Serum ferritin concentration of dairy bulls: Determinations by radioimmunoassay and enzyme immunoassay.
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Serum ferritin concentration and other iron-related blood characteristics of Holstein dairy bulls were studied. Serum ferritin concentration was measured by a two-site immunoradiometric assay (2-site IRMA). The mean value ±standard error of this protein in blood serum of dairy bulls reared in breeding stations near to Sapporo was 36±7 ng/ml in adult (n=109) and 13±1 ng/ml in young bulls (n=19), respectively. Similar changes with age were observed in mean corpuscular volume, mean corpuscular hemoglobin, hemoglobin concentration, hematocrit, total serum protein and transferrin concentration. On the other hand, count of red blood cells in young bulls was higher than that in adult ones. But sexual difference in serum ferritin concentrations was not detected between bulls and cows previously reported. Furthermore, the ferritin levels in adult bulls were also measured by an enzyme immunoassay (EIA) used horseradish peroxidase as an enzyme and ABTS as a substrate. A correlation coefficient between ferritin concentrations determined by two methods was a significant high value (r=0.990). However, in the present EIA of serum ferritin, determination of low ferritin level below 5 ng/ml was likely difficult. But this assay system may be useful to determine high serum levels of ferritin in some metabolic disorders of animals.Keywords:
Immunoradiometric assay
Mean corpuscular hemoglobin
The present study was planned to investigate the effect of aluminium chloride and the protective effect of ginger extract on hematological profiles in male rats. One group of rats was given aluminium chloride orally (100mg/kg bw) for 60 days, another group with aluminium chloride and ginger extract (50mg/kg bw) simultaneously and the third group with ginger extract alone. All the three groups were compared with one control group. The hematological parameters were: total erythrocytes count (RBC), White blood cells (WBC), hemoglobin(HB), hematocrit (HCT), and red blood indices: mean corpuscular volume (MCV) mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHV) was investigated. The obtained results showed significant increase in WBC count and decrease in HCT, HB and RBC. No change was noted in (MCV) and there was reduction in mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHV) in AlCl3 administered group (E1). However, no appreciable changes were noted in AlCl3 and ginger extract treated (E2) and Ginger alone treated group (E3). The changes in the values of blood indices may be a defensive mechanism against aluminum toxicity through stimulation of erythropoisis. It may be concluded that Ginger rich in antioxidant and also spicy content which is safety food for human consumption. This study showed that hematologic effects of AlCl3 are reversible by aqueous ginger extract.
Mean corpuscular hemoglobin
Aluminium chloride
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Abstract Objective Recent studies suggest that chronic periodontitis can lead to a decrease in levels of red blood cell ( RBC ) and hemoglobin concentration. Smoking is an established risk factor for periodontitis. The present study was carried out to evaluate the effect of cigarette smoking on the various parameters of RBC in the subjects with chronic periodontitis. Methods A total of 77 males with chronic periodontitis were divided into 38 nonsmokers (group I) and 39 current smokers (group II ). Clinical parameters recorded were probing pocket depth and clinical attachment level. Laboratory blood investigations included hemoglobin concentration, RBC count, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration and erythrocyte sedimentation rate. Results In group II probing pocket depth and clinical attachment level and erythrocyte sedimentation rate were significantly higher than in group I . The RBC count, hemoglobin concentration, and packed cell volume were significantly lower in group II compared to group I . While the mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were comparable in both the groups. Conclusion Smoking may have an effect on levels of various RBC parameters in patients with chronic periodontitis.
Mean corpuscular hemoglobin
Erythrocyte sedimentation rate
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Season of the Year should be Considered in the Interpretation of Hematology in Carthusian Broodmares
This research was designed to provide data on the influence of the season on the year in the hematology of the Carthusian broodmares. Thirty-eight healthy Carthusian broodmares, aged 7-14 years were sampled during a year every two weeks. Therefore, a total of 24 blood samples were withdrawn for each animal. The following variables were measured: red blood cell count, hemoglobin concentration, packed cell volume, volumetric indexes (mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration), total white blood cell count and subtypes (neutrophils, lymphocytes, eosinophils, monocytes and basophils), platelet count and serum concentrations. Red blood cell count and packed cell volume were higher in spring. Mean corpuscular volume increased in autumn and mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration decreased in summer and autumn. Platelet count increased in summer. The highest numbers of neutrophils, lymphocytes and eosinophils were found in spring. Serum proteins and neutrophil/lymphocyte ratio remained statistically unchanged during the study. It is concluded that there is seasonality in the hematological parameters of the Carthusian broodmares.
