2Ip03 Gene cloning and molecular analysis of salivacin K21, a bacteriocin produced by thermotolerant Lactobacillus salivarius AC21
Chie Koga古賀 千恵Sonomura Nana園村 奈々Saki Ito伊藤 紗希Sunee Nitisinprasert善藤 威史中山 二郎Zendo Takeshi園元 謙二Nakayama JirouKenji Sonomoto松崎 弘美Matusaki Hiromi
0
Citation
0
Reference
20
Related Paper
Keywords:
Lactobacillus salivarius
Cloning (programming)
Cite
Cite
Citations (8)
Isolation
Strain (injury)
Cite
Citations (10)
Strain (injury)
Cite
Citations (31)
Cite
Citations (0)
Strains of Lactobacillus plantarum and Leuconostoc mesenteroides were tested for bacteriocin production against each other and a range of closely related bacteria. L. plantarum 1193 was found to produce an inhibitory substance active against L. plantarum 340 and 1752, L. mesenteroides 8015 and Pediococcus damnosus 1832. This substance is a potential bacteriocin and has been named plantacin B.
Cite
Citations (155)
Lactobacillus paracasei
Yeast extract
Cite
Citations (105)
A bacteriocin-producing strain was isolated from infant feces.With the morphological observation,physiological and biochemical characteristics and sequencing of the 16S rDNA,this strain was identified to be a gram-positive Lactobacillus plantarum.Bacteriocin activity was degraded by proteolytic enzymes (trypsin,proteinase K etc.),and it was stable at high temperatures (100℃,30 min),in a range of pH 2-4.The bacteriocin showed antimicrobial activity against Staphylococcus aureus,Escherichia coli,Salmonella species.
Proteolytic enzymes
Strain (injury)
Cite
Citations (0)
Molecular mass
Cite
Citations (44)
Cite
Citations (0)
Bacteriocin-producing lactic acid bacteria were isolated from bekasam, an Indonesian traditional fermented meat. One isolate producing potential bacteriocin (S12) was identified and named Lactobacillus plantarum based on 16S rRNA sequencing. The 16S rRNA sequence of S12 had 98% homology with those of other Lactobacillus plantarum strains. Bacteriocin S12 exhibited inhibition ability against mainly pathogenic bacteria Listeria monocytogenes and Staphylococcus aureus. Production of the bacteriocin S12 was growth-associated manner, started at the early exponential and reached its maximum activity at the early stationary phase. Bacteriocin S12 tended stable at high temperature up to 121 °C for 15 minutes. The molecular weight of bacteriocin S12 was estimated 10.79 kDa by SDS-PAGE analysis. Moreover, the genes encoding bacteriocin S12 have been identified as plantaricin genes plnF and plnJ. Therefore, these results suggest that the bacteriocin (plantaricin) S12 might belong to Class II (b) bacteriocin that has potential in food biopreservative.
Biopreservation
Cite
Citations (1)