papillary carcinoma.The expression of PTEN protein showed a different distribution pattern to that of PCNA,the PCNA protein expression have a rising gradually and PTEN protein expression is decreasing gradually.Conclusions PCNA and PTEN protein expression change are closely related to the malignant transformation of inverted papilloma.PCNA expression up-regulated and loss of PTEN expressions may be a favorable marker and prompted prognosis in patient with inverted papilloma of nasal cavities and paranasal sinuses.
Inverted Papilloma
Tensin
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Objective: To investigate the expression of difference MMP-2 and MMP-9 of matrix metalloproteinases in nasal inverted papilloma(NIP). Methods: 30 NIP and 15 nasal polyps (NP) were studied with immunohistochemical LSAB (Labeled Streptavidim Biotin) method. Results: The results indicated that 56.67% cases of NIP were MMP-2 positive,and 80.00% cases of NIP were MMP-9 positive. Conclusion: MMP-2 and MMP-9 may play an important role in the development of NIP.
NIP
Inverted Papilloma
Nasal Polyps
Matrix (chemical analysis)
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Objective To explore the possible mechanisms underlying the malignant transformation of inverting papilloma through analyzing the expressive activities of proliferating cell nuclear antigen (PCNA), bcl-2 and p53 in the tissues of inverting papilloma (IP), IP accompanied with dysplasia and IP accompanied with malignant transformation respectively in nasal cavity and/or sinuses in a comparative way. Methods Included in this study were 31 cases of IP, 10 cases of IP with dysplasia and 5 cases of IP with malignant transformation to squamous cell carcinoma (SCC), all in nasal and/or sinuses and with their tumor tissue samples collected during the operation. Then, immunohistochemical staining procedures were taken to determine the expressive activities of PCNA, bcl-2 and p53 in the tissue samples respectively to explore their pathological implications in the developing process of malignant transformation from inverting papilloma. Results In the tissues of IP and IP with dysplasia, the positive expression rates of p53 were 0% and 40.0%, that of PCNA were 25.81% and 60.0%, and that of bcl-2 were 29.03% and 70.0% respectively, with very significant differences in these indicators between these two kinds of tissue samples. However, there were no such statistical differences present in these same indexes between the two kinds of tissues of IP with dysplasia and ones with malignant transformation to SCC. Conclusions The combined pathological effects of abnormally elevated activity of proliferation and declined activity of apoptosis may be the possible mechanisms underlying the malignant transformation from IP in nasal cavity and/or sinuses.
Malignant Transformation
Inverted Papilloma
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OBJECTIVE To investigate the expression of proliferating cell nuclear antigen (PCNA)and p27 protein in inverted papilloma (NIP)of nasal cavities and papransal sinuses and to explore the mechanism of the formation and growth of NIP from cell proliferating condition and cell cycle regulation. METHODS PCNA, p27 protein were stained with immunohistochemical technique (LSAB). There were four groups of patients47 cases in the NIP group, 49 cases in the nasal polyps group, 43 cases in the nasal squamous carcinoma group and 11 cases in the normal control group. RESULTS The PCNA labeling index(LI)in NIP group was significantly higher than that in the groups of nasal polyps and normal nasal mucosa (P0.001). But no significant difference was found between NIP and nasal squamous carcinoma group(P0.05). At the same time, there was no difference between primary NIP and recurrent NIP group (P0.05). p27 protein was expressed in all groups, but no difference was found among them (P0.05). CONCLUSION The PCNA labeling index may reflect the proliferating condition of NIP, but does not have relationship with NIP recurrence. And the role of p27 in the development of NIP needs more investigation.
NIP
Inverted Papilloma
Nasal Polyps
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In order to investigate the changes in cellular distribution of the glycocalyces in nasal inverted papilloma, formalin-fixed, paraffin-embedded biopsy specimens of inverted papilloma were analyzed by the avidin-biotin-peroxidase complex technique for the demonstration of peanut agglutinin (PNA) receptors, concanavalin A (Canavalia ensiformis agglutinin; ConA) receptors, carcinoembryonic antigen (CEA), and keratin, and compared with normal nasal mucosa, nasal polyps, and papillary adenocarcinoma. The inverted papillomas were positive for PNA and CEA, to the same degree as papillary adenocarcinoma. Their PNA binding was related to the degree of dysplasia. The ConA reaction was intermediate between that of normal mucosa and adenocarcinoma. The results suggest that the alteration of cellular glycoprotein structure in inverted papilloma is associated with its biologic characterization.
