The Heat-Shock Response and Expression of Heat-Shock Proteins in Wheat Under Diurnal Heat Stress and Field Conditions
33
Citation
0
Reference
10
Related Paper
Citation Trend
Abstract:
The occurrence of heat-shock proteins (HSPs) in response to high temperature stress is a universal phenomenon in higher plants and has been well documented. However, in agriculturally important species, less is known about the expression of HSPs under natural environments. A review of the heat-shock response in wheat (Triticum aestivum L.) is presented and recent results on the expression of wheat HSPs under diurnal stress and field conditions are reported. In the field experiment, flag leaf blade temperatures were obtained and leaf blades collected for northern blot analysis using HSP 16.9 cDNA as a probe. Temperatures of leaf blades ranged from 32 to 35�C under the tested field conditions at New Deal near Lubbock, Texas. Messenger RNAs encoding a major class of low molecular weight HSPs, HSP 16.9, were detected in all wheat genotypes examined. The results suggested that HSPs are synthesised in response to heat stress under agricultural production, and furthermore, that HSPs are produced in wheats differing in geographic background. In the controlled growth chamber experiment, HSP expression in two wheat cultivars, Mustang (heat tolerant) and Sturdy (heat susceptible) were analysed to determine if wheat genotypes differing in heat tolerance differ in in vitro HSP synthesis (translatable HSP mRNAs) under a chronic, diurnal heat-stress regime. Leaf tissues were collected from seedlings over a time-course and poly (A)+RNAs were isolated for in vitro translation and 2-D gel electrophoresis. The protein profiles shown in the 2-D gel analysis revealed that there were not only quantitative differences of individual HSPs between these two wheat lines, but also some unique HSPs which were only found in the heat tolerant line. This data provides evidence of a correlation between HSP synthesis and heat tolerance in wheat under a simulated field environment and suggests that further genetic analysis of HSPs in a segregating population is worthy of investigation. In conclusion, the results of this study provide an impetus for the investigation of the roles of HSP genes in heat tolerance in wheat.Keywords:
Thermal shock
Ecophysiology
HSP60
Northern blot
Ecophysiology
Plant Physiology
Cite
Citations (5)
The influence of CeO2 with different content on the thermal shock resistance of plasmasprayed Cr2O3 coating was investigated. The thermal shock failure mechanism of coating was also studied. It is found that the thermal shock failure mechanism of coating is thermal stress fatigue destruction, and the destruction takes place at interface of ceramic and bond coating. The experimental results show that the lifetime of coating fracture and failure increase considerably when 3% CeO2 is added into the plasma-sprayed Cr2O3 coating. The suitable content of CeO2 makes the microcracks exist in network form. The microcrack net can release internal stress in coating, delay the crack forming and expanding and decrease holes in coating. Thus the thermal shock resistance increase largely.
Thermal shock
Failure mechanism
Cite
Citations (0)
Steady state levels of the HSP70 transcript were followed by Northern hybridization in Moneuplotes crassus in order to investigate the mechanisms of the short term and long term response to heat shock in a spirotrichous ciliate. The influence of inhibitors of transcription or translation on the transcript levels was also studied. The heat shock response could be dissected into two phases. An initial protein-dependent stabilization of the mRNA was followed by an increase of the steady state transcript level that was dependent on continued transcription. As expected, the half-life of the RNA was short. Western blot analysis then showed that the HSP70 protein accumulated only upon permanent heat shock. It is concluded that the regulation of the heat shock response is a two-step process that occurs at the transcript level.
Northern blot
Transcription
Cite
Citations (4)
The influence of CeO 2 with different content on the thermal shock resistance of plasma sprayed Cr 2O 3 coating was investigated. The thermal shock failure mechanism of coating was also studied. It is found that the thermal shock failure mechanism of coating is thermal stress fatigue destruction, and the destruction takes place at interface of ceramic and bond coating. The experimental results show that the lifetime of coating fracture and failure increase considerably when 3%CeO 2 is added into the plasma sprayed Cr 2O 3 coating. The suitable content of CeO 2 makes the microcracks exist in network form. The microcrack net can release internal stress in coating, delay the crack forming and expanding and decrease holes in coating. Thus the thermal shock resistance increase largely.
