The screening and identification of oil degradation bacteria producing surfactant
0
Citation
0
Reference
20
Related Paper
Abstract:
7strains degrading oil were isolated from long-term oil-contaminated soil and they were numbered as bacteria 2-1,bacteria 7-1,bacteria 1-2,bacteria5-2,bacteria 7-2,bacteria oil 3,bacteria oil 5,of which bacteria 2-1and bacteria 7-1were identified as Serratia marcescens,bacteria 1-2as Raoultella planticola,bacteria 5-2,bacteria oil 3,and bacteria oil 5as Klebsiella genus,bacteria 7-2as Bacillus cereus by morphological observation,Biolog identification and 16SrDNA gene sequence analysis.Bacteria 1-2,bacteria 5-2and bacteria 7-2enabled the surface tension of the fermentation liquid reduce from 36.1mN/m to 20.2mN/m, 20.74mN/m and 21.78mN/m,indicating the surfactants produced by the bacteriums have a strong emulsification crude capacity and show great potential.Keywords:
Bacillus (shape)
Cite
Oil contamination in Iran due to high application of oil compounds, is one of the most dangerous pollution factors. Bioremediation is a prime strategy for remediation, by which the pollutants can be removed by making use of microorganism or any biological process that employes microorganisms or their enzymes to rehabilitate the environment altered by the contaminants. With regards to the importance of this method, screening and isolation of oil-degrading bacteria from contaminated site of Bushehr province were done in Carbon Free Minimal Medium enriched by gas oil. In this study 19 isolates of bacteria (PDB 1-19) were isolated, and the molecular and kit identifications showed different genus and species such as Stenotrophomonas maltophilia, Ralstonia sp., Vibrio sp., Sphingobacterium sp., Zymomonas sp., Pseudomonas aeruginosa, Paracoccus sp., Pantoea sp., Achromobacter xylosoxidans, Acinetobacter johnsonii, Serattia odorifera, Pseudomonas alcaligenes, Entrobacter cloacae, Pseudomonas stutzeri and Chryseobacterium sp.. In order to determine the efficiency of these bacteria in hydrocarbon- degradation an inoculum of pure culture of bacteria containing 10 8 cfu/ml was used in liquid assay, which was performed in factorial experiment based on completely randomized design with 2 replications. Utilization of hydrocarbon sources was again detected in CFMM broth supplemented with 2% gas oil where the growth turbidity (OD600nm) as an indicator for utilization of hydrocarbon and bacterial growth was measured at different times of 0, 48, 168, 216 and 312hr. Results showed that in CFMM broth assay all main factors including bacteria and time as well as their interaction were significant (p<0.01). These bacteria were effectively able to mineralize gas oil and among bacteria, best results was achieved by Chryseobacterium sp.. The ability of bacterial isolates for degradation of oil-compounds increased by increasing time of incubation.
Stenotrophomonas
Chryseobacterium
Microbacterium
Achromobacter
Stenotrophomonas maltophilia
Alcaligenes
Pseudomonas stutzeri
Cite
Citations (1)
Halomonas
Marine bacteriophage
Comamonas
Isolation
Cite
Citations (48)
Lipases have many applications in biotechnology, thanks to their ability of acylglycerides hydrolysis. They alsp possess the unique feature of acting at the lipid-water interface, which distinguishes them from esterases. Commercially useful lipases are produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The greatest production of lipase takes place under optimum conditions such as appropriate temperature, suitable carbon, nitrogen sources, etc. This study tries to collect lipase-producing bacteria from the soil of oil-extraction factories and identify isolated bacteria, while creating optimum conditions for lipase production by bacteria.
Having collected three soil samples from an oil extraction factory, lipase-producing bacteria have been identified, based on biochemical and morphological tests. Finally the optimal conditions for lipase production as well as molecular analysis has been evaluated.
