Simultaneous Determination of Saccharin and Aspartame in Drinks by UV-Spectrophotometry
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A simple and relatively less expensive UV spectrophotometric method was developed for simultaneous determination of saccharin and aspartame in soft drinks by multicomponent analysis method. Wavelengths of 235 nm and 257 nm were selected for determination of saccharin and aspartame respectively. The limit of detection for saccharin and aspartame was found to be 0.0015 g L−1 and 0.04 g L−1 respectively. The method obeys beers law within the concentration range of 0.002 g L−1 to 0.04 g L−1 and 0.06 g L−1 to 0.28 g L−1 for saccharin and aspartame respectively. This method was found to have the coefficient correlation (r2) value of 0.9997 and 0.999 for saccharin and aspartame respectively. The method was validated for accuracy, precision and repeatability. The proposed method was found to be simple, accurate, reproducible, sensitive, free form interferences, inexpensive and having good applicability to the routine analysis of samples of foods and beverages.Keywords:
Aspartame
Saccharin
Repeatability
Artificial Sweetener
Spectrophotometry
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A simple, cheap and sensitive spectrophotometric method for the determination of methyl paraben in pure and dosage form has been described. The Method is based on the diazotization of the drug by sodium nitrite in acidic medium at 5Co followed by coupling with ortho-aminobenzoic acid to form orange color the product was stabilized and measured at 442 nm Beer’s law is obeyed in the concentration range of 1-9 μg∙ml-1 with molar absorptivity of 1.6x106 L∙mole-1∙cm-1., Sandell’s sensitivity were 0.0095 μg∙cm-1, The detection limit were 0.0065 μg∙ml-1, and The limit of Quantitation were 0.02 μg∙ml-1. All variables including the reagent concentration, reaction time, color stability period, and mole ratio were studied in order to optimize the reaction conditions. No interferences were observed Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of methyl paraben in some oral solution. The developed method is easy to use and accurate for routine studies relative to HPLC and other techniques.
Paraben
Molar absorptivity
Phenolphthalein
Sodium nitrite
Spectrophotometry
Color reaction
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A simple spectrophotometric method has been developed for the determination of aspartame in table-top sweeteners and soft drinks based on the reaction of aspartame with ninhydrin. The selectivity of the method was improved through extraction with propylene carbonate. The optimum conditions for the extraction of aspartame and for the reaction of aspartame with ninhydrin in propylene carbonate were assessed. The absorbance measurements were made at 585 nm. The calibration graph was linear in the range 7.5–35.0 µg ml–1 of aspartame with a slope of 0.0358 (p.p.m.)–1. The precision of the procedure was checked by calculating the relative standard deviation of ten replicate determinations on a cola sample containing 680 µg ml–1 of aspartame, and was found to be 2.4%. Many common ingredients of soft drinks do not interfere, and the tolerable levels of several common free amino acids are increased through extraction with propylene carbonate. The method was applied to the determination of the aspartame content of various synthetic and real samples, and the results obtained for the real samples were compared with those obtained using high-performance liquid chromatography.
Aspartame
Ninhydrin
Absorbance
Standard curve
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Tartrazine
Spectrophotometry
Derivative (finance)
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Caffeine content was determined in cola, coffee and tea by second and third order derivative spectrophotometry without using any separation or background correction technique and reagent. The method is based on the measurement of the distances between two extremum values (peak to peak amplitudes) in the second order (cola) and third order (coffee and tea) derivative spectra of the sample solution. Calibration curves were constructed for the 2.0-10.0 m g ml-1concentration range. As a reference method, reversed phase high performance liquid chromatographic procedure was developed. Commercially available beverages were analyzed by the two methods and the results were statistically compared by using t- and F-tests.
