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    Staining bacterial spores for observation under microscopy
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    Keywords:
    Stain
    Bacterial spore
    Negative stain
    Differential staining
    Gram staining
    SUMMARY We have developed a new staining procedure that combines the traditional Gram staining for bacteria and the Weber's chromotrope staining method, the standard technique for the detection of microsporidia spores in clinical Specimens. This “Gram‐chromotrope” staining technique enhances the staining characteristics of microsporidia spores and facilitates the easy detection and differentiation of spores from other microorganisms that are found in clinical specimens, especially stool samples. This new technique is fast, reliable, and simple to perform, and can be easily adapted for use in clinical laboratories.
    Gram staining
    Gram