Study on the Electrochemical Activity of Lactoperoxidase and Concanavalin A Co-modified Electrode

Journal of Instrumental Analysis (2010)

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Abstract:

Direct electrochemical behavior of lactoperoxidase(LPO) was firstly obtained by employing a concanavalin A(Con A) and lactoperoxidase co-modified gold electrode.Then the electrocatalytic activity of LPO toward hydrogen peroxide(H2O2) and the influence of nitric oxide(NO) on the electrocatalytic activity of LPO were investigated.With the aid of Con A,LPO displayed a pair of quasi-reversible redox peaks in pH 7.4 phosphate buffer solution with a formal redox potential of-190 mV in cyclic voltammogram(CV) and showed typical thin-layer electrochemical behavior.The co-modified electrode exhibited electrocatalytic activity toward the reduction of H2O2,which was utilized to fabricate a H2O2 biosensor with the detection range of 2.0×10-5-4.0×10-3 mol/L.The experimental result also proved that NO showed a suppressive effect on the reduction of H2O2 at the concentration of micro-molar level.

Keywords:

Lactoperoxidase

Topics:

Electrochemical sensors and biosensors

Advanced Nanomaterials in Catalysis

Electrochemical Analysis and Applications

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