Outbreak of equine influenza in polo horses in Ibadan, Nigeria: virus isolation, clinical manifestation and diagnosis
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Equine influenza
Isolation
Virus isolation
Ten strains of influenza B virus isolated in a local focus during an influenza outbreak were found to include 9 virus strain variants as demonstrated by different antigenicity of their haemagglutinin, ts-marker, sensitivity to heating at 56 degrees C/30 min, and to non-specific serum inhibitors. These strains induced antibodies in rats which interacted more actively with the virus isolated in earlier periods of this outbreak than with that isolated later. It might indicate that all strains originated from the same parent strain of virus, which induced the influenza outbreak in this area.
Antigenicity
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H5N1 genetic structure
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Isolation &identification of Newcastle disease virus from wild pigeonsinhabiting University of Baghdad (Al-Khwarizmi College of engineering)where velogenic and devastating infection of birds was encountered. Chickenembryos fibroblasts (CEF) were used for virus isolation (from infectedspleen and lungs) and propagation. Viral cytopathogenic effect was noticedon infected (CEF) cell culture within (24) hr post inoculation. The virus wasidentified by heamagglutination inhibition (HI) test using reference antiNDV serum.
Newcastle Disease
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1. Three strains of virus were isolated in suckling mice from the blood of three patients suffering from dengue during a local outbreak of the disease. 2. One strain was adapted to adult mice and identified as a dengue virus of type 1. The identification was confirmed by workers in America. 3. Evidence is presented that the other two strains of virus are similar to the one fully identified. 4. Attention is drawn to the need for detailed work to find a more sensitive method of isolation for dengue viruses.
Isolation
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Ten strains of influenza B virus isolated in a local focus during an influenza outbreak were found to include 9 virus strain variants as demonstrated by different antigenicity of their haemagglutinin, ts-marker, sensitivity to heating at 56 degrees C/30 min, and to non-specific serum inhibitors. These strains induced antibodies in rats which interacted more actively with the virus isolated in earlier periods of this outbreak than with that isolated later. It might indicate that all strains originated from the same parent strain of virus, which induced the influenza outbreak in this area.
Antigenicity
Strain (injury)
H5N1 genetic structure
Influenzavirus B
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Isolation
Virus isolation
H5N1 genetic structure
Equine influenza
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Embryonated
Equine influenza
Medical microbiology
H5N1 genetic structure
Human influenza
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Isolation &identification of Newcastle disease virus from wild pigeonsinhabiting University of Baghdad (Al-Khwarizmi College of engineering)where velogenic and devastating infection of birds was encountered. Chickenembryos fibroblasts (CEF) were used for virus isolation (from infectedspleen and lungs) and propagation. Viral cytopathogenic effect was noticedon infected (CEF) cell culture within (24) hr post inoculation. The virus wasidentified by heamagglutination inhibition (HI) test using reference antiNDV serum.
Newcastle Disease
Isolation
Virus isolation
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Virus isolation
Pestivirus
Isolation
Togaviridae
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Thirty-one ponies immunized with inactivated virus vaccine containing A/equine/Miami/63 (H3N8) virus and six seronegative ponies were experimentally challenged with the homologous virus strain. All 6 unvaccinated ponies and 11 out of 31 vaccinated ponies became infected. A clear relationship between pre-challenge antibody, measured by single radial haemolysis (SRH), and protection was demonstrated as judged by virus excretion, febrile responses and antibody responses. Those ponies with SRH antibody levels greater than 74 mm2 were completely protected against challenge infection by the intranasal route.
Equine influenza
Haemolysis
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Sindbis virus
Isolation
Virus isolation
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