ETIOLOGY DETECTION OF VAGINAL SECRETION IN 5446 PATIENTS WITH VAGINITIS AND ITS CLINICAL SIGNIFICANCE
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Objective To investigate the pathogens in vaginal secretion of 5 446 patients with vaginitis in this region.Methods Sampls of vaginal secretion were collected from patients with vaginitis.Trichomonas and Candida mycoderma were detected by normal saline smear method,and bacterial vaginosis by sialidase technique.Results Of 5 446 samples,the positive was detected in 2 466,the overall detection rate being 45.2%.In which,trichomonas was detected in 123 cases(2.3%),candida mycoderma in 1 417(26.0%),bacterial vaginosis in 1 466(26.9%).Conclusion Simultaneous detection of trichomonas,candida mycoderma and bacterial vaginosis in vaginal secretion can better reflect the pathogen in patients with vaginitis,so as to help clinicians make a correct diagnosis and treatment.Keywords:
Trichomonas
Vaginal secretion
Trichomonas Vaginitis
Vaginal disease
Etiology
Leukorrhea
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A test included 40 women in the reproductive age with clinical symptoms of vaginitis and microbiological examination. They were treated by combined therapy of vaginal tablets of nifuratel, 500 mg and nistatin 200 000 i. u. during six days, after which they underwent gynaecological reexamination and repeated microbiological examination of vaginal and cervical smears. An analiysis of vaginal secretion found bacterial flora in 34 smears (65%), fungus (Candida albicans) in 15 (24%) and Trichomonas vaginalis in 7 (11%). Local vaginal therapy in vaginitis caused by Trichomonas vaginalis was successfull in all 7 patients, vaginitis caused by Candida albicans was successly treated in 14 (93%) patients. Bacterial vaginitis was cured in 29 (71%) patients during this tharapy. Local vaginal combined therapy of nifuratel and nistatin is eficient in patients with vaginitis caused by fungi and Trichomonas vaginalis too.
Trichomonas Vaginitis
Trichomonas
Vaginal flora
Vaginal disease
Leukorrhea
Trichomoniasis
Vaginal discharge
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[Objective] To investigate the pathogens in vaginal secretion of 260 patients with vaginitis in our region.[Method] Samples of vaginal secretion were collected from patients with vaginitis.[Result] 216 patients were positive cases and the overall detection rate was 83.1%.Bacterial vaginosis,fungal vaginitis,trichomonas vaginitis and aginitis of mixed infection were 96(36.9%),58(22.3%),38(14.6%) and 24(9.2%) cases,respectively.[Conclusion] The types of vaginitis in our district were mainly bacterial vaginitis and fungal vaginitis Foreign population were mainly infected by trichomonad and the local women were mainly mixed infection by trichomonad and fungi.
Trichomonas Vaginitis
Vaginal secretion
Trichomoniasis
Trichomonas
Vaginal disease
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Objective Tostudytheprevalenceofvaginitisin Chaozhouand theapplication ofenzymaticsialicacid assayin bacterialvagi-nosis(BV).Methods Vaginal secretions were collected from 2018 vaginitis outpatients,Trichomonas vaginalis,fungus and cleaness were detec-ted with normal saline smear method,enzymatic determination of sialic acid was carried for the rapid detection of BV.Meanwhile,100 BV samples were randomly selected and tested with traditional method.Results Of 2018 cases of vaginitis,BV were found in 303 cases(15.0%),fungal vaginitis in 210 cases(10.4%),trichomonas vaginitis in 50 cases(2.5%),BV combined with fungal vaginitis in 35 cases(1.7%),and BV combined with trichomonas vaginitis in 4 cases(0.2%).There was no statistically significant difference between traditional method and enzymatic sialic acid assay for the detection of BV(P 0.05).Conclusion In Chaozhou the prevalence of BV is higher than that of fungal vaginitis and trichomonas vaginitis,suggesting that it is necessary to routinely check BV to provide timely and accurate diagnosis and treatment for vaginitis.Compared with the traditional one,enzymatic sialic acid assay is more rapid and effective for the detection of BV.
