A Simple Making Method of Tissue Microarray and Validation Study on Immunohistochemistry
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Objective To search a simple making mothd of tissue microarray and evaluate its validation. Methods A new, simple and easy method was designed to improve the technology of TMA during the experiment. Tissue microarrays with 1.6 mm in diameter tissue core were constructed from 124 cases of breast cancer. The tissue microarrays were stained for oestrogen receptor(ER),progesterone receptor(PR)and c-erBb2 using immunohistochemistry.At the same time the stains on the tissue microarrays were compared with that from the full tissue sections. Results A highly significant association was observed between the staining scores(0 ～ 7)obtained from the full tissue sections and from the tissue microarrays. Concordance for the receptor status(positive / negative)of the whole section and the tissue core were 94.35% for ER,93.55% for PR.and 91.94% for c-erBb2.The discordance between full section and tissue microarrays was studied using Two - Related - Samples tests and the result had no statistical significance(P0.05) . Conclusion Tissue microarays with 1.6 mm in diameter tissue core made by simple method can represent full tissue section on immunohistochemistry study.We believe our method of tissue microarray is a reliable and validKeywords:
Tissue microarray
Concordance
Oestrogen receptor
Breast tissue
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Objective To compare the measurements of nuclear stereological parameters of normal adult alveolar type Ⅱ epithelial cells,human embryonic lung cells and lung cancer cells in conventional paraffin-embedded tissue sections and microarrays a,and to explore the reliability of stereological quantification based on tissue microarray.Methods Surgical resection of lung cancer specimens: 52 cases of squamous cell carcinoma,38 cases of adenocarcinoma,10 cases of large cell carcinoma,4 cases of small cell carcinoma,20 cases of normal lung tissue and 20 cases of embryonic lung tissue.The TMA technology was used to construct a paraffin tissue microarray containing 760 tissue cores.Using Image-Pro image analysis system to test cells and their nuclear-related stereological parameters:Vvn,Svn,Rsv,Rnp and λn.The reference space of nucleus was according to the cancer cells and that of nuclei was according to the nucleus.Results The values of stereological parameters of the nuclei measured on paraffin tissue microarrays and corresponding routine histological sections were basically similar,with statistically non-significant differences between them,P0.05.Conclusion The results of this study demonstrate that stereological measurement of cell nuclei based on both tissue microarrays and the corresponding routine histological sections is reliable and basically similar,but the use of tissue microarray is feasible and more efficient.
Tissue microarray
Stereology
Large cell
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Tumor Neoangiogenesis by CD31 and CD105 Expression Evaluation in Breast Carcinoma Tissue Microarrays
Abstract Purpose: The aim of the study was to evaluate CD31 and CD105 immunohistochemical expressions in tissue microarrays from 360 breast carcinomas. Study design: Computerized (ACIS/Chromavision) assisted image analysis was performed to compare immunoreactions in tissue microarrays with those in current paraffin and frozen sections. We also aimed to determine the CD105 and CD31 prognostic significance and relevance in routine practice by correlating results of immunodetections with patients’ (n = 360) outcome (14.3-year follow-up). Results: The results show (a) that in tissue microarrays, the CD31 and CD105 expression quantified by image analysis device did not correlate with the measurements assessed on routine paraffin sections; (b) that CD105 expression is endowed of a prognostic significance in paraffin sections in terms of overall survival (P < 0.01), whereas in contrast, CD31 on paraffin sections did not correlate with patients overall survival; (c) that semiquantitative analysis of CD105 expression correlated with the image analysis measurements in frozen sections (ρ = 0.671, P < 0.01) and paraffin (ρ = 0.824, P < 0.01) sections. However, paraffin sections were less immunostained than frozen ones. Conclusions: It is concluded (a) that CD105 may be suitable in paraffin sections to evaluated neoangiogenesis; and (b) that tissue microarrays are not suitable substrates for neoangiogenesis evaluation as a prognostic indicator in breast carcinomas, in contrast to current tissue sections.
Tissue microarray
CD31
Clinical Significance
Breast carcinoma
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Objective Tissue microarrays allow high throughput molecular profiling of cancer specimens by immunohistochemistry. This study is aimed at investigating the reliability and validity of tissue microarrays for immunophenotyping.Methods We constructed triplicate tissue microarrays (TMAs) containing specimens from 418 patients with non small cell lung cancer (NSCLC), and randomized selected 50 full tissue sections from these tumors. TMAs and full tissue section slides were immunohistochemically stained with antibodies against Ki 67 and p53. Data on full tissue sections were compared to the results of TMAs.Results Concordance for Ki 67 and p53 staining between tissue arrays with triplicate cores per tumor and full sections were 98% and 96%, respectively. TMAs (three cores per tumor) were reliable for detecting of Ki 67 and p53 in NSCLC tissues, Kappa value were 0.95 and 0.91 , respectively.Conclusion Triplicate 0.6 -mm core biopsies sampled on tissue arrays provide a reliable system for high throughput expression profiling by immunohistochemistry when compared to standard full sections, and suit for large scale retrospective clinical study.