Derivative (finance)
Spectrophotometry
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A simple, rapid, and sensitive spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners is proposed. This method is based on the reaction of saccharin with tetrachloro-p-benzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in an ethanol:acetone (4:1) medium, producing a violet-red compound (λmax = 550 nm). Beer's law is obeyed in a concentration range of 2.05 × 10-4 to 3.00 × 10-3 M with an excellent correlation coefficient (r = 0.9998). The detection limit was 1.55 × 10-5 M, and the effect of interferences on the spectrophotometric measurements was evaluated. The proposed procedure was applied successfully to the determination of saccharin in noncaloric sweeteners. Recoveries were within 99.2−104.3% with standard deviations ranging from to 0.5−1.6%. Results of the proposed method compare very favorably with those given by the high-performance liquid chromatography method recommended by the Food and Drug Administration. Keywords: Saccharin; noncaloric sweeteners; spectrophotometric determination
Saccharin
Sucralose
Benzocaine
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A simple, quick, and accurate new method for the determination of quinine (6′-methoxycinchonan-9-ol) in soft drinks is presented. The analysis is carried out using high performance liquid chromatography (HPLC), coupled laser induced fluorescence (LIF) that consisted of a 325 nm He-Cd laser and a ZETALIF detector. The chromatographic separation was performed on a Phenomenex Synergi Fusion-Reversed Phase (RP) column and allows good peak shape and symmetry in less than 1.5 min. A calibration curve ranging from 1 to 100 ng/mL was shown to be linear with a correlation coefficient (R) of 0.9999. The limit of detection of quinine was 3.2 pg on the column. The method was applied to the analysis of several beverages (n = 43) containing quinine, whose analysis required minimum pretreatment before direct injection, and can therefore be used for quality control in comparison to the classical methods. Data obtained from different commercial beverages containing quinine show no homogeneous concentration of this compound. This article describes, for the first time, the successful application of HPLC coupled LIF detection for quinine determination in common beverages.
Quinine
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Chemometrics
Principal component regression
Sodium bisulfite
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In this study, direct quantification of ethanol and methanol in distilled alcoholic beverages using Raman spectroscopy was performed. Raman spectra of varying ethanol–methanol mixtures were obtained, baseline corrections were made, and the data were normalized using Raman scattering intensity of an internal standard (acetonitrile, 921 cm –1 ). Then, calibration graphs were produced for ethanol and methanol concentrations in the ranges of 0–7 M and 0–10 M, respectively. Accurate R 2 values of the calibration graphs proved the notable linear correlations (0.998 for ethanol and 0.998 for methanol). The method was validated based on linearity, sensitivity, intraday and interday repeatability, and recovery tests. The limit of detection and limit of quantification values of the validated method were determined for ethanol concentration as 1.2 and 3.7 mM, and for methanol concentration as 3.4 and 10.3 mM, respectively. The ability of the developed method to detect ethanol and methanol concentrations in real samples was also investigated. The results of the developed method were compared with the experimental results from traditional method and high correlation value ( R 2 = 0.926) was obtained. Besides being sensitive and cheap, the developed method is rapid with the analysis time of less than 30 s. Furthermore, it eliminates labor‐consuming operations, chromatographic separation, and measurement error due to the high number of experiment steps in the standard method. Copyright © 2012 John Wiley & Sons, Ltd.
Repeatability
Distilled water
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Citations (118)
Mean centering and first derivative transforms of UV ratio spectra were developed to resolve strongly overlapping spectra of paracetamol and caffeine in binary mixtures. Paracetamol 25 mg/L and caffeine 3.25 mg/L were identified as suitable divisors to obtain highest spectral recovery. The calibration graphs were recorded in phosphate buffer pH 7.2 for paracetamol (10–35 mg/L) and caffeine (2.4–4.4 mg/L). These spectrophotometric methods were successfully applied to the simultaneous determination of paracetamol and caffeine in commercially available tablets. They were also more sensitive than previously reported spectrophotometric method. No significant difference in accuracy and precision was observed for the proposed methods and official USP-HPLC.
Phosphate buffered saline
Derivative (finance)
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A simple and fast approach is used for the first time to develop a time resolved lanthanide-sensitized luminescence method for the simultaneous determination of a preservative and a sweetener, namely benzoic acid (BZ) and saccharin (SC), respectively, in food samples. The method involves the formation of the corresponding ternary chelates with terbium(III) and trioctylphosphine oxide (TOPO) in the presence of Triton X-100, and the measurement of the initial rate and equilibrium signal of this system, which were obtained in 0.1 and 5 s, respectively. The dynamic ranges of the calibration graphs, obtained by using kinetic and equilibrium measurements, were 0.2–36 µg ml–1 and 0.15–30 µg ml–1, respectively, for BZ, and 3.3–24 µg ml–1 and 4–36 µg ml–1 for SC and the detection limits were 0.07 and 0.04 µg ml–1, respectively, for BZ, and 1.1 and 1.2 µg ml–1, respectively, for sodium SC. The relative standard deviation ranged between 2.3 and 3.0%. Both compounds were determined simultaneously by using a system of two equations which were resolved by using the calibration data obtained individually for each analyte and by multiple linear regression. Mixtures of BZ and SC in ratios between 3:1 and 1:9 were satisfactorily resolved by using both approaches. The method was applied to the direct analysis of several soft drinks. Analytical recoveries ranged between 89.3 and 108.5%.
Trioctylphosphine oxide
Benzoic acid
Sorbic acid
Saccharin
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Citations (35)