Trichomonas Vaginitis
Trichomonas
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While vaginitis caused by Trichomonas vaginalis is now less frequent, fungal Candida spp. infections are frequently found and the bacterial vaginosis is one of the most common vaginal diseases caused by anaerobic microorganisms such as Gardnerella vaginalis. Purpose of this study is to evaluate the usefulness of a rapid molecular test for the diagnosis of vaginitis/bacterial vaginosis in symptomatic women. In our clinic, between January 2008 - June 2009, we admitted 1592 (388 were pregnant) symptomatic women with a specific request to test them for fungi, Trichomonas and Gardnerella on vaginal fluid. The samples were tested with the kit Affirm (Becton Dickinson) that provides results in 40 minutes and allows the simultaneous identification of the DNA of Gardnerella vaginalis, Candida spp. and Trichomonas vaginalis. One hundred and eight out of 388 pregnant women were positive only for Gardnerella; 53 for Candida and Gardnerella; 59 were positive only for Candida and 10 for Trichomonas. As to the remaining 1204 not pregnant patients, 356 were positive only for Gardnerella; 98 for Candida and Gardnerella, 143 were positive only for Candida and 21 for Trichomonas.A simultaneous positivity for Trichomonas and Candida or for Trichomonas and Gardnerella has not been observed in any case. Molecular testing is obviously more sensitive and specific than culture method and microscopic research, especially for the detection of Gardnerella. It also enables differential diagnosis between bacterial vaginosis and vaginitis and therefore allows a targeted therapeutic intervention.
Gardnerella vaginalis
Trichomonas
Vaginal disease
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Vaginal infections lie among the most common causes women ask for medical advice. In order of frequency bacterial vaginosis, vulvovaginal candidiasis, and trichomoniasis are responsible for 90% of vaginitis/vaginosis.To evaluate a DNA hybridization test for simultaneous molecular detection of Gardnerella vaginalis, Candida species and Trichomonas vaginalis, as an alternative to conventional microbiological methods.Cohort, cross-sectional, and comparative study of 1,003 vaginal samples from symptomatic women from our health-care area. Two swabs were obtained from each woman, one for routine microbiological diagnosis of vaginal infection (wet mount, Gram stain, and mycological culture) and the other for the DNA hybridization test (Affirm VPIII, Becton Dickinson). This method detects clinically significant levels of G. vaginalis (2 x 10(5) CFU/ml), Candida spp. (1 x 10(4) cells) and T. vaginalis (5x103 trichomonads).Out of the 1,003 women studied, 30.6% tested positive for bacterial vaginosis, 23.3% for vulvovaginal candidiasis, and 0.5% for trichomoniasis. The Affirm VPIII method turned out positive in 27.5%, 27.4% and 0.5% of cases, respectively. No statistically significant differences were found between the molecular technique and conventional methods for microbiological diagnosis of vaginitis/ vaginosis (p < 0.05).The Affirm VPIII test correlated well with wet mount, Gram stain and mycological culture. Although its cost is relatively high, it is fast, reproducible, easy, and can be done in either clinical laboratories or Gynecology offices, which permits prescribing a specific early treatment.
Trichomoniasis
Gardnerella vaginalis
Gram staining
Trichomonas Vaginitis
Trichomonas
Vaginal disease
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Objective To understand vaginal pathogenic infections.Methods Cleanliness indexes,fungus,trichomonad and bacterial vaginosis(BV)were detected from vaginal secretion samples.Results Group of 447 cases of vaginal discharge physical examination,normal cleanliness(Ⅰ°～Ⅱ°) 255 cases,cleanliness Ⅲ°172 cases,Ⅳ°20 cases.Non-specific vaginitis was detected in 43.17%.Outpatients were 447 cases,cleanliness normal(Ⅰ°～Ⅱ°) 71 cases,cleanliness Ⅲ°308 cases,Ⅳ°68 cases of non-specific vaginitis was detected in 84.50%.Medical group of people detected 28 cases of fungal vaginitis,the detection rate of 5.85%;outpatients were detected in 114 cases,the detection rate of 25.63%;trichomonas vaginitis medical group population were not detected,detection of outpatients 8 cases,the detection rate of 1.81%.G-diplococcus medical groups were not detected,outpatients were detected in 2 cases,the detection rate of 0.42%.Conclusion The incidence of BV is high in this region,it should make the appropilate prevention and treatment efforts.
