Isolation of an elastase-like enzyme from skin lesions with Sweet's syndrome
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Isolation
Sweet's syndrome
The aim of this study was to investigate the presence of free elastase in GCF Samples were taken from inflamed sites in 12 subjects with gingivitis alone and from inflamed sites with and without tissue destruction in 19 patients having periodontitis. Elastase activity was measured with a low molecular weight substrate. To distinguish between free elastase and elastase bound to alpha-2-macroglobulin (A2MG), an excess of alpha-1-antitrypsin (A1AT) was added to the samples. The activity that could be inhibited by A1AT was considered as free elastase, and the uninhibited activity as derived from the elastase-A2MG complex. The elastase-A1AT complex was measured with an ELISA. Free elastase was found in almost all samples, but both the total amount and the proportion of free elastase were higher in samples from sites showing destruction. The elastase-A2MG complex was also increased in sites with tissue destruction, while there was no significant difference in the amount of A1AT complex between the 3 categories of sites. In conclusion, our study clearly reveals free elastase in GCF The elevated levels of free elastase in sites showing tissue destruction seem to be due to a combination of increased release of elastase and an inactivation of A1AT.
Pancreatic elastase
Neutrophil elastase
Alpha (finance)
SLPI
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Sweet's syndrome
Sweet Syndrome
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Concurrent Sweet's syndrome and acute sarcoidosis (Löfgren's syndrome) has been reported in 4 cases. We describe a 40-year-old woman with biopsy confirmed lesions of Sweet's syndrome and erythema nodosum together with arthritis and hilar and mediastinal adenopathy. We review the association of Sweet's syndrome and malignancy or hematologic disorders, and the need to exclude malignancy when hilar adenopathy is found. Aggressive diagnostic procedures can be avoided with prompt recognition of Löfgren's syndrome.
Sweet's syndrome
Erythema Nodosum
Sweet Syndrome
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ABSTRACT: Sweet's syndrome (acute neutrophilic dermatosis) is characterized by fever; polymorphonuclear neutrophilic teukocytosis; elevated ESR; and characteristic, raised, painful red plaques on the face and limbs. Since Sweet first described the syndrome in 1964; more than 150 cases have been reported, hut it is assumed that the disorder is far more common than this number would indicate.
Sweet's syndrome
Neutrophilic Dermatosis
Sweet Syndrome
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A 47-year-old man with a history of ulcerative colitis on prednisone and azathioprine was admitted to the hospital with a four-day history of fever, skin rash, arthralgias and leukocytosis. A skin biopsy demonstrated neutrophilic infiltration of the dermis that was consistent with Sweet's syndrome. He improved after several days with an increase in his prednisone and azathioprine. Sweet's syndrome is a rare cutaneous manifestation of inflammatory bowel disease, with approximately 40 cases reported in the literature. In a previously reported case of a patient with ulcerative colitis-associated Sweet's syndrome who was on azathioprine at the time of the skin eruption, the azathioprine was stopped, raising the possibility of drug-induced Sweet's syndrome. In the present case, the azathioprine was actually increased with complete resolution of the skin manifestations. This would support the theory that immunosuppressive therapy is the mainstay of therapy for this condition. In conclusion, Sweet's syndrome is a neutrophilic dermatosis that is rarely associated with ulcerative colitis. It may occur while on immunosuppressive therapy and responds to an intensification of immunosuppression.
Sweet's syndrome
Sweet Syndrome
Immunosuppression
Leukocytosis
Skin biopsy
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The effect of elastase on dermal elastic fibers from three age groups (children, young and old adults) has been investigated using ultrathin sections embedded in Epon. There were significant differences in the effect of elastase among the age groups; younger elastin was more rapidly digested by elastase than older elastin. In children and young adults, digested areas by elastase were first recognized as tiny round holes scattered in the amorphous material of the elastic fiber, extending to the large part of the fiber except for microfibrils. In old adults, digested areas were limited to the only small part of the elastic fiber.
Elastic fiber
Pancreatic elastase
Age groups
Digestion
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Sweet ì¦íêµ°ì ê¸ì± ì´ì± í¸ì¤êµ¬ì± í¼ë¶ì§íì¼ë¡ ë¤ìí ììììì ë³´ì¼ ì ìë¤. í¼í ëì¢ ì í¬í¨íë í¼ë¶ ë³ë³, ê´ì ì¼ ë° êµ¬ê°ë´ 궤ìì ëë°í Sweet ì¦íêµ°ì êµë´ë³´ê³ ê° ë물며 ì ìë¤ì ì´ìíì ì¡°ì§ ìê²ì íµí´ ì§ë¨ í ì¤í ë¡ì´ë ì ì í¬ì¬ë¡ í¸ì ë íì 1ì를 ê²½ííì기ì 문íê³ ì°°ê³¼ í¨ê» ë³´ê³ íë ë°ì´ë¤. ì¤ì¬ ë¨ì´: Sweet ì¦íêµ°; í¼í ëì¢ ; ê´ì ì¼
Sweet's syndrome
Sweet spot
Sweet Syndrome
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Plant protoplasts are isolated enzymically by the use of commercial cell wall degrading enzymes. These preparations are crude enzymes and, therefore, contain some toxic or undesirable substances. It is expected that highly purified enzymes may be useful for the isolation of intact protoplasts. However, it is not known what kinds of enzymes are actually required for the isolation of plant protoplasts. The answer to this question would be a prerequisite for the utilization of highly purified enzymes for the isolation.
Protoplast
Isolation
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Pancreatic elastase
Arterial wall
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Sweet's syndrome, originally described as an acute febrile neutrophilic dermatosis, belongs to a class of skin lesions that histologically have intense epidermal and/or dermal inflammatory infiltrate of neutrophils without evidence of infection or vasculitis. Skin lesions of Sweet's syndrome most commonly present on the face, trunk, upper extremities, and hands. The presenting lesions are often confused with infections because of their clinical appearance.A retrospective search of the electronic medical record was performed to identify patients with Sweet's syndrome from 1996 to the present. These records were then reviewed to identify those patients who had Sweet's syndrome that involved the hands.A total of 103 patients with Sweet's syndrome have been seen and treated at Scott and White Memorial Hospital since 1996. Of these, 49 patients had lesions on the hands. The presentation, treatment, and outcomes of several of these patients are presented.As physicians responsible for the treatment of hand lesions, it is important to consider the diagnosis of Sweet's syndrome because these wounds are unresponsive to antibiotics, do not benefit from débridement, and instead, require treatment with steroids.
Sweet's syndrome
Sweet Syndrome
Neutrophilic Dermatosis
Presentation (obstetrics)
Medical record
Skin lesion
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