During the study, among the different bacteria, the strain to produce highest lipase has been selected. It has been found out that the optimal conditions for lipase production by this strain is as follows: 48 hours of incubation; incubation temperature of 37 °C; pH of 7; agitation speed of 150 rpm; peptone extract as the nitrogen source; and olive oil as a carbon source.
A lipase-producing bacterium has been identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis, identified as Pseudomonas spp.
Cite
Citations (9)
A study was undertaken to investigate the distribution of biosurfactant producing, oil degrading bacteria in the oil-contaminated environment. Among 32 oildegrading bacterial isolates 80% exhibited biosurfactant production. They belonged to the genera A4icrococcus, Bacillus, Cotynebacterium, Flavobacterium, Pseudomonas, Acinetobacter, Moroxella and jlavobacterium, They emulsified xylene, benzene, hexane, crude oil, kerosene, gasoline, diesel and olive oil for various degrees. The first five of the above had highest emulsification activity and crude oil degradation ability and therefore were selected for the preparation of mixed consortium for bioremediation studies, Close inspection of the most efficient culture Pseudomonas sp, DS10-129 showed a maximum of 7.5 gl-l rhamnolipid type biosurfactant which resulted in surface tension reduction in the culture broth from 68mN m“’ to 29mN m-l at 84 h incubation. Mass spectrometry examination revealed the presence of both mono and dirhamnolipids (both Rha-Clo-CIOand Rha-Rha-Clo-C1o),The biosurfactant producing isolates had a broad spectrum of emulsification activity with a range of hydrocarbon compounds. The potential of their use in oil bioremediation is discussed.
Rhamnolipid
Kerosene
Cite
Citations (7)
Petroleum is a mixture of hydrocarbon complexes with organic compounds from sulfur, oxygen, nitrogen and metal-containing compounds. These organic compounds can be used as substrate for bacterial growth. This study aimed to isolate and identify hydrocarbon degrading bacteria and phosphate solubilizing bacteria in oil-contaminated soil in Bojonegoro. This study used an exploration method to find each of the two types of hydrocarbon degrading bacteria and phosphate solubilizing bacteria from soil samples in Bojonegoro that contaminated by oil. Identification of isolates bacterial included macroscopic observations of bacteria, gram staining on bacterial cells and physiological tests. Macroscopic observations include the form of colonies, colony diameter, colony color, colony edge, and elevation. The physiological test using Microbact Identification System to determine the physiological characteristics of bacteria so that genera and types of bacteria can be known. The identification of organisms was based on changes in pH and use of the substrate. The results of data analysis were obtained from five types of bacteria from soil samples that contaminated by oil which were successfully isolated. After identification of species was done, four species of bacteria were obtained, namely Pseudomonas pseudomallei, Pseudomonas fluorescens-25, Flavobacterium odoratum, and Enterococcus sp.
Pseudomonas fluorescens
Cite
Citations (10)
Release of petroleum oil and its products into the environment is a worldwide concern. The present study focused on isolation, molecular identification, morphological and biochemical characterization of bacteria possessing hydrocarbon-degrading properties. The study also aimed at optimizing appropriate culture conditions for the isolates as well as screening for alkane hydroxylase enzyme. Out of twenty one microbes isolated, nine were selected based on their ability to utilize different hydrocarbons. The isolates were observed to mineralize heating oil, hexane, octane, toluene and diesel oil. PCR amplification of 16S rDNA gene revealed that the isolates belong to six different genera; Pseudomonas, Acinetobacter, Klebsiella, Enterobacter, Salmonella and Ochrobactrum. Based on their ability to degrade diesel oil, three isolates were selected and their growth conditions optimized. Optimum degradation was recorded at less than 1% substrate concentration, pH 7 and temperature range of between 30°C and 37°C and using yeast extract as nitrogen source. GC-MS analyses confirmed their diesel oil degrading properties. Alkane hydroxylase gene from one of the isolates (isolate 1C) was successfully amplified indicating its catabolic capabilities in degrading alkanes. Overall, the characterized isolates may constitute potential candidates for biotechnological application in environmental cleanup of petroleum contaminants.