Vaginal secretion
Vaginal discharge
Trichomonas
Trichomonas Vaginitis
Leukorrhea
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Objective To investigate diagnostic value of BV detection technology in diagnosis of vaginal infection.Method 6 500 cases form Sep.2009 to Feb.2010 had been tested not only by united three detections but also by wet plate microscopy of physiological saline on detecting mycetes,infusorium and the cleaness.Result There were 1 274 cases of colpomycosis,with 19.6% positive rate,195 cases of trichomonas vaginitis,with 3.0% positive rate.There were 1 612 cases of BV with 24.8% positive rate by united three detection technology.There were 465 cases infection of colpomycosis with 4.9% positive rate and 79 cases infection of trichomonas vaginitis with 4.9% positive rate in those BV.Conclusion Three detections are easy operation,high speed and accuracy.It can help diagnose bacterial vaginosis with reflecting the function of normal flora and pathogenic bacteria in vagina as leukorrhea rouitne determination.
Trichomonas
Leukorrhea
Vaginal flora
Trichomonas Vaginitis
Infection rate
Vaginal infections
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Objective To understand the status of pathogen infection of the vaginitis and vaginal secretion of the famale patients in the local area.Methods Collect the vaginal secrtion samples from the patients with vaginities in the Gynecologic Outpatient Department,and detect the trichomonas and Candida mycoderma bacteria by using normal saline smear method and have a rapid detection of Bacterial Vaginosis by using the method of sialidase.Results In 2 084 cases,38 cases of trichomonas were detected,with the positive rate being 1.8%;510 cases Candida mycoderma bacteria were detected,with the positive rate being 24.5%;Alltogather,531 cases with Bacterial Vaginosis positive were detected out and the positive rate was 25.5%.Conclusion Simultaneously,the trichomonas,Candida mycoderma bacteria and Bacterial Vaginosis of the vaginal secretion from the vaginitis patients were detected,which can beter reflect the status of pathogen infection of vaginal secretion from the patients.So it can be used as screening program of leukorrhea routine examination for the outpatients from Obstotrics and Gynaecology Department in the local area.
Trichomonas
Vaginal secretion
Leukorrhea
Outpatient clinic
Trichomonas Vaginitis
Vaginal discharge
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To determine the incidence and microbial aetiology of vaginitis, and the usefulness of Gram staining of vaginal fluid in detecting the aetiology of vaginitis in Sri Lankan women.Prospective study with microbiological investigation of high vaginal specimens from symptomatic and healthy groups of women.A gynaecological clinic of the De Soysa Hospital for Women, and a family planning clinic of the Family Health Bureau, Colombo.114 untreated patients with symptoms suggestive of vaginitis and 109 healthy controls.Candida species were isolated from 36% patients and 9% of controls. Gardnerella vaginalis was recovered from 8.8% of patients and 7.3% of controls. The bacterial vaginitis group consisted of several distinct subgroups, viz Gardnerella vaginalis vaginitis, anaerobic vaginitis, non-anaerobic vaginitis and polymicrobial vaginitis. The incidence of trichomoniasis was lower (4.4%) compared to other studies. Other bacteria (anaerobes, enterococci, coliforms, Group B Streptococci) were isolated in significantly higher numbers in the symptomatic group compared to the control group. Microscopy showed that the majority (73%) of the Candida vaginitis patients retained the normal Lactobacillus morphotype. In bacterial vaginitis Lactobacillus morphotype was replaced by other bacterial morphotypes.In our study 36% had candidiasis, 4.4% had trichomoniasis and 47% had bacterial vaginitis. In 12% there was no detectable pathogen. Microscopy of vaginal fluid is useful in determining the aetiology of vaginitis.
Gardnerella vaginalis
Trichomoniasis
Trichomonas Vaginitis
Etiology
Vaginal disease
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