Protein Profiling of Brassica juncea (L.) Czern var. Ensabi at Different Developmental Stages
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In a soil bioassay, adult Deroceras reticulatum (Stylommatophora: Limacidae) and three different weight-classes of young Arion lusitanicus (Stylommatophora: Arionidae) were exposed to a single dosage (170 dauer larvae per g of soil) of the nematode Phasmarhabditis hermaphrodita monoxenically associated with the bacterium Moraxella osloensis. Groups of 10 slugs were continuously exposed to nematodes for 4 days, and then transferred individually to Petri-dishes containing a disc of Chinese cabbage as food. Food consumption—measured by image analysis—and slug mortality were recorded daily for 10 days. Food consumption was inhibited in both slug species tested. D. reticulatum stopped feeding 6 days after the start of nematode treatment, while all A. lusitanicus continued to feed. However, in the three weight-classes of A. lusitanicus (0.15 g, 0.24 g, 0.45 g), food consumption was reduced by at least 50 %. The greatest reduction in feeding, nearly 90 %, was noted in the smallest A. lusitanicus. The nematodes successfully killed D. reticulatum but were less efficient at killing young A. lusitanicus. At the end of the experiment, mortality was highest in D. reticultatum (98 %) and the smallest weight-class of A. lusitanicus (47 %). There was almost no mortality in the largest weight-class of A. lusitanicus treated with nematodes. P. hermaphrodita associated with M. osloensis can thus be considered as a biological control agent for young stages of A. lusitanicus for its effect as a feeding inhibitor, rather than for its ability to kill the slugs.
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The study used glyphosate resistant Brassica napus,planted in the field of Mianyang Normal College.The glyphosate resistant Brassica napus seeds wereplanted in the middle field,and the wild Brassica napus and Orychophragmus Violaceus were planted around the glyphosate resistant Brassica napus,in different directions in nature environment in 25 meters.The second year the wild Brassica napus and Orychophragmus Violaceus seeds were plantde in the field,and sprayed more glyphosate on the plants,and selected the plants which were not died.The leaves of not died wild Brassica napus were checked in the lab by PCR and Southern blot.The result indicated that four wild Brassica napus plant got the EPSPS gene from the glyphosate resistant Brassica napus,there are no gene flow between Orychophragmus Violaceus and glyphosate resistant Brassica napus.
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Different genotypes of Brassica Campestris were pot-cultured in paddy soil polluted by di-n-butyl phthalate(DBP), and the distribution of DBP in Brassica Campestris-soil system was studied adopting GC/MS. DBP accumulation of the leaves and roots of different genotypes of Brassica Campestris varied obviously. The contents of DBP in the leaves and roots of Teqing-60 Brassica Campestris and Youqingsijiu Brassica Campestris were all lower, Yashuquannianyoulu Brassica Campestris and Youqing-60 Brassica Campestris were all higher. The contents of DBP in the leaves existed definite positive correlation with the size of leaves areas. The distribution of DBP in different genotypes of Brassica Campestris-soil systems was different obviously. The contents of DBP in Xinxunsiwu Brassica Campestris in leaves and roots and in pot-cultured soil were lower; DBP contents in Teqing-60 Brassica Campestris and Xinxunsijiu Brassica Campestris in leaves and roots were lower, but DBP content in pot-cultured soil was higher; and DBP contents in Youqing-60 Brassica Campestris in leaves and roots were higher, and also higher in pot-cultured soil.
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In response to DNA damage, p53 undergoes post-translational modifications (including acetylation) that are critical for its transcriptional activity. However, the mechanism by which p53 acetylation is regulated is still unclear. Here, we describe an essential role for HLA-B-associated transcript 3 (Bat3)/Scythe in controlling the acetylation of p53 required for DNA damage responses. Depletion of Bat3 from human and mouse cells markedly impairs p53-mediated transactivation of its target genes Puma and p21 . Although DNA damage-induced phosphorylation, stabilization, and nuclear accumulation of p53 are not significantly affected by Bat3 depletion, p53 acetylation is almost completely abolished. Bat3 forms a complex with p300, and an increased amount of Bat3 enhances the recruitment of p53 to p300 and facilitates subsequent p53 acetylation. In contrast, Bat3-depleted cells show reduced p53–p300 complex formation and decreased p53 acetylation. Furthermore, consistent with our in vitro findings, thymocytes from Bat3-deficient mice exhibit reduced induction of puma and p21, and are resistant to DNA damage-induced apoptosis in vivo. Our data indicate that Bat3 is a novel and essential regulator of p53-mediated responses to genotoxic stress, and that Bat3 controls DNA damage-induced acetylation of p53.
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Pot experiment was conducted to study the enrichment rules of heavy metals Cd and Ni in different growth periods of Brassica juncea treated with EDTA.The results showed that with the growth of Brassica juncea treated with EDTA,the concentration of Cd and Ni was higher than the control group.It was indicated that the transfer effect could be improved by EDTA.After analyzing the 6 different growth periods from 28 d to 63 d,the total Cd content in 49 d in the shoot of Brassica juncea accounted for more than 90 % of the whole growth periods,and Ni content was above 80 %.Therefore,49 d was the appropriate time to harvest Brassica juncea.
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[Objective] The rules of uptake and activation of insoluble Pd in calcareous soil by Brassica juncea were studied.[Method] The pot experiment was carried out to study the effect of Brassica juncea on the absorption of insoluble Pb and the purification efficiency.[Result] Indian mustard could absorb insoluble Pb and grew well.Pb uptake by Brassica juncea was existed in root and higher than 80%.The difference DTPA-extractable Pb in rhizosphere and non-rhizosphere of Brassica juncea was not significant.The Pb purification rate of the soil by Brassica juncea was 0.01%-0.02% at this test condition.[Conclusion] The effect of Brassica juncea on the purification of insoluble Pb in simulated calcareous polluted soil was not ideal.
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Holcus lanatus
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HLA-B-associated transcript 3 (BAT3) was originally identified as one of the genes located within human major histocompatibility complex. It encodes a large proline-rich protein with unknown function. In this study, we found that a fragment of the BAT3 gene product interacts with a candidate tumor suppressor, DAN, in the yeast-based two-hybrid system. We cloned the full-length rat BAT3 cDNA from a fibroblast 3Y1 cDNA library. Our sequence analysis has demonstrated that rat BAT3 cDNA is 3617 nucleotides in length and encodes a full-length BAT3 (1098 amino acids) with an estimated molecular mass of 114,801 daltons, which displays an 87.4% identity with human BAT3. The deletion experiment revealed that the N-terminal region (amino acid residues 1-80) of DAN was required for the interaction with BAT3. Green fluorescent protein-tagged BAT3 was largely localized in the cytoplasm of COS cells. Northern hybridization showed that BAT3 mRNA was expressed in all the adult rat tissues examined but predominantly in testis. In addition, the level of BAT3 mRNA expression was more downregulated in some of the transformed cells, including v-mos- and v-Ha-ras-transformed 3Y1 cells, than in the parental cells.
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SUMMARY A hitherto unrecorded virus having flexible rod‐shaped particles about 740–760 × 13 nm was isolated from Anthoxanthum odoratwn L. It was transmitted by sap inoculation, but not by several species of insect, seed or soil to 18 species of Gramineae including wheat, oats and barley. In susceptible species the virus normally produced a mosaic mottling of the leaves which was sometimes followed by a necrotic streaking or striping.
Mosaic virus
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