Mean corpuscular hemoglobin
Hematology
White blood cell
Mean platelet volume
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Objectives: Hemoglobin & red blood cell indices (mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width) among adolescent. Study Design: Cross-Sectional study (Descriptive). Setting: Department of Physiology, Baqai Medical University (BMU) Karachi. Period: 6 months from February to August 2017. Material & Methods: A total of 500 students of MBBS, BDS, DPT with having age ranges from 18-25 years were enrolled in this study. The anthropometric measurement [height (m2) and weight (kg)] was recorded for calculation of the Body Mass Index and Complete blood count i-e Hemoglobin (Hb%), Mean Corpuscular Volume (MCV), Mean corpuscular hemoglobin (MCH), Mean corpuscular hemoglobin concentration (MCHC), Red cell distribution width (RDW) was done and calculated. Data analysis was done on Microsoft excel & SPSS version 22.0 was used. Results: It was seen that the comparison of Hb%, MCV & RDW had no significant (p>0.001) association of study participants to different categories of Body Mass Index. Mean corpuscular hemoglobin (MCH) (X²=28.278, p<0.001) and Mean corpuscular hemoglobin concentration (MCHC) (X²=15.659, p=0.016) were statistically significantly association with different categories of Body Mass Index. Conclusion: Mean corpuscular hemoglobin and Mean corpuscular hemoglobin concentration had statistically significant (p<0.001) association with body mass index (BMI).
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In this study, hematology was evaluated in jundiá fish males and females fed with three protein levels: 24, 27 and 30% CP. A total of 270 fishes (135 males and 135 females – initial weight of 216.38 ± 26.73 g) were fed during 100 days, in a water re-use system containing 18 tanks (six treatments and three replicates). After this period, six fishes by treatment were captured for blood collection, by which the following values were obtained: hematocrit, hemoglobin, red blood cells, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and platelets. The males showed higher values (P<0.01) of red blood cells, hematocit and hemoglobin comparing to the females. The females showed higher platelet values. Platelets and mean corpuscular hemoglobin concentration were affected by dietetic protein level. Corpuscular volume and mean corpuscular hemoglobin were not affected by the diet protein level nor the fish sex (P>0.05). It can be concluded that jundiá fish males showed higher hematological parameters compared to females. The dietary protein influences in platelets and mean corpuscular hemoglobin concentration of jundiá fish.
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Mean platelet volume
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Hematological parameters are repeatedly used as diagnostic and effective tools to assess the health status of fish and these parameters are not only varied with season but also varied between the sexes with in the species. Therefore, the purpose of the present study was to investigate the variation in hematological parameters of Schizothorax plagiostomus with respect to season and sex. This study was carried out in a stretch of time from 2014 to 2015 and the fish were collected from river Jhelum of Kashmir Himalayas. Blood was analyzed using different techniques and difference in hematological parameters including hemoglobin (Hb), total erythrocyte count (RBC), total leucocyte count (TLC), hematocrit (Hct), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) from 30 males (Weight, 311.62±25.76gm; length 33.5±3.26cm) and 31 females (Weight 335±32.55gm length 32.99±3.76cm) were reported during the present study. The results indicated that the highest hemoglobin concentration red blood cell count, hematocrit, mean corpuscular hemoglobin, mean corpuscular volume were found in males which were significant (p
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Mean corpuscular hemoglobin
Mean platelet volume
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Objective To survey and analyze the routine reference intervals of peripheral blood determination among healthy children.Methods A total of 2 408 healthy children from 2 to 6 years old were classified into several groups(2-,3-,4-and 5-),and the white blood cell,red blood cell,hemoglobin,hematocrit,mean corpuscular volume,mean corpuscular hemoglobin,mean corpuscular hemoglobin concentration of peripheral blood were determined.Reference intervals were obtained by statistical analysis of the parameters among the different age groups.Results Among the different age groups,the white blood cell was statistically significant(P0.05).However,separate reference intervals were not estimated,unless the difference was at least 25% between the subclass means and combination means.The other parameters had no statistical significance(P0.05).After the combination of the above parameters,the interval of white blood cell was 4.8-11.6(7.7) ×109/L,the interval of red blood cell was 3.75-5.18(4.47)×1012/L,the interval of hemoglobin was 110-145(126)g/L,the interval of hematocrit was 31.2-41.5(36.3)%,the interval of mean corpuscular volume was 76-88(81)fl,the interval of mean corpuscular hemoglobin was 25-31(28)pg,the interval of mean corpuscular hemoglobin concentration was 329-368(349)g/L,and the interval of platelet was 175-460(310)×109/L.The reference intervals of hemoglobin,red blood cell and hematocrit in Shanghai were similar to those in Changsha,and were lower than those in Kunming.The low and high limits of platelet in Shanghai were higher than those in Changsha and Kunming.Conclusions Different detection system should be established for the reference intervals in the different regions and different laboratories.