Thermal shock
Failure mechanism
Cite
Citations (0)
Cells employ a well-preserved physiological stress response mechanism, termed the heat shock response, to activate a certain type of molecular chaperone called heat shock proteins (HSPs). HSPs are activated by transcriptional activators of heat shock genes known as heat shock factors (HSFs). These molecular chaperones are categorized as the HSP70 superfamily, which includes HSPA (HSP70) and HSPH (HSP110) families; the DNAJ (HSP40) family; the HSPB family (small heat shock proteins (sHSPs)); chaperonins and chaperonin-like proteins; and other heat-inducible protein families. HSPs play a critical role in sustaining proteostasis and protecting cells against stressful stimuli. HSPs participate in folding newly synthesized proteins, holding folded proteins in their native conformation, preventing protein misfolding and accumulation, and degrading denatured proteins. Ferroptosis is a recently identified type of oxidative iron-dependent cell demise. It was coined recently in 2012 by Stockwell Lab members, who described a special kind of cell death induced by erastin or RSL3. Ferroptosis is characterized by alterations in oxidative status resulting from iron accumulation, increased oxidative stress, and lipid peroxidation, which are mediated by enzymatic and non-enzymatic pathways. The process of ferroptotic cell death is regulated at multiple, and it is involved in several pathophysiological conditions. Much research has emerged in recent years demonstrating the involvement of HSPs and their regulator heat shock factor 1 (HSF1) in ferroptosis regulation. Understanding the machinery controlling HSF1 and HSPs in ferroptosis can be employed in developing therapeutic interventions for ferroptosis occurrence in a number of pathological conditions. Therefore, this review comprehensively summarized the basic characteristics of ferroptosis and the regulatory functions of HSF1 and HSPs in ferroptosis.
Proteostasis
HSF1
HSP60
Heat shock factor
Chaperone (clinical)
Proteotoxicity
HSPA4
Cite
Citations (17)
Plant ecophysiology is a branch of plant ecology,it combines the advantages of two disciplines of plant ecophysiology and plant ecology to analyze the ecological phenomena and solve the environmental problems.This paper reviews the research progress of plant ecophysiology from the studies of photosynthesis,adversity and laboratory instruments both at home and abroad,and forecasts its future research trends,which provides information for the related studies in China.
Ecophysiology
Plant science
Cite
Citations (1)
The thermal shock resistance of an Al\-2O\-3/(W,Ti)C functionally gradient ceramic material with symmetrical structure is investigated by means of water quench tests as well as the calculation of transient thermal stresses during thermal shock process.The results indicate that the thermal shock resistance of ceramic material can be improved by controlling the compositional distribution appropriately.
Thermal shock
Transient (computer programming)
Temperature Gradient
Cite
Citations (0)
An evaluation of thermal shock resistances for structural ceramics was conducted by employing various types of Vickers-indented specimens with different Biot moduli. Then, examination of the correlations between thermal shock resistances obtained by the author's method and usual thermal shock resistances, such as thermal shock fracture resistance and thermal shock damage resistance, was conducted. As a result, the correspondence between the minimum thermal shock condition required for the onset of propagation of generated microcracks and the thermal shock fracture resistance was recognized by conducting repeated thermal shock tests using thicker SiC specimens which have larger Biot moduli. The amount of crack growth per thermal shock cycle implies the same meanings as thermal shock damage resistance for various structural ceramics.
Thermal shock
Biot number
Temperature cycling
Cite
Citations (0)
The influence of additive CeO 2 with different content on the thermal shock resistance of plasma sprayed Cr 2O 3 coating was studied.It is found that the addition of 3.0%CeO 2 to Cr 2O 3 layer reduces the amount and the size of the pores in ceramic layer,and thus increases the resistance to thermal shock.The thermal shock failure of ceramic coating is essentially the fatigue failure induced by cyclic thermal stress during repeated heating and cooling.
Thermal shock
Cite
Citations (0)
Thermal shock
Residual strength
Alumina ceramic
Cite
Citations (21)