Effect of Calcium Soaps of Fatty Acids and Administration of Somatotropin on Milk Production, Preovulatory Follicular Development, and Plasma and Follicular Fluid Lipid Composition in High Yielding Dairy Cows
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Follicular fluid
Bovine somatotropin
GH is secreted in a pulsatile manner, the pattern of which plays an important role in the regulation of growth and metabolism. Sex steroids are also known to participate in metabolic regulation. The present study was undertaken to elucidate the relationship between changes in GH pulsatility and metabolic transition during the estrous cycle in goats. From ovariectomized (OVX) and intact females in the early luteal, late luteal, and follicular phases, blood samples were taken every 15 min for 24 h, and plasma GH was measured by RIA. In the early luteal phase, GH was secreted in a distinct pulsatile manner, the pattern of which was similar to that in OVX goats, whereas the GH pulse frequency, amplitude, and area under the curve (AUC) were decreased in the late luteal phase. In the follicular phase, the GH pulse frequency, amplitude, and AUC were significantly larger than those in the late luteal phase. The regularity of GH pulsatility was highest and lowest in the early and late luteal phases, respectively. Both IGF-I and free fatty acid levels in the plasma were higher in the follicular than the luteal phase. Subcutaneous injection of estradiol to OVX goats increased the GH pulse amplitude and AUC, whereas the implantation of progesterone for 5 d decreased those parameters. These results suggest that the pulsatile pattern of GH secretion in goats varies with sex steroid levels and thereby affects IGF-I secretion and lipolysis during the estrous cycle.
Pulsatile flow
Sex steroid
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Norepinephrine (NE) concentrations in the hypothalamus of female Sprague-Dawley rats with normal estrous cycles averaged about 2.2 μg/g of wet tissue and these values were similar at the 3 phases of the estrous cycle. Comparison of NE levels in the hypothalamus of the rat at the 3 different time periods on proestrous day—preceding, following and coincidingcoinciding with the presumed time of the release of the ovulatory quota of gonadotropins—did not reveal measurable changes in hypothalamic concentrations of the neurohumor. Similarly, no measurable changes in NE levels were found as a result of castration. {Endocrinology83: 1383,
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The present study was undertaken to assess the ability of granulosa cells from subjects with normal and polycys-tic ovaries (PCO) to secrete progesterone throughout a 10-day culture period. LH levels in serum and fdllicular fluid from PCO patients were significantly (P <0.001) higher than those in normal subjects. In the absence of LH, progesterone secretion by granulosa cells cultured from PCO follicles did not differ significantly from that of cells from normal early and midfol-licular phase follicles. Granulosa cells cultured from follicles from normal subjects in the early and midfollicular phases responded to LH (100 ng/ml) with an – to 20-fold increase in progesterone production. In contrast, LH increased progesterone production to a much lesser extent (up to 4-fold) in cells from the ovaries of patients with PCO. Progesterone secretion by granulosa cells from normal ovaries in response to LH diminished as intrafollicular endogenous progesterone and LH levels increased. Cells from PCO follicles cultured with (Bu)2cAMP (100 μg/ml) secreted progesterone in quantities comparable to those secreted by (Bu)2cAMP-stimulated normal ovaries in the early and midfollicular phases. These data demonstrate the discrepancy between the ability of granulosa cells from PCO and normal follicles to secrete progesterone in response to stimulation by LH and (Bu)2cAMP. These results suggest that in women with PCO, the persistant elevation of follicular LH may lead to impaired progesterone production in response to exogenous LH. (J Clin Endocrinol Metab63: 1156, 1986)
Cyclic adenosine monophosphate
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The present study was conducted to deduce the alteration in follicular dynamics, hormonal and biochemical profile across seasons in buffaloes. In this study, 14 cyclic buffaloes were selected for assessing the follicular dynamics, hormonal and biochemical profile, 7 each during summer and winter. Higher ambient temperature was observed in summer as compared to winter. Nonsignificant difference across seasons with respect to growth rate of follicles and duration of follicular waves was observed. Serum progesterone (P4) showed no significant difference across seasons but differed significantly with respect to days of the estrous cycle in both seasons. Cortisol level diddiffer significantly from day 4 to day 16 of estrous cycle across seasons. Follicular hormones, viz. estradiol and P4 were significantly higher irrespective of seasons as compared to peripheral level. Follicular biochemical parameters, viz. cholesterol, total protein significantly differed between serum and follicular fluid during summer. Similarly during winter, follicular glucose was higher as compared to serum. In conclusion, season has a significant effect on peripheral cortisol with nonsignificant alterations in follicular dynamics, follicular hormones (E2 and P4) and biochemical milieu across seasons in cyclic buffaloes.
