Cloning and Characterization of a Gene Cluster Involved in the Biosynthesis of Polyketide Antibiotic Dihydrochalcomycin in Streptomyces sp. KCTC 0041BP
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(1) Background: Streptomyces sp. TP-A0598 derived from seawater produces lydicamycin and its congeners. We aimed to investigate its taxonomic status; (2) Methods: A polyphasic approach and whole genome analysis are employed; (3) Results: Strain TP-A0598 contained ll-diaminopimelic acid, glutamic acid, glycine, and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H6) and MK-9(H8), and the major fatty acids were C16:0, iso-C15:0, iso-C16:0, and anteiso-C15:0. Streptomyces sp. TP-A0598 showed a 16S rDNA sequence similarity value of 99.93% (1 nucleottide difference) to Streptomyces angustmyceticus NRRL B-2347T. The digital DNA-DNA hybridisation value between Streptomyces sp. TP-A0598 and its closely related type strains was 25%-46%. Differences in phenotypic characteristics between Streptomyces sp. TP-A0598 and its phylogenetically closest relative, S. angustmyceticus NBRC 3934T, suggested strain TP-A0598 to be a novel species. Streptomyces sp. TP-A0598 and S. angustmyceticus NBRC 3934T harboured nine and 13 biosynthetic gene clusters for polyketides and nonribosomal peptides, respectively, among which only five clusters were shared between them, whereas the others are specific for each strain; and (4) Conclusions: For strain TP-A0598, the name Streptomyces lydicamycinicus sp. nov. is proposed; the type strain is TP-A0598T (=NBRC 110027T).
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Here we report the draft genome sequence of Nocardiopsis sp. strain TP-A0876, isolated from marine sediment, which produces polyketide-derived pyrones called nocapyrones. The genome contains three polyketide synthase (PKS) gene clusters, one of which was proposed to be responsible for nocapyrone biosynthesis. This genome sequence will facilitate the study of the potential for secondary metabolism in Nocardiopsis strains.
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Abstract A Gram‐positive, moderately halophilic Streptomyces strain, designated JAJ06, was isolated from saltpan soil collected at Tuticorin, India, and subjected to a polyphasic characterization with an insight into their biotechnological importance. Growth characteristics and antimicrobial com‐pound producing capabilities of Streptomyces sp. JAJ06 were observed on various International Streptomyces Project (ISP) media and production media. Optimum growth was observed on modified ISP 4 medium supplemented with 4% NaCl (w/v) at 29 °C incubated for 7 days. Maximum antibacterial compound production with good mycelial growth was observed on starch‐yeast extract‐peptone medium prepared with seawater (90%, v/v). The 16S rRNA gene based phylogenetic affiliation was determined by using various bioinformatics tools and the strain was identified as Streptomyces sp. JAJ06 with 99% sequence similarity to Streptomyces radiopugnans T . An antimicrobial assay of antimicrobial compound derived from Streptomyces sp. JAJ06 against a set of bacteria and a yeast strain revealed antimicrobial activity with significant minimal inhibitory concentrations. The potential antimicrobial compound produced by Streptomyces sp. JAJ06 was found to be polyketide in nature. Cloning and sequence analysis of 613‐bp fragment of ketosynthase gene from the type‐II polyketide operon revealed that Streptomyces sp. JAJ06 has the KSα gene with 91% sequence similarity to the type II polyketide synthase gene of Streptomyces peucetius . (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
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We report here the draft genome sequence of Streptomyces sp. SPMA113 isolated from soil in Thailand. This strain produces a new modified peptide, prajinamide, which has adipocyte differentiation activity. The genome harbors at least 30 gene clusters for synthases of polyketide and nonribosomal peptide, suggesting its potential to produce diverse secondary metabolites.
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Streptomyces albus is the type species of the genus Streptomyces. Here, we report the draft genome sequence of S. albus strain NBRC 13014(T). The genome contains at least seven orphan polyketide synthase and nonribosomal peptide synthetase gene clusters. The genome sequence will also serve as a valuable reference for Streptomyces taxonomy.
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In order to obtain the natural products synthesized by the three putative xiamycin biosynthesis gene clusters which were predicted via antiSMASH during the genome mining of marine Streptomyces sp. FXJ 7.388, Streptomyces sp. FXJ 8.012, and Streptomyces olivaceus FXJ 7.023. Sixteen genes involved in xiamycin assembly, modification, and regulation with higher identity than the newest reported xiamycin biosynthetic gene cluster from marine Streptomyces sp. SCSIO 02999, Streptomyces sp. HKI0576, and Streptomyces sp. FXJ 7.388 were discovered via gene cluster comparative analysis. A ribosome engineering strategy was adopted to activate such cryptic gene clusters with different final concentrations antibiotics that act on the ribosome, and two indolosesquiterpenes were isolated from idlethaldose streptomycin-resistant Streptomyces sp. FXJ 7.388 strains. However, no such product was detected in Streptomyces sp. FXJ 8.012 and Streptomyces olivaceus FXJ 7.023 under the same treatment. This result suggested that these genes might hold the least gene content for xiamycin biosynthesis.
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