Tissue microarray
Concordance
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Malignant fibrous histiocytoma (MFH) represents a heterogeneous soft tissue sarcoma entity. The authors compared different methods to determine immunohistochemical staining in whole tissue sections, evaluated the tissue microarray technique, and assessed immunohistochemical heterogeneity using the proliferation marker Ki-67 in 47 histopathologic tumor blocks from 11 MFHs. Whole tissue sections were assessed counting 400 cells along a line and counting all cells in 10 high-power fields (0.16 mm 2 ) with mean Ki-67 expression levels of 13% and 11%, respectively. For the tissue microarray technique, two to three 0.6-mm diameter biopsies were studied from each of the 47 tumor blocks. Good correlation was obtained between whole tissue immunohistochemistry and tissue microarray with the microarray method, giving on average 8.6% greater Ki-67 expression levels than the reference method. Immunohistochemical tumor heterogeneity, evaluated using the high-power field method, showed a median standard deviation of 2.3% within the tumor blocks and 2.5% between the blocks from the same tumor. The authors concluded that the tissue microarray technique yields good quality staining and expression levels for Ki-67 comparable with whole tissue methods in MFH, but because of tumor heterogeneity, several tumor blocks ideally should be studied and, because of loss of material in the microarray process, multiple biopsies should be taken. The feasibility of tissue microarray for immunohistochemical studies of soft tissue sarcomas offers new possibilities to study multiple markers in large tumor materials.
Tissue microarray
Ki-67
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Tissue microarray
Serous carcinoma
Immunostaining
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Tissue microarray
Negative control
Positive control
Stain
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Objective To examine the reliability of tissue microarrary(TMA) for detecting tumor marker in soft tissue sarcoma compared to the immunohistochemistry.Method Tissue microarray technology was used to detect the expression of Ki-67,PCNA and P53 in 80 cases of STS and compared the results with the immunohistochemistry.Results No difference was found between the immunohistochemistry results by 1.5 mm tissue microarray and the negligible difference was found between by traditional pathological technology and by tissue microarray in soft tissue sarcoma.Conclusion On the positive rate of Ki-67,PCNA and p53,no significant difference is found between the results with the traditional pathological investigation and with the tissue microarray(P0.05).It has important practical significance and broad application prospect in pathology.
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We described earlier the possible role of ATPaseC1 expression as a diagnostic and prognostic marker for oral cancer; others have reported its use for tumors of the lung and breast. We assessed ATPaseC1 expression in a sample of oral squamous cell carcinoma (OSCC) using tissue microarrays (TMAs) to analyze the relation between ATPaseC1 expression and clinical, histopathological and prognostic parameters. We performed a retrospective study of 48 cases of OSCC. We constructed TMAs using two different regions of each tumor. V-ATPaseC1 immunohistochemistry was performed and assessed semiquantitatively. ATPaseC1 staining was observed in most of the neoplastic cells in all tumors. Staining was diffusely cytoplasmic and, to a lesser extent, nuclear. The degree of concordance between the measurements performed in tissue microarray 1 (TMA1) and tissue microarray 2 (TMA2), as evaluated using the intra-class correlation coefficient (ICC), was low. We found great variability in the immunohistochemical staining of the different regions of each tumor. We found 16 cases with mild expression (33.3%), 20 with moderate expression (41.7%) and 12 with intense expression (25%). Differences in the clinical-pathological variables studied were not statistically significant. The difficulty of immunohistochemical evaluation, the heterogeneity of the carcinomas and the fact that evaluation of expression requires semiquantitative analysis render the reliability of the results obtained from TMA-based techniques questionable.
Tissue microarray
Concordance
Anatomical pathology
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The accuracy of immunohistochemical (IHC) analysis on tissue microarray (TMA)-based studies largely depends on the uniformity of the staining pattern for a given antibody and minimal intratumor heterogeneity of a given tumor. Our study was designed to investigate the concordance of expression in TMA and whole sections of estrogen receptor (ER), progesterone receptor (PR) and HER2 using IHC analysis for ductal carcinoma in situ (DCIS) of the breast. Seventy-five consecutive cases of DCIS were retrieved, reviewed and used to construct the TMA. IHC analysis of the expression of ER, PR, and HER2 were performed on TMA and whole sections of the corresponding cases, and the results were compared. The specificity and sensitivity for TMA-based assays were 87.0, 75.9, 90.6 and 90.4%, and 76.1, 27.3 for ER, PR and HER2, respectively. The concordance and discordance were 89.3, 76.0 and 72.0%, and 6.7, 13.3 and 16.0% for ER, PR, HER2, respectively. The kappa values were 0.83, 0.89 and 0.42 for ER, PR and HER2, respectively. The non-concordance rates were inversely related to core number, with 46.67, 22.67 and 11.56% for one core, two cores, and three cores, respectively, per marker per case (p < 0.001), but not associated with tumor size. Our results showed that the intratumor heterogeneity and the number of cores have a great impact on the results of TMA-based studies. Increasing the number of tissue cores per case may help improve the accuracy and concordance with whole section results. Although TMA remains an effective tool for translational research, we should be cautious in our interpretation of these results.
Concordance
Tissue microarray
Progesterone receptor
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Objective:To explore the feasibility of application of tissue microarray(TMA) to placental immunohistochemistry.Methods:34 normal placenta and 60 placenta of puerpera with ICP were collected. The tissue microarry was made up with these placenta tissues and stained by histochemical method.Results:It was successfully made up a placental TMA paraffin block containing 7×15 square matrix. The tissues in this microarray were clear and the lost rate of the microarray was 3.12%. The observation results of TMA slice stained by HE staining and NF-κB p65 protein immunohistochemical staining under the microscope were the same as that of the common tissue slices.Conclusion:TMA is stable and simple and can be applied to placental immunohistochemistry. The examination results of TMA is of good comparability.
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