Glycoconjugate
Inverted Papilloma
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The aim of our study was an evaluation of the expression of cell proliferation markers (PCNA and Ki-67) in conjunctival and eyelid papillomas and squamous and basal cell cancers. A series of 9 cases of squamous cell cancer (SCC), 15 cases of basal cell cancer (BCC) and 43 cases of squamous cell papilloma (SCP) were assessed using the immunohistochemical method with monoclonal antibodies. PCNA overexpression was observed in 100% of SCP, in 88.8% of SCC and in 100% of BCC cases. Ki-67 overexpression was seen in 32.5% of cases of SCP, in 22.2% of SCC and in 66.6% of BCC. The results showed that an evaluation of Ki-67 expression is the most valuable cell proliferation marker.
Proliferation Marker
Ki-67
Basal (medicine)
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Twenty‐four oral papillomas were fixed in a 10% formalin solution, and 5 μ paraffin sections were prepared by normal procedure. Using monoclonal antibody NCC‐RAS‐001 as the primary antibody, the expression of ras p21 in the oral papilloma was searched immunohistochemically by the Histostain SP kit. The ras p21 was detected in 10 (41.7%) of 24 oral papillomas. In the histologic expression, it was less frequently detected in keratinized cell layer and basal cell layer, and it was most often detected in the cytoplasm of the cells in the spinous cell layer. These findings suggested the close relation between the expression of ras p21 and the epithelial proliferation and differentiation in the oral papilloma.
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Aims-To clarify p21 waf1/cip1 expression in sinonasal lesions.Methods-Archived surgical specimens from 38 patients were investigated by means of immunohistochemistry. p21 waf1/cip1 staining was evaluated in the diVerent layers of the epithelium.In addition, human papillomavirus (HPV) infection and p53 protein overexpression were assessed and correlated with p21 waf1/cip1 expression.Results-p21 waf1/cip1 staining was negative in non-papillomatous nasal mucosa.HPV infection and p53 protein overexpression were not seen.Sixteen of 20 inverted papillomas showed p21 waf1/cip1 expression.HPV infection was found in 16 cases and p53 protein overexpression was present in 13 specimens.Expression of p21 waf1/cip1 was restricted to surface cells in five cases, but involved basal/parabasal cells in 11 specimens.Immunoreactivity for p21 waf1/cip1 in basal/parabasal cells colocalised with p53 protein overexpression.Enhanced expression rates for p21 waf1/cip1 were seen in transitional and squamous epithelium compared with columnar epithelium.p21 waf1/cip1 expression involved only surface cells in cylindrical cell papillomas.HPV infection and p53 protein overexpression were detected in all specimens.One of five squamous cell carcinomas showed p21 waf1/cip1 expression.HPV infection was seen in two cases, and all carcinomas showed p53 protein overexpression.Conclusions-Expression of p21 waf1/cip1 is associated with terminal diVerentiation in surface cells in inverted papillomas and cylindrical cell papillomas, but not in non-papillomatous nasal mucosa.Overexpression of p53 protein colocalises with p21 waf1/cip1 expression in basal/parabasal cells in inverted papillomas but not in cylindrical cell papillomas.Expression of p21 waf1/cip1 in squamous cell carcinomas involves a subset of tumours with p53 protein overexpression.
Basal (medicine)
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Caveolin 1
Nasal Polyps
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The recurrent sinonasal inverted papilloma (IP) could be transformed to sinonasal squamous cell carcinoma. We use protein expression patterns by immunohistochemical method to see whether the expression of p53, p16, p21, and p27 belongs to cell-cycle-regulators and PCNA (proliferating cell nuclear antigen) and Ki-67 the proliferation markers in sixty patients with sinonasal inverted papilloma, and 10 of them with squamous cell carcinoma transformation. Significantly elevated levels of Ki-67, p27, and PCNA in IP with squamous cell carcinoma transformation of sinonasal tract compared with inverted papilloma were revealed. No variation of p16, p21, PLUNC (palate, lung, and nasal epithelium clone protein) and p53 expression was correlated to sinonasal IP malignant transformation by multivariate survey. However, we found elevated PLUNC expression in IPs with multiple recurrences. Finally, we found that PCNA, p27 may interact with CDK1 which promote IP cell proliferation and correlate to sinonasal squamous cell carcinoma. Ki-67 could work throughout the cell cycles to cause malignant transformation. In conclusion, this is a first study showing the correlation of Ki-67, PCNA interacted with CDK1 might lead to malignant transformation. Elevated PLUNC expression in the sinonasal IPs was related to multiple recurrences in human.
Inverted Papilloma
Malignant Transformation
clone (Java method)
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