Butanol production from xylose fermentation in cellulose hydrolysate
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A strain was obtained by screening with which butanol can be fermented by using cellulose hydrolysate. The microorganism not only could use glucose of cellulose hydrolysate,but also could use xylose of cellulose hydrolysate. The growth characteristics,carbon source,nitrogen source and CaCO3 addition in batch fermentation were studied. High concentration glucose and xylose could not be consumed. The optimal concentration of xylose was 20 g/L in batch fermentation. So the initial concentration of xylose in cellulose hydrolysate was 20 g/L with 15 L fermentation reactor,and then cultured for 84 hours at 37 ℃. The yield of butanol was 10.95 g/L,yield of total solvent 16.78 g/L(acetone,ethanol,and butanol),utilization rate of xylose was 70% and total solvent conversion was39.4%. The economic loss by failure of utilization of xylose in cellulose hydrolysate fermentation was resolved.Cite
Enzymatic hydrolysate of sulfite bagasse pulp was used as the substrate of the fermentation to product fuel ethanol.In order to achieve the highest sugar utilization ratio,the best initial concentration of reducing sugars were optimized as follows: glucose 140 g/L,xylose 60 g/L,and the total sugar of enzymatic hydrolysate 80 g/L.In the subsequent fermentation,the enzymatic hydrolysate containing glucose 55.06 g/L,xylose 11.18 g/L and cellobiose 4.51 g/L was used as the substrate,22.98 g/L ethanol and 67.23% ethanol yield were obtained after 18 h fermentation.The glucose utilization rate and xylose utilization rate were 99.27% and 32.96%,respectively.C.shehatae could be used as appropriate strain by bagasse pulp into ethanol.
Bagasse
Enzymatic Hydrolysis
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Nanofiltration
Ethanol Fermentation
Ultrafiltration (renal)
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Bagasse
Ethanol Fermentation
Sweet sorghum
Bioconversion
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The effects of the concentration of sulfuric acid and the ratio of liquid to solid on xylose yield from sugar cane bagasse in its hemicellulose hydrolysis process were studied with the Quadratic Rotary Combination Design. Regression analysis showed that there was a marked regression relationship between the two factors and xylose yield. As the result of optimizing the hydrolysis conditions by regression equation, xylose yield of 24 g/100 g sugar cane bagasse was obtained when sulfuric acid concentration was 2.4 g/L and liquid to solid ratio was 6.2 under the conditions of stream pressure of 2.5 x 10(4) Pa and hydrolysis time of 2.5 h. The macroporous resin adsorption was proved to be a good method to reduce the concentration of yeast cell growth inhibitor in sugar cane bagasse hemicellulose hydrolysate and to enhance the hydrolysate fermentability. The hydrolysate treated with macroporous resin adsorption under pH2 was used as the substrate for xylitol production by a xylitol-producting yeast, Candida tropicalis AS2.1776. At an initial xylose concentration of 200 g/L, all xylose was consumed within 110 h with a xylitol production rate of 1.15 g/L.h, and a xylitol yield of 0.64 g/g xylose.
Bagasse
Hemicellulose
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The production of 2,3-butanediol was carried out from fermentation of xylose by Klebsiella oxytoca ZU-03,in which xylose was the main reducing sugar in hemicellulosic hydrolysate.Under optimized conditions: xylose mass concentration 90 g/L,corn liquor mass concentration 6 g/L,inoculum 10 %(volume fraction),initial pH value 5.5 and 30 ℃,concentration of 2,3-butanediol reached 36.22 g/L and yield of 2,3-butanediol from xylose was 0.45 g/g.
2,3-Butanediol
Klebsiella oxytoca
Butanediol
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Cellulosic ethanol
Pichia stipitis
Arabinose
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Enzymatic Hydrolysis
Lignocellulosic Biomass
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Xylose metabolism
Corn steep liquor
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Corn cobs hydrolysate produced by enzymatic hydrolysate pretreatment can be directly fermented using the adapted yeast strain at 30℃.The optimum fermentation conditions were as follows: inoculum rate 5%(volume ratio),inoculum age 20 h,nitrogen source 2.5 g/ L Bacto-yeast extract and 2.5 g/L Bacto-tryptone,volume charge 100 mL in 250 mL flasks,intitial pH value 6.Both of xylose utilization efficiency and xylitol yield were higher 65% respectively.This process can effectively reduce the pretreatment of costs,showing broad prospects of applications.
Yeast extract
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The steam-exploded hemicellulose hydrolysate of 100 g oil palm trunk chips was acid posthydrolysed under the condition of autoclaving at 120 °C with 1%w/v H2SO4 for 30 min. The highest amounts of xylose and glucose obtained were 8.15 g and 2.46 g, respectively. The optimal adsorption condition to remove toxic compounds in hemicellulosic hydrolysate was achieved in two steps. Five percents (w/v) of activated carbon were used in the first step for 30 min then 2% (w/v) activated carbon were used in the second step for 60 min. The comparisons were made between two sources of fermentation media, hydrolysate and pure xylose. It was found that fermentation of the hydrolysate with Candida magnolia TISTR 5664 gave xylitol at 0.08 g Yp/s of xylitol/g of xylose whereas fermentation of pure xylose gave xylitol at 0.29 g Yp/s of xylitol/g. The fermentation with C. guilliermondii FTI 20037 from the hydrolysate gave xylitol at 0.12 g Yp/s of xylitol/g of xylose whereas the fermentation from pure xylose gave xylitol at 0.30 g Yp/s of xylitol/g of xylose.
Hemicellulose
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