Quantitative Analysis of Poly-Phenol and Flavonoid in Several Edible Flowers
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Total flavonoid and phenolic content, antiradical properties and external flavonoid profiles on extracts of six samples of flower heads of cultivated Arnica montana was assessed. The studied samples were compared in relation to location of cultivation, origin of the seeds, time of harvesting, method of propagation: in vitro and in vivo. Four flavonoid aglycones were detected of examined exudates by co-chromatography with known compounds - scutellarein-6-methyl ether (hispidulin), scutellarein 6,4’-dimethyl ether (pectolinarigenin), 6-hydroxyluteolin 6-methyl ether and kaempferol 6-methyl ether. Quality differences in flavonoid composition were not observed between the studied samples while quantitative variations of total flavonoid and phenolic content were detected but in the most cases they a statistically not significant. The highest antioxidant properties displayed the samples from in vivo grown plants. As a result of the present preliminary study it was established that the best values of studied parameters showed the sample of in vivo grown plants, from seeds with Ukrainian origin, cultivated in Rhodope Mountain, collected at full flowering stage.
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Objective: Tagetes erecta, marigold, has various ethnomedicinal uses. It has a wide variety of bioactive components such as polyphenols and flavonoids which show different bioactivities. The aim of the present study is to quantitatively estimate total phenolic content (TPC) and total flavonoid content (TFC) of different extracts of T. erecta flowers.Methods: Extraction was done by maceration process, sequentially from non-polar to polar. Chloroform, ethyl acetate, and methanol extracts of T. erecta flowers were subjected to preliminary phytochemical screening. The extracts were analyzed for TPC and TFC using gallic acid and rutin as standard, respectively.Result: Phytochemical screening of different extracts showed the presence of carbohydrates, flavonoids, phenolics, fats, and oils. TPC and TFC in extracts of T. erecta varies with solvents. The study revealed that methanolic extract possesses the highest phenolic content, 49.76±0.29 mg gallic acid equivalents/g extract, and also maximum flavonoid content, 13.43±0.43 mg RE/g extract, among the three extracts.Conclusion: Higher value of phenolics and flavonoid indicates higher antioxidant activity. The present study revealed that methanolic extract has the highest phenolic and flavonoid content. This indicates that the flowers may possess a good antioxidant property and further research could be carried out.
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