Enrichment culture
Cite
Citations (15)
Objectives:The objective was according to the character of bacterium selecting a bacterium which could enhanced the proportion of the third MEOR.Methods:The methods was using the selective culture medium to select the bacterium,using the methods of identification to identify there character.Results:[WTBZ]two bacteria were isolated with the methods of enrichment culture and plate cultivation from oil of Dqing Oil Field.The belonged to Pseudomonas,the belonged to Bacillus.By identified,both of two bacterium could produced some materials such as carboxylic acid,mix gas and biosurfactants,and the No.1 the degradation ratio of crude oil was 65.2%,the No.2' the degradation ratio of crude oil was 62.1%.Conclusion:Two bacteria were isolated from oil of Daqing Oil Field could degrade the stick of crude oil effectively.
Bacillus (shape)
Cite
Citations (0)
Some bacteria present a capability in bioremediation of oil derivatives. In this study the bacteria existing in the plant rhizospere of polluted soils were isolated and purified. These bacteria were then exposed to a combination of commonly used petroleum products with different concentration. The bacteria that capable of biodegrading these compounds, were selected and determined using taxonomical keys and biochemical tests. Their efficiencies in removing the petroleum derivatives were compared. Finally, bacteria with the highest capability for degradation of all oil products were introduced. According to our results, Alcaligenese utrupha, Pseudomonas alcaligenes and Pseudomonas aeroginosa were isolated from rhizosphere of the soils contaminated by kerosene and gasoil; Pseudomonas alcaligenes, Bacillus coagulans, and Bacillus circulans were isolated from soils contaminated by kerosene and engine oil; and Pseudomonas alcaligenes was isolated from soils contaminated by gasoil and engine oil. Pseudomonas alcaligenes was able to biodegrade the all types of the studied oil products. The bioremediation efficiencies of this bactrium were 72, 63 and 56% repectively.
Alcaligenes
Kerosene
Bacillus circulans
Bacillus (shape)
Cite
Citations (0)
Oxidizing agent
Degradation
Enrichment culture
Cite
Citations (17)
Petroleum hydrocarbons are species of dangerous contaminants in nature. Scientists are looking for many years to find solution for removing contaminants from the soil and water environments. Today, the use of microorganism, for removing crude oil pollution from contaminated sites (bioremediation) is considered by scientists. The purpose of this research is to isolate and identify hydrocarbons degrading bacteria from Shiraz Refinery soil. In this study, nutrient agar (NA) and mineral salt agar (MM2) were used as basic cultures. Using the Techniques Replica plating, we transferred colonies on NA surface to the MM2 agar. For spraying hydrocarbons on the MM2 agar surface, we used spray plating technique. Finally we have identified different bacterial genera by using biochemical tests and morphological study. Upon examination on morphological studies and biochemical tests, it is determined that these strains belong to bacterial genera as follows: Bacillus, Corynebacterium, Staphylococcus, Streptococcus, Klebsiella, Escherichia, Acinetobacter, Alcaligenes, Shigella. Enterobacter. It is also discovered that these bacteria can break down Biphenyl, Naphthalene, Camphor and Phenenthrene. The results showed that Biphenyl, Naphthalene, Camphor and Phenanthrene were decomposed by 22, 23, 34 and 23%, respectively. Bacillus was 69% of the bacterial population and accounted as the most dominant bacterial genus. Statistical analysis showed that there is a significant relationship with the level of 0.05 among the station, the numbers, and the diversity of Gram-positive bacteria. Key words: Degrading bacteria, camphor, bioremediation, Bacillus, Shiraz Refinery.
Nutrient agar
Corynebacterium
Arthrobacter
Alcaligenes
Microbacterium
Camphor
Bacillus (shape)
Cite
Citations (28)