Mean corpuscular hemoglobin
White blood cell
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The purpose of this study is to establish hematological values in healthy workers. Seven thousand one hundred industrial workers, of which 4, 080 were male and 3, 020 were female, were investigated as to red blood cell count, hemoglobin content, hematocrit value and mean corpuscular constants (mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration). Frequency distributions of six sorts of hematological values in men were close to the normal. But different from those in men frequency distribution curves were skew and shifted to the left side in women. These tendencies of mean corpuscular constants were larger than those of red blood cell count, hemoglobin content and hematocrit value. The means and the standard deviations (and 5th-95th percentile) were 487.3±34.7 (430-544)×104/mm3 for red blood cell count in men, 421.6±31.1 (370-473)×104/mm3 for red blood cell count in women, 15.1±1.0 (13.3-16.8) g/dl for hemoglobin content in men, 12.4±1.1 (10.7-14.2) g/dl for hemoglobin content in women, 46.1±3.0 (41-51) % for hematocrit value in men, 38.5±3.0 (33-43) % for hematocrit value in women, 94.8±4.8 (87-103) μm3 for mean corpuscular volume in men, 91.1±6.0 (81-101) μm3 for mean corpuscular volume in women, 30.5±1.5 (28-33) μg for mean corpuscular hemoglobin in men, 29.2±2.1 (26-33) μg for mean corpuscular hemoglobin in women, 32.3±0.8 (31-34) % for mean corpuscular hemoglobin concentration in men and 32.1±0.9 (31-34) % for mean corpuscular hemoglobin concentration in women. Among the means of hematological values, red blood cell count and hemoglobin content in both men and women and hematocrit value in men tended to decline with age, but mean corpuscular volume and mean corpuscular hemoglobin in both men and women tended to increase with age. These differences by age were statistically significant. The sex differences of the means of all were statistically significant. The sex differences of the means of red blood cell count, hemoglobin content, and hematocrit value were larger than those of mean corpuscular constants.
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Venous blood samples were collected monthly from 60 male and 60 female Sinclair(S-1) miniature swine from 1 through 36 months of age. Hematologic parameters evaluated included: erythrocyte count, packed cell volume, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, leukocyte count and differential leukocyte counts. Mean erythrocyte count increased from 3 through 9 months of age and declined thereafter. Mean packed cell volume and hemoglobin concentration decreased from 1 to 3 months of age, increased from 3 through 10 months of age and remained stable thereafter. From 7 through 36 months of age, females had greater mean packed cell volume and hemoglobin concentration than did males. Mean corpuscular volume and mean corpuscular hemoglobin increased linearly from 3 through 24 months of age and remained stable thereafter with females having greater mean corpuscular volumes and mean corpuscular hemoglobins than did males. Mean corpuscular hemoglobin concentration increased linearly from 1 through 30 months of age. Mean leukocyte count decreased from 1 through 9 months of age and remained stable thereafter. From 10 through 36 months of age, mean leukocyte count was greater for males than for females. From 6 through 24 months of age, mean per cent lymphocytes was greater for females than for males; whereas, mean per cent neutrophils was greater for males than for females.
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