Follicular fluid
Follicular Cyst
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ABSTRACT The effects of feed restriction and refeeding on ovarian and hepatic insulin-like growth factor-I (IGF-I) gene expression, systemic and ovarian IGF-I concentrations and on associated metabolic changes were measured in prepubertal gilts. Eleven pairs of littermate gilts (70·7 ± 4·7 kg) were placed on a maintenance level of feeding for 7 days (days 1–7). On day 8, littermates were either fed at a maintenance level of energy or fed to appetite for a further 6 days. Blood samples were taken on day 13 (07.00–16.00 h) to determine plasma insulin and IGF-I, and on day 14 (02.00–06.00 h) to determine plasma GH levels. Following slaughter on day 14, one ovary from each animal was retained to measure follicular fluid IGF-I and oestradiol concentrations. The remaining ovary and a sample of liver were retained for IGF-I mRNA analysis using a ribonuclease protection assay. Six days of refeeding significantly increased plasma IGF-I ( P <0·005) and basal insulin ( P <0·05) but there was no effect on plasma GH. Ovarian follicular volume and diameter were significantly larger after refeeding ( P <0·05), with no effect on follicular fluid oestradiol concentrations. Mean follicular fluid IGF-I concentrations were unaffected by treatment. However, the relationships between individual follicular IGF-I concentrations, absolute follicular fluid IGF-I contents and follicle volume were affected by feeding level ( P <0·05). Regression analysis of the same data also revealed that at this stage of maturity, small follicles had greater follicular fluid concentrations of IGF-I than larger follicles. Refeeding increased the amount of IGF-I mRNA in hepatic but not ovarian tissue. We conclude that there is differential regulation of the IGF-I gene in porcine hepatic and ovarian tissues, and that ovarian factors other than, or as well as, IGF-I are involved in the regulation of ovarian responses to refeeding. Journal of Endocrinology (1993) 139, 143–152
Follicular fluid
Basal (medicine)
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Levels of thyrotropin-releasing hormone (TRH), TRH mRNA and pyroglutamyl peptidase II were analyzed in the hypothalamus-adenohypophyseal axis during lactation and estrous cycle. Mediobasal hypothalamic levels of TRH dropped 41% (p < 0.01) from pregnancy levels (taken as 100%) on the first day of lactation, recovering until day 15 to the values observed at pregnancy. A sharp decrease was also observed during weaning (36%, p < 0.01 compared to last day of lactation). TRH levels in the neurohypophysis increased during lactation and dropped at weaning. Highest TRH mRNA levels in the paraventricular nucleus were found at the end of pregnancy and beginning of lactation; they decreased 37% (p < 0.05) at day 5 of lactation and stayed constant thereafter. Pyroglutamyl peptidase II adenohypophyseal activity was not modified during lactation but changed during estrous cycle. Relative to estrous values, activity diminished 58% (p < 0.05) at 10.00 h (57% at 14.00 h) during diestrus 2 and 27% at 10.00 h (37% at 14.00 h) during proestrus. Hypothalamic TRH mRNA levels fluctuated in an opposite manner to adenohypophyseal pyroglutamyl peptidase II during the estrous cycle with a peak at diestrus 2: 183% of the estrous value (p < 0.05). These data point to a regulation of TRH metabolism in conditions where prolactin (PRL) secretion fluctuates. They also suggest a sharp release of TRH between the end of pregnancy and the first day of lactation and that translational efficiency or post-translational processing of TRH precursor in the paraventricular neurons (projecting to the median eminence) increases during lactation and drops at weaning, concomitantly with PRL secretion.
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Changes in plasma concentrations of ACTH, beta-endorphin (beta-EP) and cortisol have been found to be associated during the human menstrual cycle. Changes in hypothalamic levels of gonadotrophin releasing hormone (GnRH), beta-EP and substance P (SP) have also been associated with the oestrous cycle in the rat. Therefore, an attempt was made to measure the activity of the corticotrophic axis and SP by measuring blood and follicular fluid concentrations of ACTH, beta-EP, SP and corticotrophin releasing hormone (CRH) during the hormonal ovarian stimulation phase for in-vitro fertilization (IVF), in a series of 19 patients. At the plasma level, there was no significant change over treatment days in ACTH (P = 0.1550), beta-EP (P = 0.1137), or SP concentrations (P = 0.5625). CRH was not detectable over treatment days. In addition, there was no significant change in neuropeptide over treatment days between those women who became pregnant and those who did not (P = 0.17 for all). In the follicular fluid, ACTH was not detectable, beta-EP concentration was three times higher than in the plasma, CRH was detectable, and SP concentration was similar to that of plasma. There was no apparent correlation, however, between beta-EP or SP concentrations in the plasma and follicular fluid from a given patient. In conclusion, the absence of changes in the activity of the corticotrophic axis during the hormonal ovarian stimulation suggests that there was no major stress component associated with the stimulation phase of IVF or the occurrence of a pregnancy.
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beta-Endorphin
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The time-related changes in gonadotrophin concentrations after a single injection of steroid-free bovine follicular fluid (bFF), which contains material with inhibin-like activity, were studied in 25-day-old and adult female rats which either were intact or had been ovariectomized 2 days before. In ovariectomized and intact rats administration of bFF caused a selective suppression of FSH after 4–8 h in 25-day-old rats and after 3–4 h in adult rats. No systematic changes in concentrations of LH after bFF injection were observed. Relative suppression of FSH levels in adult rats was more pronounced and of longer duration than in 25-day-old rats. Moreover, the total period of suppression lasted longer in ovariectomized than in intact rats (12 and 8 h for 25-day-old and 24 and 15 h for adult rats respectively). Hypersecretion of FSH was found in intact rats after the initial suppression; this phenomenon was more pronounced and of longer duration in adult than in 25-day-old rats. No clear change in the numbers of healthy growing follicles was observed after injection of bFF into intact rats. These results indicate that the pituitary secretion of FSH responds quickly and selectively after administration of bFF to intact and ovariectomized, 25-day-old and adult female rats. The hypersecretion of FSH in intact rats might compensate for the initial suppression of this gonadotrophin, and may thus ensure the maturation of a normal number of follicles.
Follicular fluid
Adult male
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Melanocyte-stimulating hormone
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Conceptus
Bovine somatotropin
Follicular fluid
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